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. 1989 May 1;259(3):737–741. doi: 10.1042/bj2590737

Amplification of fluoroaluminate-stimulated inositol phosphate generation in a cell line overexpressing the p21N-ras gene.

M J Wakelam 1
PMCID: PMC1138580  PMID: 2658978

Abstract

The stimulation of inositol phosphate generation in NIH-3T3 cells and a derived transformant overexpressing the p21N-ras gene (T15+ cells) was examined. Incubation with NaF in the presence of Al3+ leads to the generation of inositol phosphates in each cell type, though the response in the T15+ cells is significantly amplified. The effect of fluoroaluminate is dose- and time-dependent. No differences were observed in fluoroaluminate-stimulated cyclic AMP accumulation among the cell types. In another NIH-3T3-derived cell line that expresses the transforming lys61 mutant of N-ras, no amplification of fluoroaluminate-stimulated inositol phosphate generation is observed. These results provide support for the proposal that, in the T15 cell line, p21N-ras can act in a guanine nucleotide-binding regulatory protein (G-protein)-like manner.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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