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. 1989 Aug 1;261(3):739–742. doi: 10.1042/bj2610739

The rate of the AMP/adenosine substrate cycle in concanavalin-A-stimulated rat lymphocytes.

Z Szondy 1, E A Newsholme 1
PMCID: PMC1138893  PMID: 2552990

Abstract

The effect of adenosine on the metabolism of prelabelled adenine nucleotides was investigated in concanavalin-A-stimulated rat lymphocytes. Adenosine in the presence of the adenosine deaminase inhibitor, deoxycoformycin, caused a 2-fold increase in the ATP concentration. This effect was, in part, countereacted by an increased rate of adenine nucleotide catabolism, which could be explained by a stimulation of AMP deaminase (EC 3.5.4.6). At the same time a continuous rate of labelled adenosine production was found, which was not affected by the increased ATP concentration and which could only be detected by the trapping effect of a high concentration of added unlabelled adenosine. It is concluded that the rate of the substrate cycle between AMP and adenosine is low (1.9 +/- 0.2 nmol/h per 10(7) cells) in comparison to the rate of AMP deamination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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