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. 1989 Apr 1;259(1):277–281. doi: 10.1042/bj2590277

Effects of antibody to 5 S-RNA-binding protein on protein synthesis in Artemia salina ribosomes.

N Kenmochi 1, Y Takahashi 1, N L Sato 1
PMCID: PMC1138501  PMID: 2719645

Abstract

The effects of an affinity-purified polyclonal antibody to Artemia salina ribosomal protein L5 on protein synthesis in vitro were examined. The antibody interacted with 60 S subunits more strongly than with 80 S ribosomes, and inhibited reassociation of ribosomal subunits to some extent at 5 mM-Mg2+ but not at 10 mM. Polyphenylalanine synthesis in vitro at 10 mM-Mg2+ was significantly inhibited, especially when the antibody was first preincubated with 60 S subunits prior to the assay. The incorporation of amino acid directed by globin mRNA was inhibited only when the preincubation with 60 S subunits was carried out. On the other hand, no effect was detected on elongation factor 2- and 60 S subunit-dependent uncoupled GTPase activity. These results suggest that L5 is probably located at or near the subunit interface and may play an important role in protein synthesis.

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Selected References

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