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. 1989 Apr 15;259(2):605–607. doi: 10.1042/bj2590605

The effects of acute-phase inducers and dimethyl sulphoxide on delta-aminolaevulinate synthase activity in human HepG2 hepatoma cells.

F Iwasa 1, S Sassa 1, A Kappas 1
PMCID: PMC1138553  PMID: 2541694

Abstract

The effects of acute-phase inducers and dimethyl sulphoxide (Me2SO) on delta-aminolaevulinate (ALA) synthase in HepG2 cells were examined. Treatment of cells with Me2SO resulted in a significant increase in ALA synthase activity. Interleukin-6 increased ALA synthase activity only slightly, but it substantially potentiated the induction of ALA synthase by Me2SO. These data suggest that ALA synthase activity in liver is altered during acute-phase reactions.

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Selected References

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  1. Baumann H., Richards C., Gauldie J. Interaction among hepatocyte-stimulating factors, interleukin 1, and glucocorticoids for regulation of acute phase plasma proteins in human hepatoma (HepG2) cells. J Immunol. 1987 Dec 15;139(12):4122–4128. [PubMed] [Google Scholar]
  2. Bissell D. M., Hammaker L. E. Cytochrome p-450 heme and the regulation of delta-aminolevulinic acid synthetase in the liver. Arch Biochem Biophys. 1976 Sep;176(1):103–112. doi: 10.1016/0003-9861(76)90145-4. [DOI] [PubMed] [Google Scholar]
  3. Brooker J. D., Srivastava G., May B. K., Elliott W. H. Radiochemical assay for gamma-aminolevulinate synthase. Enzyme. 1982;28(2-3):109–119. doi: 10.1159/000459095. [DOI] [PubMed] [Google Scholar]
  4. Falzon M., Milton A. S., Burke M. D. Are the decreases in hepatic cytochrome P-450 and other drug-metabolising enzymes caused by indomethacin in vivo mediated by intestinal bacterial endotoxins? 16,16-Dimethylprostaglandin F2 alpha prevents decreases in hepatic drug-metabolising enzymes due to exogenous endotoxin. Biochem Pharmacol. 1984 Apr 15;33(8):1285–1292. doi: 10.1016/0006-2952(84)90182-5. [DOI] [PubMed] [Google Scholar]
  5. Galbraith R. A., Sassa S., Fujita H. Regulation of heme synthesis in HepG2 human hepatoma cells by dimethyl sulfoxide. Biochem Biophys Res Commun. 1988 Jun 16;153(2):869–874. doi: 10.1016/s0006-291x(88)81176-8. [DOI] [PubMed] [Google Scholar]
  6. Galbraith R. A., Sassa S., Kappas A. Induction of haem synthesis in Hep G2 human hepatoma cells by dimethyl sulphoxide. A transcriptionally activated event. Biochem J. 1986 Jul 15;237(2):597–600. doi: 10.1042/bj2370597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grieninger G., Pindyck J., Hertzberg K. M., Mosesson M. W. Application of electroimmunoassay to the study of plasma protein synthesis in cultured hepatocytes. Ann Clin Lab Sci. 1979 Nov-Dec;9(6):511–517. [PubMed] [Google Scholar]
  8. Griffeth L. K., Rosen G. M., Tschanz C., Rauckman E. J. Effects of model traumatic injury on hepatic drug metabolism in the rat. I. In vivo antipyrine metabolism. Drug Metab Dispos. 1983 Nov-Dec;11(6):517–525. [PubMed] [Google Scholar]
  9. Iwasa F., Galbraith R. A., Sassa S. Effects of dimethyl sulphoxide on the synthesis of plasma proteins in the human hepatoma HepG2. Induction of an acute-phase-like reaction. Biochem J. 1988 Aug 1;253(3):927–930. doi: 10.1042/bj2530927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Nebert D. W., Gonzalez F. J. P450 genes: structure, evolution, and regulation. Annu Rev Biochem. 1987;56:945–993. doi: 10.1146/annurev.bi.56.070187.004501. [DOI] [PubMed] [Google Scholar]
  12. Renton K. W., Mannering G. J. Depression of hepatic cytochrome P-450-dependent monooxygenase systems with administered interferon inducing agents. Biochem Biophys Res Commun. 1976 Nov 22;73(2):343–348. doi: 10.1016/0006-291x(76)90713-0. [DOI] [PubMed] [Google Scholar]
  13. Sassa S. Delta-aminolevulinic acid dehydratase assay. Enzyme. 1982;28(2-3):133–145. doi: 10.1159/000459097. [DOI] [PubMed] [Google Scholar]
  14. Sassa S., Kappas A. Induction of aminolevulinate synthase and porphyrins in cultured liver cells maintained in chemically defined medium. Permissive effects of hormones on induction process. J Biol Chem. 1977 Apr 10;252(7):2428–2436. [PubMed] [Google Scholar]
  15. Stanley L. A., Adams D. J., Lindsay R., Meehan R. R., Liao W., Wolf C. R. Potentiation and suppression of mouse liver cytochrome P-450 isozymes during the acute-phase response induced by bacterial endotoxin. Eur J Biochem. 1988 May 16;174(1):31–36. doi: 10.1111/j.1432-1033.1988.tb14058.x. [DOI] [PubMed] [Google Scholar]
  16. Tanaka E., Kurata N., Kohno M., Yoshida T., Kuroiwa Y. Induction of cytochrome P-450 and related drug-oxidizing activities in muscone (3-methylcyclopentadecanone)-treated rats. Biochem Pharmacol. 1987 Dec 15;36(24):4263–4267. doi: 10.1016/0006-2952(87)90668-x. [DOI] [PubMed] [Google Scholar]

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