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. 1989 May 15;260(1):291–294. doi: 10.1042/bj2600291

Prostaglandin E2 inhibits phosphoinositide metabolism in isolated pancreatic islets.

S G Laychock 1
PMCID: PMC1138660  PMID: 2549955

Abstract

Isolated islets of the rat labelled with myo-[3H]inositol showed decreased accumulation of total inositol phosphates (InsPs) and [3H]polyphosphoinositide hydrolysis in response to glucose after preincubation with prostaglandin E2 (PGE2). The response was concentration-dependent and specific for PGE2. PGE2 did not affect basal [3H]phosphoinositide hydrolysis or InsPs accumulation. Pertussis-toxin pretreatment antagonized the response to PGE2, whereas 8-bromo cyclic AMP was without effect. The PGE2-induced decrease in InsPs may contribute to the suppression of insulin secretion.

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Selected References

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  1. Berridge M. J., Dawson R. M., Downes C. P., Heslop J. P., Irvine R. F. Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochem J. 1983 May 15;212(2):473–482. doi: 10.1042/bj2120473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Best L., Malaisse W. J. Nutrient and hormone-neurotransmitter stimuli induce hydrolysis of polyphosphoinositides in rat pancreatic islets. Endocrinology. 1984 Nov;115(5):1814–1820. doi: 10.1210/endo-115-5-1814. [DOI] [PubMed] [Google Scholar]
  3. Blachier F., Malaisse W. J. Phospholipase C activation via a GTP-binding protein in tumoral islet cells stimulated by carbamylcholine. Experientia. 1987 Jun 15;43(6):601–602. doi: 10.1007/BF02126344. [DOI] [PubMed] [Google Scholar]
  4. Chaudhry A., Thompson R. H., Rubin R. P., Laychock S. G. Relationship between delta-9-tetrahydrocannabinol-induced arachidonic acid release and secretagogue-evoked phosphoinositide breakdown and Ca2+ mobilization of exocrine pancreas. Mol Pharmacol. 1988 Oct;34(4):543–548. [PubMed] [Google Scholar]
  5. Cockcroft S., Stutchfield J. G-proteins, the inositol lipid signalling pathway, and secretion. Philos Trans R Soc Lond B Biol Sci. 1988 Jul 26;320(1199):247–265. doi: 10.1098/rstb.1988.0075. [DOI] [PubMed] [Google Scholar]
  6. Dunlop M. E., Larkins R. G. Muscarinic-agonist and guanine nucleotide activation of polyphosphoinositide phosphodiesterase in isolated islet-cell membranes. Biochem J. 1986 Dec 15;240(3):731–737. doi: 10.1042/bj2400731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans M. H., Pace C. S., Clements R. S., Jr Endogenous prostaglandin synthesis and glucose-induced insulin secretion from the adult rat pancreatic islet. Diabetes. 1983 Jun;32(6):509–515. doi: 10.2337/diab.32.6.509. [DOI] [PubMed] [Google Scholar]
  8. Hoffman J. M., Laychock S. G. Choline turnover in phosphatidylcholine of pancreatic islets. Implications for CDP-choline pathway. Diabetes. 1988 Nov;37(11):1489–1498. doi: 10.2337/diab.37.11.1489. [DOI] [PubMed] [Google Scholar]
  9. Laychock S. G. Effects of guanosine 3',5'-monophosphate on glucose utilization in isolated islets of Langerhans. Endocrinology. 1987 Feb;120(2):517–524. doi: 10.1210/endo-120-2-517. [DOI] [PubMed] [Google Scholar]
  10. Laychock S. G. Identification and metabolism of polyphosphoinositides in isolated islets of Langerhans. Biochem J. 1983 Oct 15;216(1):101–106. doi: 10.1042/bj2160101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laychock S. G. Prostaglandin E2 and alpha 2 adrenoceptor agonists inhibit the pentose phosphate shunt in pancreatic islets. Arch Biochem Biophys. 1989 Feb 15;269(1):354–358. doi: 10.1016/0003-9861(89)90117-3. [DOI] [PubMed] [Google Scholar]
  12. Matsuyama T., Horie H., Namba M., Nonaka K., Tarui S. Glucose dependent stimulation by prostaglandin D2 of glucagon and insulin in perfused rat pancreas. Life Sci. 1983 Feb 28;32(9):979–982. doi: 10.1016/0024-3205(83)90928-1. [DOI] [PubMed] [Google Scholar]
  13. Partke H. J., Wasner H. K. Cyclic AMP-antagonist, a second messenger for insulin action, inhibits glucose-stimulated insulin secretion in isolated islets of Chinese hamsters. Adv Exp Med Biol. 1986;211:409–412. doi: 10.1007/978-1-4684-5314-0_39. [DOI] [PubMed] [Google Scholar]
  14. Pek S., Lands W. E., Akpan J., Hurley M. Effects of prostaglandins H2, D2, I2, and thromboxane on in vitro secretion of glucagon and insulin. Adv Prostaglandin Thromboxane Res. 1980;8:1295–1298. [PubMed] [Google Scholar]
  15. Pek S., Tai T. Y., Elster A. Stimulatory effects of prostaglandins E-1, E-2, and F-2-alpha on glucagon and insulin release in vitro. Diabetes. 1978 Aug;27(8):801–809. doi: 10.2337/diab.27.8.801. [DOI] [PubMed] [Google Scholar]
  16. Robertson R. P., Chen M. A role for prostaglandin E in defective insulin secretion and carbohydrate intolerance in diabetes mellitus. J Clin Invest. 1977 Sep;60(3):747–753. doi: 10.1172/JCI108827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robertson R. P. Hypothesis. PGE, carbohydrate homeostasis, and insulin secretion. A suggested resolution of the controversy. Diabetes. 1983 Mar;32(3):231–234. doi: 10.2337/diab.32.3.231. [DOI] [PubMed] [Google Scholar]
  18. Robertson R. P., Tsai P., Little S. A., Zhang H. J., Walseth T. F. Receptor-mediated adenylate cyclase-coupled mechanism for PGE2 inhibition of insulin secretion in HIT cells. Diabetes. 1987 Sep;36(9):1047–1053. doi: 10.2337/diab.36.9.1047. [DOI] [PubMed] [Google Scholar]
  19. Robertson R. In vivo insulin secretion: prostaglandin and adrenergic interrelationships. Prostaglandins. 1974 Jun 25;6(6):501–508. doi: 10.1016/s0090-6980(74)80060-2. [DOI] [PubMed] [Google Scholar]
  20. Turk J., Hughes J. H., Easom R. A., Wolf B. A., Scharp D. W., Lacy P. E., McDaniel M. L. Arachidonic acid metabolism and insulin secretion by isolated human pancreatic islets. Diabetes. 1988 Jul;37(7):992–996. doi: 10.2337/diab.37.7.992. [DOI] [PubMed] [Google Scholar]
  21. Turk J., Wolf B. A., McDaniel M. L. Glucose-induced accumulation of inositol trisphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer. Biochem J. 1986 Jul 1;237(1):259–263. doi: 10.1042/bj2370259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wolf B. A., Comens P. G., Ackermann K. E., Sherman W. R., McDaniel M. L. The digitonin-permeabilized pancreatic islet model. Effect of myo-inositol 1,4,5-trisphosphate on Ca2+ mobilization. Biochem J. 1985 May 1;227(3):965–969. doi: 10.1042/bj2270965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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