Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1989 May 15;260(1):271–275. doi: 10.1042/bj2600271

Glucocorticoids modulate mRNA levels for G-protein beta-subunits.

M Ros 1, D C Watkins 1, P J Rapiejko 1, C C Malbon 1
PMCID: PMC1138656  PMID: 2505749

Abstract

Adrenalectomy decreases, whereas glucocorticoid treatment increases, the steady-state levels of G-protein beta-subunits (G beta) in rat fat-cells. A DNA-excess solution-hybridization assay was established to define the steady-state mRNA levels for G beta [5.8 +/- 0.4 amol/micrograms of RNA (n = 5) in control fat-cells]. G beta mRNA levels decrease by 20% after adrenalectomy; dexamethasone treatment reverses the decline. Dexamethasone treatment itself increases G beta mRNA levels by 50%.

Full text

PDF
271

Images in this article

272Fig. 1.
on p.272

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  2. Codina J., Stengel D., Woo S. L., Birnbaumer L. Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical. FEBS Lett. 1986 Oct 27;207(2):187–192. doi: 10.1016/0014-5793(86)81486-7. [DOI] [PubMed] [Google Scholar]
  3. Evans T., Brown M. L., Fraser E. D., Northup J. K. Purification of the major GTP-binding proteins from human placental membranes. J Biol Chem. 1986 May 25;261(15):7052–7059. [PubMed] [Google Scholar]
  4. Fong H. K., Amatruda T. T., 3rd, Birren B. W., Simon M. I. Distinct forms of the beta subunit of GTP-binding regulatory proteins identified by molecular cloning. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3792–3796. doi: 10.1073/pnas.84.11.3792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. García-Sáinz J. A., Huerta-Bahena M. E., Malbon C. C. Hepatocyte beta-adrenergic responsiveness and guanine nucleotide-binding regulatory proteins. Am J Physiol. 1989 Feb;256(2 Pt 1):C384–C389. doi: 10.1152/ajpcell.1989.256.2.C384. [DOI] [PubMed] [Google Scholar]
  6. Gilman A. G. G proteins and dual control of adenylate cyclase. Cell. 1984 Mar;36(3):577–579. doi: 10.1016/0092-8674(84)90336-2. [DOI] [PubMed] [Google Scholar]
  7. Gilman A. G. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615–649. doi: 10.1146/annurev.bi.56.070187.003151. [DOI] [PubMed] [Google Scholar]
  8. Gilman A. G. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615–649. doi: 10.1146/annurev.bi.56.070187.003151. [DOI] [PubMed] [Google Scholar]
  9. Hadcock J. R., Malbon C. C. Down-regulation of beta-adrenergic receptors: agonist-induced reduction in receptor mRNA levels. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5021–5025. doi: 10.1073/pnas.85.14.5021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hadcock J. R., Malbon C. C. Regulation of beta-adrenergic receptors by "permissive" hormones: glucocorticoids increase steady-state levels of receptor mRNA. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8415–8419. doi: 10.1073/pnas.85.22.8415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jones D. T., Reed R. R. Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium. J Biol Chem. 1987 Oct 15;262(29):14241–14249. [PubMed] [Google Scholar]
  12. Katada T., Oinuma M., Ui M. Mechanisms for inhibition of the catalytic activity of adenylate cyclase by the guanine nucleotide-binding proteins serving as the substrate of islet-activating protein, pertussis toxin. J Biol Chem. 1986 Apr 15;261(11):5215–5221. [PubMed] [Google Scholar]
  13. Linnenbach A. J., Speicher D. W., Marchesi V. T., Forget B. G. Cloning of a portion of the chromosomal gene for human erythrocyte alpha-spectrin by using a synthetic gene fragment. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2397–2401. doi: 10.1073/pnas.83.8.2397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Logothetis D. E., Kurachi Y., Galper J., Neer E. J., Clapham D. E. The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart. Nature. 1987 Jan 22;325(6102):321–326. doi: 10.1038/325321a0. [DOI] [PubMed] [Google Scholar]
  15. Malbon C. C., Rapiejko P. J., Mangano T. J. Fat cell adenylate cyclase system. Enhanced inhibition by adenosine and GTP in the hypothyroid rat. J Biol Chem. 1985 Feb 25;260(4):2558–2564. [PubMed] [Google Scholar]
  16. Malbon C. C., Rapiejko P. J., Watkins D. C. Permissive hormone regulation of hormone-sensitive effector systems. Trends Pharmacol Sci. 1988 Jan;9(1):33–36. doi: 10.1016/0165-6147(88)90240-4. [DOI] [PubMed] [Google Scholar]
  17. Milligan G., Spiegel A. M., Unson C. G., Saggerson E. D. Chemically induced hypothyroidism produces elevated amounts of the alpha subunit of the inhibitory guanine nucleotide binding protein (Gi) and the beta subunit common to all G-proteins. Biochem J. 1987 Oct 1;247(1):223–227. doi: 10.1042/bj2470223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mochly-Rosen D., Chang F. H., Cheever L., Kim M., Diamond I., Gordon A. S. Chronic ethanol causes heterologous desensitization of receptors by reducing alpha s messenger RNA. Nature. 1988 Jun 30;333(6176):848–850. doi: 10.1038/333848a0. [DOI] [PubMed] [Google Scholar]
  19. Neer E. J., Clapham D. E. Roles of G protein subunits in transmembrane signalling. Nature. 1988 May 12;333(6169):129–134. doi: 10.1038/333129a0. [DOI] [PubMed] [Google Scholar]
  20. Ros M., Northup J. K., Malbon C. C. Adipocyte G-proteins and adenylate cyclase. Effects of adrenalectomy. Biochem J. 1989 Feb 1;257(3):737–744. doi: 10.1042/bj2570737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ros M., Northup J. K., Malbon C. C. Steady-state levels of G-proteins and beta-adrenergic receptors in rat fat cells. Permissive effects of thyroid hormones. J Biol Chem. 1988 Mar 25;263(9):4362–4368. [PubMed] [Google Scholar]
  22. Saggerson E. D. Sensitivity of adipocyte lipolysis to stimulatory and inhibitory agonists in hypothyroidism and starvation. Biochem J. 1986 Sep 1;238(2):387–394. doi: 10.1042/bj2380387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shackleford G. M., Varmus H. E. Expression of the proto-oncogene int-1 is restricted to postmeiotic male germ cells and the neural tube of mid-gestational embryos. Cell. 1987 Jul 3;50(1):89–95. doi: 10.1016/0092-8674(87)90665-9. [DOI] [PubMed] [Google Scholar]
  25. Sternweis P. C., Robishaw J. D. Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain. J Biol Chem. 1984 Nov 25;259(22):13806–13813. [PubMed] [Google Scholar]
  26. Stiles G. L., Stadel J. M., De Lean A., Lefkowitz R. J. Hypothyroidism modulates beta adrenergic receptor adenylate cyclase interactions in rat reticulocytes. J Clin Invest. 1981 Dec;68(6):1450–1455. doi: 10.1172/JCI110397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Williams D. L., Newman T. C., Shelness G. S., Gordon D. A. Measurement of apolipoprotein mRNA by DNA-excess solution hybridization with single-stranded probes. Methods Enzymol. 1986;128:671–689. doi: 10.1016/0076-6879(86)28099-4. [DOI] [PubMed] [Google Scholar]
  28. Yamamoto K. R. Steroid receptor regulated transcription of specific genes and gene networks. Annu Rev Genet. 1985;19:209–252. doi: 10.1146/annurev.ge.19.120185.001233. [DOI] [PubMed] [Google Scholar]
  29. Yatani A., Imoto Y., Codina J., Hamilton S. L., Brown A. M., Birnbaumer L. The stimulatory G protein of adenylyl cyclase, Gs, also stimulates dihydropyridine-sensitive Ca2+ channels. Evidence for direct regulation independent of phosphorylation by cAMP-dependent protein kinase or stimulation by a dihydropyridine agonist. J Biol Chem. 1988 Jul 15;263(20):9887–9895. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES