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. 1989 Jul 1;261(1):89–92. doi: 10.1042/bj2610089

Metabolism of inositol phosphates in alpha 1-adrenoceptor-stimulated and homogenized cardiac myocytes of adult rats.

A H Guse 1, I Berg 1, G Gercken 1
PMCID: PMC1138785  PMID: 2549967

Abstract

Previous studies demonstrated the accumulation of inositol mono- and poly-phosphates in carbamoylcholine-stimulated cultured cardiac ventricular myocytes of adult rats [Berg, Guse & Gercken (1989) Biochim. Biophys. Acta 1010, 100-107]. Stimulation with noradrenaline (50 microM) in the presence of propranolol (10 microM) led to a time-dependent pattern of inositol mono- and poly-phosphates in cultured cardiac-ventricular myocytes. Ins(1,4,5)P3 and Ins(1,3,4,5)P4 increased in a rapid initial phase. The degradation products of Ins(1,4,5)P3, namely Ins(1,4)P2 and Ins(4)P, accumulated between 1 and 15 min. Ins(1,3,4,5)P4 was rapidly dephosphorylated to Ins(1,3,4)P3, which was then metabolized to Ins(1,3)P2 and Ins(3,4)P2. The last two InsP2 isomers and their degradation products, Ins(1)P and Ins(3)P (determined as an enantiomeric mixture), increased at extended stimulation periods. To confirm the pathway of Ins(1,3,4,5)P4 degradation, homogenates of isolated ventricular myocytes were incubated with [3H]INs(1,3,4,5)P4. The subsequent products were Ins(1,3,4)P3, Ins(3,4)P2, Ins(1,3)P2 and InsP. The effect of noradrenaline was antagonized by prazosin (0.1 microM), but not by yohimbine (0.1 microM), indicating phosphoinositidase activation via the alpha 1-adrenoceptor.

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Selected References

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  1. Berg I., Guse A. H., Gercken G. Carbamoylcholine-induced accumulation of inositol mono-, bis-, tris- and tetrakisphosphates in isolated cardiac myocytes from adult rats. Biochim Biophys Acta. 1989 Jan 17;1010(1):100–107. doi: 10.1016/0167-4889(89)90189-4. [DOI] [PubMed] [Google Scholar]
  2. Dean N. M., Moyer J. D. Metabolism of inositol bis-, tris-, tetrakis- and pentakis-phosphates in GH3 cells. Biochem J. 1988 Mar 1;250(2):493–500. doi: 10.1042/bj2500493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dean N. M., Moyer J. D. Separation of multiple isomers of inositol phosphates formed in GH3 cells. Biochem J. 1987 Mar 1;242(2):361–366. doi: 10.1042/bj2420361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jones L. G., Goldstein D., Brown J. H. Guanine nucleotide-dependent inositol trisphosphate formation in chick heart cells. Circ Res. 1988 Feb;62(2):299–305. doi: 10.1161/01.res.62.2.299. [DOI] [PubMed] [Google Scholar]
  5. Joseph S. K., Hansen C. A., Williamson J. R. Inositol 1,3,4,5-tetrakisphosphate increases the duration of the inositol 1,4,5-trisphosphate-mediated Ca2+ transient. FEBS Lett. 1987 Jul 13;219(1):125–129. doi: 10.1016/0014-5793(87)81203-6. [DOI] [PubMed] [Google Scholar]
  6. Koppitz B., Vogel F., Mayr G. W. Mammalian aldolases are isomer-selective high-affinity inositol polyphosphate binders. Eur J Biochem. 1986 Dec 1;161(2):421–433. doi: 10.1111/j.1432-1033.1986.tb10462.x. [DOI] [PubMed] [Google Scholar]
  7. Legssyer A., Poggioli J., Renard D., Vassort G. ATP and other adenine compounds increase mechanical activity and inositol trisphosphate production in rat heart. J Physiol. 1988 Jul;401:185–199. doi: 10.1113/jphysiol.1988.sp017157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morgan R. O., Chang J. P., Catt K. J. Novel aspects of gonadotropin-releasing hormone action on inositol polyphosphate metabolism in cultured pituitary gonadotrophs. J Biol Chem. 1987 Jan 25;262(3):1166–1171. [PubMed] [Google Scholar]
  9. Nosek T. M., Williams M. F., Zeigler S. T., Godt R. E. Inositol trisphosphate enhances calcium release in skinned cardiac and skeletal muscle. Am J Physiol. 1986 May;250(5 Pt 1):C807–C811. doi: 10.1152/ajpcell.1986.250.5.C807. [DOI] [PubMed] [Google Scholar]
  10. Poggioli J., Sulpice J. C., Vassort G. Inositol phosphate production following alpha 1-adrenergic, muscarinic or electrical stimulation in isolated rat heart. FEBS Lett. 1986 Oct 6;206(2):292–298. doi: 10.1016/0014-5793(86)80999-1. [DOI] [PubMed] [Google Scholar]
  11. Renard D., Poggioli J. Does the inositol tris/tetrakisphosphate pathway exist in rat heart? FEBS Lett. 1987 Jun 8;217(1):117–123. doi: 10.1016/0014-5793(87)81254-1. [DOI] [PubMed] [Google Scholar]
  12. Scholz J., Schaefer B., Schmitz W., Scholz H., Steinfath M., Lohse M., Schwabe U., Puurunen J. Alpha-1 adrenoceptor-mediated positive inotropic effect and inositol trisphosphate increase in mammalian heart. J Pharmacol Exp Ther. 1988 Apr;245(1):327–335. [PubMed] [Google Scholar]
  13. Taylor C. W. Time and space--novel aspects of hormone action. Trends Pharmacol Sci. 1988 Feb;9(2):43–45. doi: 10.1016/0165-6147(88)90111-3. [DOI] [PubMed] [Google Scholar]
  14. Woodcock E. A., White L. B., Smith A. I., McLeod J. K. Stimulation of phosphatidylinositol metabolism in the isolated, perfused rat heart. Circ Res. 1987 Nov;61(5):625–631. doi: 10.1161/01.res.61.5.625. [DOI] [PubMed] [Google Scholar]

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