Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1987 Feb;40(2):157–162. doi: 10.1136/jcp.40.2.157

Immunohistochemical staining of colorectal tissues with monoclonal antibodies to ras oncogene p21 product and carbohydrate determinant antigen 19-9.

D C Allen, H Foster, J C Orchin, J D Biggart
PMCID: PMC1140859  PMID: 3546394

Abstract

Two monoclonal antibodies were applied to benign, dysplastic, and malignant human colorectal tissues using immunohistochemical techniques on formalin fixed paraffin embedded material. RAP-5 antibody is directed against a synthetic peptide, reflecting an amino acid sequence of the ras oncogene p21 protein product. Despite using several different techniques and antibody dilutions differential staining between the various epithelial populations was not obtained. RAP-5 also showed other tissue components such as plasma cells, histiocytes, fibroblasts, smooth muscle and vascular endothelium. CA19-9 antibody recognizes an epithelial surface carbohydrate antigen originally derived from a human colorectal carcinoma cell line: it did not stain normal colorectal mucosa or adenomatous polyps, but showed focal expression of variable strength in regenerative, dysplastic, and cancerous mucosa in ulcerative colitis, and in non-colitic colorectal carcinoma. Neither antibody was found to be a reliable marker of the evolution of malignant mucosal changes, although CA19-9 may be of limited use in confirming adenocarcinoma of gastrointestinal origin.

Full text

PDF
157

Images in this article

158Fig 1
on p.158
159Fig 2
on p.159
160Fig 3
on p.160
161Fig 4
on p.161

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen D. C., Biggart J. D., Orchin J. C., Foster H. An immunoperoxidase study of epithelial marker antigens in ulcerative colitis with dysplasia and carcinoma. J Clin Pathol. 1985 Jan;38(1):18–29. doi: 10.1136/jcp.38.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allen D. C., Biggart J. D., Pyper P. C. Large bowel mucosal dysplasia and carcinoma in ulcerative colitis. J Clin Pathol. 1985 Jan;38(1):30–43. doi: 10.1136/jcp.38.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Atkinson B. F., Ernst C. S., Herlyn M., Steplewski Z., Sears H. F., Koprowski H. Gastrointestinal cancer-associated antigen in immunoperoxidase assay. Cancer Res. 1982 Nov;42(11):4820–4823. [PubMed] [Google Scholar]
  4. Bishop J. M. Viruses, genes, and cancer. II. Retroviruses and cancer genes. Cancer. 1985 May 15;55(10):2329–2333. doi: 10.1002/1097-0142(19850515)55:10<2329::aid-cncr2820551005>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  5. Del Villano B. C., Brennan S., Brock P., Bucher C., Liu V., McClure M., Rake B., Space S., Westrick B., Schoemaker H. Radioimmunometric assay for a monoclonal antibody-defined tumor marker, CA 19-9. Clin Chem. 1983 Mar;29(3):549–552. [PubMed] [Google Scholar]
  6. GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ghosh A. K., Moore M., Harris M. Immunohistochemical detection of ras oncogene p21 product in benign and malignant mammary tissue in man. J Clin Pathol. 1986 Apr;39(4):428–434. doi: 10.1136/jcp.39.4.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hand P. H., Thor A., Wunderlich D., Muraro R., Caruso A., Schlom J. Monoclonal antibodies of predefined specificity detect activated ras gene expression in human mammary and colon carcinomas. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5227–5231. doi: 10.1073/pnas.81.16.5227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heyderman E., Steele K., Ormerod M. G. A new antigen on the epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue. J Clin Pathol. 1979 Jan;32(1):35–39. doi: 10.1136/jcp.32.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jalanko H., Kuusela P., Roberts P., Sipponen P., Haglund C. A., Mäkelä O. Comparison of a new tumour marker, CA 19-9, with alpha-fetoprotein and carcinoembryonic antigen in patients with upper gastrointestinal diseases. J Clin Pathol. 1984 Feb;37(2):218–222. doi: 10.1136/jcp.37.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kerr I. B., Lee F. D., Quintanilla M., Balmain A. Immunocytochemical demonstration of p21 ras family oncogene product in normal mucosa and in premalignant and malignant tumours of the colorectum. Br J Cancer. 1985 Nov;52(5):695–700. doi: 10.1038/bjc.1985.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Konishi F., Morson B. C. Pathology of colorectal adenomas: a colonoscopic survey. J Clin Pathol. 1982 Aug;35(8):830–841. doi: 10.1136/jcp.35.8.830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Koprowski H., Steplewski Z., Mitchell K., Herlyn M., Herlyn D., Fuhrer P. Colorectal carcinoma antigens detected by hybridoma antibodies. Somatic Cell Genet. 1979 Nov;5(6):957–971. doi: 10.1007/BF01542654. [DOI] [PubMed] [Google Scholar]
  14. Lehy T., Mignon M., Abitbol J. L. Epithelial cell proliferation in the rectal stump of patients with ileorectal anastomosis for ulcerative colitis. Gut. 1983 Nov;24(11):1048–1056. doi: 10.1136/gut.24.11.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Leibovitz A., Stinson J. C., McCombs W. B., 3rd, McCoy C. E., Mazur K. C., Mabry N. D. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 1976 Dec;36(12):4562–4569. [PubMed] [Google Scholar]
  16. Magnani J. L., Nilsson B., Brockhaus M., Zopf D., Steplewski Z., Koprowski H., Ginsburg V. A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II. J Biol Chem. 1982 Dec 10;257(23):14365–14369. [PubMed] [Google Scholar]
  17. McCarthy N. C., Simpson J. R., Coghill G., Kerr M. A. Expression in normal adult, fetal, and neoplastic tissues of a carbohydrate differentiation antigen recognised by antigranulocyte mouse monoclonal antibodies. J Clin Pathol. 1985 May;38(5):521–529. doi: 10.1136/jcp.38.5.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McGrath J. P., Capon D. J., Goeddel D. V., Levinson A. D. Comparative biochemical properties of normal and activated human ras p21 protein. Nature. 1984 Aug 23;310(5979):644–649. doi: 10.1038/310644a0. [DOI] [PubMed] [Google Scholar]
  19. Nakane P K, Pierce G B., Jr Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966 Dec;14(12):929–931. doi: 10.1177/14.12.929. [DOI] [PubMed] [Google Scholar]
  20. Parada L. F., Tabin C. J., Shih C., Weinberg R. A. Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene. Nature. 1982 Jun 10;297(5866):474–478. doi: 10.1038/297474a0. [DOI] [PubMed] [Google Scholar]
  21. Paul J. Oncogenes. J Pathol. 1984 May;143(1):1–10. doi: 10.1002/path.1711430103. [DOI] [PubMed] [Google Scholar]
  22. Poger M. E., Lamm M. E. Localization of free and bound secretory component in human intestinal epithelial cells. A model for the assembly of secretory IgA. J Exp Med. 1974 Mar 1;139(3):629–642. doi: 10.1084/jem.139.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pulciani S., Santos E., Lauver A. V., Long L. K., Aaronson S. A., Barbacid M. Oncogenes in solid human tumours. Nature. 1982 Dec 9;300(5892):539–542. doi: 10.1038/300539a0. [DOI] [PubMed] [Google Scholar]
  24. Riddell R. H., Goldman H., Ransohoff D. F., Appelman H. D., Fenoglio C. M., Haggitt R. C., Ahren C., Correa P., Hamilton S. R., Morson B. C. Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications. Hum Pathol. 1983 Nov;14(11):931–968. doi: 10.1016/s0046-8177(83)80175-0. [DOI] [PubMed] [Google Scholar]
  25. Slamon D. J., deKernion J. B., Verma I. M., Cline M. J. Expression of cellular oncogenes in human malignancies. Science. 1984 Apr 20;224(4646):256–262. doi: 10.1126/science.6538699. [DOI] [PubMed] [Google Scholar]
  26. Spandidos D. A., Kerr I. B. Elevated expression of the human ras oncogene family in premalignant and malignant tumours of the colorectum. Br J Cancer. 1984 Jun;49(6):681–688. doi: 10.1038/bjc.1984.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thor A., Horan Hand P., Wunderlich D., Caruso A., Muraro R., Schlom J. Monoclonal antibodies define differential ras gene expression in malignant and benign colonic diseases. Nature. 1984 Oct 11;311(5986):562–565. doi: 10.1038/311562a0. [DOI] [PubMed] [Google Scholar]
  28. Wagener C., Hain F., Breuer H., Olude S., Cremer H., Müller-Wallraf R. Immunohistochemical demonstration of carcinoembryonic antigen in normal, transitional and inflamed colonic mucosa. Oncodev Biol Med. 1981;2(5):331–343. [PubMed] [Google Scholar]
  29. Williams A. R., Piris J., Spandidos D. A., Wyllie A. H. Immunohistochemical detection of the ras oncogene p21 product in an experimental tumour and in human colorectal neoplasms. Br J Cancer. 1985 Nov;52(5):687–693. doi: 10.1038/bjc.1985.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wolf B., Thompson J., Von Kleist S. Ultrastructural localization of carcino-embryonic antigen in a continuous human tumour cell line (LS 174 T) in relation to morphometric investigations. Anticancer Res. 1984 Jul-Oct;4(4-5):213–219. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES