Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1989 Mar 1;258(2):607–610. doi: 10.1042/bj2580607

Evidence for altered expression of the GTP-dependent regulatory proteins, Gs and Gi, in adipocytes from aged rats.

A Green 1, J L Johnson 1
PMCID: PMC1138404  PMID: 2495794

Abstract

The alpha-subunits of Gi and Gs were quantified in adipocyte membranes from young (2-month) and older (18-month) rats by pertussis-toxin and cholera-toxin labelling respectively. Aging was associated with a 3-fold increase in Gi alpha-subunit, but only a 2-fold increase in one of the two Gs alpha-subunit species labelled. The findings may explain the altered sensitivity of adipocytes from aged rats to lipolytic and anti-lipolytic stimuli.

Full text

PDF
607

Images in this article

608Fig. 1.
on p.608
608Fig. 2.
on p.608

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Daly J. W. Forskolin, adenylate cyclase, and cell physiology: an overview. Adv Cyclic Nucleotide Protein Phosphorylation Res. 1984;17:81–89. [PubMed] [Google Scholar]
  3. Dax E. M., Partilla J. S., Gregerman R. I. Mechanism of the age-related decrease of epinephrine-stimulated lipolysis in isolated rat adipocytes: beta-adrenergic receptor binding, adenylate cyclase activity, and cyclic AMP accumulation. J Lipid Res. 1981 Aug;22(6):934–943. [PubMed] [Google Scholar]
  4. Ebert R., Schwabe U. Studies on the antilipolytic effect of adenosine and related compounds in isolated fat cells. Naunyn Schmiedebergs Arch Pharmacol. 1973;278(3):247–259. doi: 10.1007/BF00500286. [DOI] [PubMed] [Google Scholar]
  5. Gilman A. G. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615–649. doi: 10.1146/annurev.bi.56.070187.003151. [DOI] [PubMed] [Google Scholar]
  6. Giudicelli Y., Pecquery R. beta-Adrenergic receptors and catecholamine-sensitive adenylate cyclase in rat fat-cell membranes: influence of growth, cell size and aging. Eur J Biochem. 1978 Oct;90(2):413–419. doi: 10.1111/j.1432-1033.1978.tb12619.x. [DOI] [PubMed] [Google Scholar]
  7. Green A. Adenosine receptor down-regulation and insulin resistance following prolonged incubation of adipocytes with an A1 adenosine receptor agonist. J Biol Chem. 1987 Nov 15;262(32):15702–15707. [PubMed] [Google Scholar]
  8. Ho R. J., Shi Q. H. Evidence for a single forskolin-binding site in rat adipocyte membrane. Studies of [14,15-3H]dihydroforskolin binding and adenylate cyclase activation. J Biol Chem. 1984 Jun 25;259(12):7630–7636. [PubMed] [Google Scholar]
  9. Hoffman B. B., Chang H., Farahbakhsh Z. T., Reaven G. M. Age-related decrement in hormone-stimulated lipolysis. Am J Physiol. 1984 Dec;247(6 Pt 1):E772–E777. doi: 10.1152/ajpendo.1984.247.6.E772. [DOI] [PubMed] [Google Scholar]
  10. Hoffman B. B., Chang H., Farahbakhsh Z., Reaven G. Inhibition of lipolysis by adenosine is potentiated with age. J Clin Invest. 1984 Nov;74(5):1750–1755. doi: 10.1172/JCI111593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Houslay M. Dual control of adenylate cyclase. Nature. 1983 May 12;303(5913):133–133. doi: 10.1038/303133a0. [DOI] [PubMed] [Google Scholar]
  12. Iyengar R., Rich K. A., Herberg J. T., Grenet D., Mumby S., Codina J. Identification of a new GTP-binding protein. A Mr = 43,000 substrate for pertussis toxin. J Biol Chem. 1987 Jul 5;262(19):9239–9245. [PubMed] [Google Scholar]
  13. Katada T., Oinuma M., Ui M. Mechanisms for inhibition of the catalytic activity of adenylate cyclase by the guanine nucleotide-binding proteins serving as the substrate of islet-activating protein, pertussis toxin. J Biol Chem. 1986 Apr 15;261(11):5215–5221. [PubMed] [Google Scholar]
  14. Katada T., Oinuma M., Ui M. Two guanine nucleotide-binding proteins in rat brain serving as the specific substrate of islet-activating protein, pertussis toxin. Interaction of the alpha-subunits with beta gamma-subunits in development of their biological activities. J Biol Chem. 1986 Jun 25;261(18):8182–8191. [PubMed] [Google Scholar]
  15. Kather H., Bieger W., Michel G., Aktories K., Jakobs K. H. Human fat cell lipolysis is primarily regulated by inhibitory modulators acting through distinct mechanisms. J Clin Invest. 1985 Oct;76(4):1559–1565. doi: 10.1172/JCI112137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Lakatta E. G. Catecholamines and cardiovascular function in aging. Endocrinol Metab Clin North Am. 1987 Dec;16(4):877–891. [PubMed] [Google Scholar]
  18. Linden J. Purification and characterization of (-)[125I]hydroxyphenylisopropyladenosine, an adenosine R-site agonist radioligand and theoretical analysis of mixed stereoisomer radioligand binding. Mol Pharmacol. 1984 Nov;26(3):414–423. [PubMed] [Google Scholar]
  19. Munshi R., Baer H. P. Radioiodination of p-hydroxyphenylisopropyladenosine: development of a new ligand for adenosine receptors. Can J Physiol Pharmacol. 1982 Oct;60(10):1320–1322. doi: 10.1139/y82-196. [DOI] [PubMed] [Google Scholar]
  20. Newby A. C., Luzio J. P., Hales C. N. The properties and extracellular location of 5'-nucleotidase of the rat fat-cell plasma membrane. Biochem J. 1975 Mar;146(3):625–633. doi: 10.1042/bj1460625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Okajima F., Ui M. ADP-ribosylation of the specific membrane protein by islet-activating protein, pertussis toxin, associated with inhibition of a chemotactic peptide-induced arachidonate release in neutrophils. A possible role of the toxin substrate in Ca2+-mobilizing biosignaling. J Biol Chem. 1984 Nov 25;259(22):13863–13871. [PubMed] [Google Scholar]
  22. Parsons W. J., Stiles G. L. Heterologous desensitization of the inhibitory A1 adenosine receptor-adenylate cyclase system in rat adipocytes. Regulation of both Ns and Ni. J Biol Chem. 1987 Jan 15;262(2):841–847. [PubMed] [Google Scholar]
  23. RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
  24. Schwabe U., Ebert R., Erbler H. C. Adenosine release from fat cells: effect on cyclic AMP levels and hormone actions. Adv Cyclic Nucleotide Res. 1975;5:569–584. [PubMed] [Google Scholar]
  25. Schwabe U., Lenschow V., Ukena D., Ferry D. R., Glossmann H. [125I] N6-p-Hydroxyphenylisopropyladenosine, a new ligand for Ri adenosine receptors. Naunyn Schmiedebergs Arch Pharmacol. 1982 Oct;321(1):84–87. doi: 10.1007/BF00586356. [DOI] [PubMed] [Google Scholar]
  26. Stanley K. K., Edwards M. R., Luzio J. P. Subcellular distribution and movement of 5'-nucleotidase in rat cells. Biochem J. 1980 Jan 15;186(1):59–69. doi: 10.1042/bj1860059. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES