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. 1989 Aug 1;261(3):787–792. doi: 10.1042/bj2610787

Low-Mr iron isolated from guinea pig reticulocytes as AMP-Fe and ATP-Fe complexes.

J Weaver 1, S Pollack 1
PMCID: PMC1138900  PMID: 2803243

Abstract

Guinea pig reticulocytes were pulse-labelled with 59Fe bound to transferrin. Haemolysates prepared from these reticulocytes were subjected to rapid (NH1)2SO1 precipitation and then chromatography on an anion-exchange resin. ATP-bound 59Fe was the dominant species in the reticulocyte cytosol; 2,3-bisphosphoglycerate and GTP iron complexes were not detected despite the fact that these were stable with (NH1)2SO1 precipitation and readily detected with anion-exchange chromatography. AMP-bound Fe was a minor component of the cytosol following rapid (NH1)2SO4 precipitation, and the major component when iron was released from transferrin by haemolysates. We speculate that ATP-Fe may be degraded in the cell to permit utilization of its iron for haem synthesis.

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Selected References

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