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PLS3 knockdown in MLO-Y4 cells. (A) PLS3, ACTN1, and ACTN4 expression determined by Western blotting analysis from day 1 to 7 after the transfection. TUBA4A was used as a loading control. (B) Pls3 mRNA expression after 24 h of transfection. Tbp was used to normalize gene expression. si-Pls3: small interfering Pls3. (Error bars indicate standard deviation, **** p < 0.0001).
