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. 1989 Jun 1;260(2):613–616. doi: 10.1042/bj2600613

The magnetic properties of the nickel cofactor F430 in the enzyme methyl-coenzyme M reductase of Methanobacterium thermoautotrophicum.

M R Cheesman 1, D Ankel-Fuchs 1, R K Thauer 1, A J Thompson 1
PMCID: PMC1138715  PMID: 2504147

Abstract

Cofactor 430 of methyl-coenzyme M reductase from Methanobacterium thermoautotrophicum was studied in both the extracted form in aqueous solution and protein-bound by using low-temperature magnetic-circular-dichroism spectroscopy. In both forms the nickel was present as high-spin paramagnetic nickel(II), spin S = 1, subject to almost equal zero-field splitting (cofactor F430, D = +9.0 cm-1, E/D = 0; methyl-coenzyme M reductase, D = +8.5 cm-1, [E/D[ = 0.2). This suggests identical axial co-ordination by oxygen ligand(s) both in aqueous cofactor F430 and in the investigated state of the protein.

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Selected References

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  1. Ankel-Fuchs D., Thauer R. K. Methane formation from methyl-coenzyme M in a system containing methyl-coenzyme M reductase, component B and reduced cobalamin. Eur J Biochem. 1986 Apr 1;156(1):171–177. doi: 10.1111/j.1432-1033.1986.tb09563.x. [DOI] [PubMed] [Google Scholar]
  2. Bobik T. A., Olson K. D., Noll K. M., Wolfe R. S. Evidence that the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate is a product of the methylreductase reaction in Methanobacterium. Biochem Biophys Res Commun. 1987 Dec 16;149(2):455–460. doi: 10.1016/0006-291x(87)90389-5. [DOI] [PubMed] [Google Scholar]
  3. Ellefson W. L., Whitman W. B., Wolfe R. S. Nickel-containing factor F430: chromophore of the methylreductase of Methanobacterium. Proc Natl Acad Sci U S A. 1982 Jun;79(12):3707–3710. doi: 10.1073/pnas.79.12.3707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ellermann J., Hedderich R., Böcher R., Thauer R. K. The final step in methane formation. Investigations with highly purified methyl-CoM reductase (component C) from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1988 Mar 15;172(3):669–677. doi: 10.1111/j.1432-1033.1988.tb13941.x. [DOI] [PubMed] [Google Scholar]
  5. Jones W. J., Nagle D. P., Jr, Whitman W. B. Methanogens and the diversity of archaebacteria. Microbiol Rev. 1987 Mar;51(1):135–177. doi: 10.1128/mr.51.1.135-177.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rouvière P. E., Wolfe R. S. Novel biochemistry of methanogenesis. J Biol Chem. 1988 Jun 15;263(17):7913–7916. [PubMed] [Google Scholar]
  7. Schönheit P., Moll J., Thauer R. K. Nickel, cobalt, and molybdenum requirement for growth of Methanobacterium thermoautotrophicum. Arch Microbiol. 1979 Oct;123(1):105–107. doi: 10.1007/BF00403508. [DOI] [PubMed] [Google Scholar]
  8. Shiemke A. K., Hamilton C. L., Scott R. A. Structural heterogeneity and purification of protein-free F430 from the cytoplasm of Methanobacterium thermoautotrophicum. J Biol Chem. 1988 Apr 25;263(12):5611–5616. [PubMed] [Google Scholar]
  9. Thomson A. J., Johnson M. K. Magnetization curves of haemoproteins measured by low-temperature magnetic-circular-dichroism spectroscopy. Biochem J. 1980 Nov 1;191(2):411–420. doi: 10.1042/bj1910411. [DOI] [PMC free article] [PubMed] [Google Scholar]

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