Table 2.
Primers used for real-time qPCR.
| Gene | Accession Number | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
|---|---|---|---|
| PLS3 | NM_005032.4 | CTCCCTGGTTGGCATTGGAG | GCCAAACTGGAGCTGATCGT |
| ACTN1 | NM_001130004.1 | CCCGAGCTGATTGACTACGG | ATGGCTTTCTCATCCGGTCG |
| ACTN4 | NM_004924.5 | GGCACAGACCAGAGCTGATT | AAGCGTGGTAGAAGCAGGAC |
| FSCN1 | NM_003088.3 | GTCGACTCGCTCATCACCCTC | GGAAGGTCTCCTGGTCGGTC |
| RUNX2 | NM_001024630.3 | ACTGCTTGCAGCCTTAAAT | ATGCTTCATTCGCCTCAC |
| ALP | NM_000478.5 | AGGGACATTGACGTGATCAT | CCTGGCTCGAAGAGACC |
| OPN | NM_001040058.1 | TTCCAAGTAAGTCCAACGAAAG | GTGACCAGTTCATCAGATTCAT |
| SFRP1 | NM_003013.2 | CGTGGGCTACAAGAAGATGG | AAGCCGAAGAACTGCATGAC |
| WNT1 | NM_005430.3 | GCCGATGGTGGGGTATTGTG | GATCCCCGGATTTTGGCGTA |
| CTNND2 | NM_001332.3 | CCGGGTGCCTAAGGAATGTT | CGCAGACTGGATCACGTACA |
| WNT2 | NM_003391.2 | CATCCAGATGTGATGCGTGC | GCAGATTCCCGACTACTTCG |
| NFATC2 | NM_012340.4 | CATGAGGGCAACCATCGACT | CCCCGTGAGGATCATTTGCT |
| NFATC1 | NM_006162.4 | AACCCCATCGAATGCTCCCAG | AGAAACTGACGTGAACGGGG |
| FZD3 | NM_017412.3 | CGCCGGGGTCTGAGATATT | GCTTGGTCTCACAAAAGGCG |
| GAPDH | NM_002046.6 | GCCCAATACGACCAAATCC | AGCCACATCGCTCAGACAC |
| Pls3 | NM_145629.1 | CAAGCACAATAACGCCAAGTACGC | ACTCTCTTCATCCCTCTGCCCATC |
| Tbp | NM_013684.2 | TGGCGGTTTGGCTAGGTTTC | CACCATGAAATAGTGATGCTGGG |