Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1989 Jun 1;260(2):531–534. doi: 10.1042/bj2600531

Characterization of isoelectric subspecies of asialo-beta 2-glycoprotein I.

A Gries 1, J Nimpf 1, H Wurm 1, G M Kostner 1, T Kenner 1
PMCID: PMC1138700  PMID: 2764887

Abstract

Isoelectric focusing of purified beta 2-glycoprotein I (beta 2-G-I) revealed five major bands with isoelectric points (pI) between 5.1 and 6.1. Neuraminidase treatment decreased the number of bands to two (pI 8.0 and 8.2). The two asialo subfractions of beta 2-G-I were purified by cation-exchange column chromatography. The more basic isoform II was found to have a higher content of lysine. Western-blot analysis of different plasma samples confirmed the heterogeneity of beta 2-G-I in plasma. Plasma treated with neuraminidase showed two bands irrespective of the number of isoforms as well as of the concentration in native plasma. This led us to the conclusion that human plasma beta 2-G-I consists of two isoproteins that are sialylated to different extents.

Full text

PDF
531

Images in this article

532Fig. 1.
on p.532
532Fig. 2.
on p.532
533Fig. 3.
on p.533
534Fig. 5.
on p.534

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkin J., Rundle A. T. Serum beta 2-glycoprotein 1 phenotype frequencies in an English population. Humangenetik. 1974 Jan 22;21(1):81–84. doi: 10.1007/BF00278570. [DOI] [PubMed] [Google Scholar]
  2. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  3. Finlayson J. S., Mushinski J. F. Separation of subfractions of human beta-2-glycoprotein I. Biochim Biophys Acta. 1967 Dec 12;147(3):413–420. doi: 10.1016/0005-2795(67)90001-3. [DOI] [PubMed] [Google Scholar]
  4. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982 Jan 1;119(1):142–147. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
  5. Kamboh M. I., Ferrell R. E., Sepehrnia B. Genetic studies of human apolipoproteins. IV. Structural heterogeneity of apolipoprotein H (beta 2-glycoprotein I). Am J Hum Genet. 1988 Mar;42(3):452–457. [PMC free article] [PubMed] [Google Scholar]
  6. Koppe A. L., Walter H., Chopra V. P., Bajatzadeh M. Investigations on the genetics and population genetics of the beta 2-glycoprotein I polymorphism. Humangenetik. 1970;9(2):164–171. doi: 10.1007/BF00278932. [DOI] [PubMed] [Google Scholar]
  7. Laurell C. B. Electroimmuno assay. Scand J Clin Lab Invest Suppl. 1972;124:21–37. doi: 10.3109/00365517209102748. [DOI] [PubMed] [Google Scholar]
  8. Nimpf J., Bevers E. M., Bomans P. H., Till U., Wurm H., Kostner G. M., Zwaal R. F. Prothrombinase activity of human platelets is inhibited by beta 2-glycoprotein-I. Biochim Biophys Acta. 1986 Oct 29;884(1):142–149. doi: 10.1016/0304-4165(86)90237-0. [DOI] [PubMed] [Google Scholar]
  9. Richter A., Cleve H. Genetic variations of human serum beta 2-glycoprotein I demonstrated by isoelectric focusing. Electrophoresis. 1988 Jul;9(7):317–322. doi: 10.1002/elps.1150090706. [DOI] [PubMed] [Google Scholar]
  10. Schousboe I. Binding of beta 2-glycoprotein I to platelets: effect of adenylate cyclase activity. Thromb Res. 1980 Jul 1;19(1-2):225–237. doi: 10.1016/0049-3848(80)90421-1. [DOI] [PubMed] [Google Scholar]
  11. Schousboe I. Characterization of subfractions of beta 2-glycoprotein I: evidence for sialic acid microheterogeneity. Int J Biochem. 1983;15(1):35–44. doi: 10.1016/0020-711x(83)90008-3. [DOI] [PubMed] [Google Scholar]
  12. Schousboe I. Characterization of the interaction between beta 2-glycoprotein I and mitochondria, platelets, liposomes and bile acids. Int J Biochem. 1983;15(12):1393–1401. doi: 10.1016/0020-711x(83)90070-8. [DOI] [PubMed] [Google Scholar]
  13. Schousboe I. Effect of beta 2-glycoprotein I on the activity of adenylate cyclase in platelet membranes. Thromb Res. 1983 Nov 1;32(3):291–299. doi: 10.1016/0049-3848(83)90164-0. [DOI] [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  16. Warnick G. R., Mayfield C., Albers J. J., Hazzard W. R. Gel isoelectric focusing method for specific diagnosis of familial hyperlipoproteinemia type 3. Clin Chem. 1979 Feb;25(2):279–284. [PubMed] [Google Scholar]
  17. Wurm H. beta 2-Glycoprotein-I (apolipoprotein H) interactions with phospholipid vesicles. Int J Biochem. 1984;16(5):511–515. doi: 10.1016/0020-711x(84)90168-x. [DOI] [PubMed] [Google Scholar]
  18. Zechner R., Moser R., Kostner G. M. Isolation of pure LpB from human serum. J Lipid Res. 1986 Jun;27(6):681–686. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES