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. 1994 May 15;300(Pt 1):141–145. doi: 10.1042/bj3000141

Synthesis, purification and kinetic properties of fluorescein-labelled penicillins.

B Lakaye 1, C Damblon 1, M Jamin 1, M Galleni 1, S Lepage 1, B Joris 1, J Marchand-Brynaert 1, C Frydrych 1, J M Frere 1
PMCID: PMC1138135  PMID: 8198525

Abstract

The synthesis and properties of six fluorescein-labelled penicillins are reported. The two isomers of fluoresceyl-glycyl-6-amino-penicillanic acid are probably the best compounds to use for detection of all the penicillin-binding proteins (PBPs) present in a bacterial membrane preparation. However, the derivatives of ampicillin were much more efficient against Enterobacter aerogenes PBP3. The two isomers obtained when a commercial mixture of the two isomers of carboxyfluorescein was used most often exhibited similar properties, but the Streptomyces R61 extracellular DD-peptidase was only efficiently acylated by the 5'-carboxyfluorescein derivative of glycyl-6-aminopenicillanic acid.

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Selected References

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