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. 1994 May 15;300(Pt 1):81–84. doi: 10.1042/bj3000081

Inhibition of inositol trisphosphate-induced calcium release by caffeine is prevented by ATP.

L Missiaen 1, J B Parys 1, H De Smedt 1, B Himpens 1, R Casteels 1
PMCID: PMC1138126  PMID: 8198556

Abstract

We have investigated the effect of various methylxanthines on the basal and InsP3-stimulated unidirectional 45Ca2+ efflux from permeabilized A7r5 cells under different experimental conditions. We report that caffeine and theophylline inhibit the InsP3-induced Ca2+ release, whereas the basal Ca2+ leak remained largely unaffected. The effect on InsP3-induced Ca2+ release was specific, since isocaffeine was not effective. The inhibition occurred similarly in the absence or presence of extravesicular Ca2+ and was not associated with a decrease in the [3H]InsP3 binding to the receptor. ATP and MgATP (5 mM) prevented the inhibition, suggesting that caffeine may interact with an ATP-binding site on the InsP3 receptor or some associated protein.

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Selected References

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