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. 1989 Mar 1;258(2):595–598. doi: 10.1042/bj2580595

A denaturation-induced proton-uptake study of horse ferricytochrome c.

R T Hartshorn 1, G R Moore 1
PMCID: PMC1138402  PMID: 2539812

Abstract

The observation that 6 M-urea denatures horse ferricytochrome c in the pH range 4-6, but not horse ferrocytochrome c, has been exploited to determine the denaturation-induced proton uptake of ferricytochrome c. This is related to the pKa values of ionizable groups buried within the native protein. The data indicate that one of the haem propionic acid substituents of ferricytochrome c has a pKa of less than 4.5, whereas the other has a pKa of greater than 9.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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