Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1987 Feb;40(2):143–146. doi: 10.1136/jcp.40.2.143

Immunohistological detection of Legionella pneumophila in lung sections.

J M Theaker, J O Tobin, S E Jones, P Kirkpatrick, M I Vina, K A Fleming
PMCID: PMC1140856  PMID: 3546392

Abstract

Immunohistology was used for the detection of Legionella pneumophila serogroup I in necropsy tissue. Study of pneumonic lung from the recent Stafford outbreak has shown that this technique has a high sensitivity. A retrospective postmortem examination showed that L pneumophila serogroup 1 was an unusual cause of pneumonia in Oxfordshire during the study period. L pneumophila serogroup 1 can be successfully subgrouped, using a panel of monoclonal antibodies on formalin fixed paraffin embedded sections. Immunohistological methods have a potentially useful role in the diagnosis of Legionellosis at postmortem examination and in the epidemiological investigation of individual cases and outbreaks.

Full text

PDF
143

Images in this article

144Fig 1
on p.144
144Figs 2 and 3
on p.144

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blackmon J. A., Chandler F. W., Cherry W. B., England A. C., 3rd, Feeley J. C., Hicklin M. D., McKinney R. M., Wilkinson H. W. Legionellosis. Am J Pathol. 1981 Jun;103(3):429–465. [PMC free article] [PubMed] [Google Scholar]
  2. Burns J. Immunoperoxidase localisation of hepatitis B antigen (HB) in formalin-paraffin processed liver tissue. Histochemistry. 1975 Jul 30;44(2):133–135. doi: 10.1007/BF00494074. [DOI] [PubMed] [Google Scholar]
  3. Cherry W. B., Pittman B., Harris P. P., Hebert G. A., Thomason B. M., Thacker L., Weaver R. E. Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J Clin Microbiol. 1978 Sep;8(3):329–338. doi: 10.1128/jcm.8.3.329-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelstein P. H., Meyer R. D., Finegold S. M. Laboratory diagnosis of Legionnaires' disease. Am Rev Respir Dis. 1980 Feb;121(2):317–327. doi: 10.1164/arrd.1980.121.2.317. [DOI] [PubMed] [Google Scholar]
  5. Joly J. R., McKinney R. M., Tobin J. O., Bibb W. F., Watkins I. D., Ramsay D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol. 1986 Apr;23(4):768–771. doi: 10.1128/jcm.23.4.768-771.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Reingold A. L., Thomason B. M., Brake B. J., Thacker L., Wilkinson H. W., Kuritsky J. N. Legionella pneumonia in the United States: the distribution of serogroups and species causing human illness. J Infect Dis. 1984 May;149(5):819–819. doi: 10.1093/infdis/149.5.819. [DOI] [PubMed] [Google Scholar]
  7. Watkins I. D., Tobin J. O., Dennis P. J., Brown W., Newnham R., Kurtz J. B. Legionella pneumophila serogroup 1 subgrouping by monoclonal antibodies--an epidemiological tool. J Hyg (Lond) 1985 Oct;95(2):211–216. doi: 10.1017/s0022172400062641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Woodruff J. D., Braun L., Cavalieri R., Gupta P., Pass F., Shah K. V. Immunologic identification of papillomavirus antigen in condyloma tissues from the female genital tract. Obstet Gynecol. 1980 Dec;56(6):727–732. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES