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. 1989 Mar 15;258(3):837–841. doi: 10.1042/bj2580837

Cell fractionation studies indicate that dystrophin is a protein of surface membranes of skeletal muscle.

G Salviati 1, R Betto 1, S Ceoldo 1, E Biasia 1, E Bonilla 1, A F Miranda 1, S Dimauro 1
PMCID: PMC1138440  PMID: 2658974

Abstract

We studied the subcellular localization of dystrophin in rabbit skeletal muscle. In Western-blot analysis of membrane preparations, dystrophin was associated with the sarcolemmal fraction, as indicated by cholesterol content and co-purification with ouabain-binding activity and beta-adrenergic receptor. Dystrophin was also found with junctional T-tubules, but not with 'free' T-tubules, longitudinal portions or terminal cisternae of the sarcoplasmic reticulum. Dystrophin was not solubilized by high salt solutions, but it was solubilized by low concentrations of detergents (Triton X-100 and deoxycholate), suggesting that it is a peripheral membrane protein.

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