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. 1989 Jul 1;261(1):305–308. doi: 10.1042/bj2610305

Human inter-alpha-trypsin inhibitor. Synthesis and maturation in hepatoma HepG2 cells.

J Bourguignon 1, R Sesboüé 1, M Diarra-Mehrpour 1, M Daveau 1, J P Martin 1
PMCID: PMC1138820  PMID: 2476114

Abstract

In hepatoma HepG2 cells, human inter-alpha-trypsin inhibitor (ITI) was synthesized as three heavy chains, H-1 (100 kDa), H-2 (110 kDa) and H-3 (113 kDa), and light hybrid chain (49.5 kDa) composed of alpha 1-microglobulin and HI-30 (ITI derivative, human inhibitor of 30 kDa). The association of at least two heavy chains, H-1 and H-3, with the HI-30 part of the light chain gave rise to a molecule similar to serum ITI. A composite protein (approximately 250 kDa) including heavy and light chains was also secreted, while alpha 1-microglobulin and ITI H-2 protein were released as separate entities. Light chain synthesis could be the limiting factor for ITI maturation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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