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. 2024 Aug 9;15(9):e00781-24. doi: 10.1128/mbio.00781-24

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Protein sequence alignment depicts conserved regions and residues. Scatterplots compare fitness of genes of interest across all conditions, with a focus on autotrophic growth conditions. — Cofitness between 4Fe-4S encoding homologs is specific to PorF and the CODH-ACS 4Fe-4S encoded by MMP_RS05070. (A) Alignment of 4Fe-FS encoding MMP_RS05070, MMP_RS07720, and MMP_RS07725 with the two conserved 4Fe-4S motifs (CXXCXXCXXXC) labeled. Amino acids are colored using the Clustal X color scheme. (B) Cofitness of MMP_RS05070 and MMP_RS07720 (PorF). (C) Negative cofitness of MMP_RS05070 and MMP_RS07725 (PorE). (D) Negative cofitness of MMP_RS07725 (PorE) and MMP_RS07720 (PorF). Experiments identified as autotrophic conditions were performed in defined minimal media both with and without vitamins and either H₂ or formate as the electron donor.