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. 1987 Jun;40(6):608–611. doi: 10.1136/jcp.40.6.608

Colorectal goblet cell mucins in familial adenomatous polyposis.

K Sugihara, J R Jass
PMCID: PMC1141047  PMID: 3038964

Abstract

Ninety two tissue blocks from the left colon and 52 from the right colon were obtained from 112 patients with familial adenomatous polyposis (FAP). Tissues from 137 patients with other conditions served as controls. Within the main study a smaller investigation was performed to compare sections from the left and right colon in the same subject. Several well known histochemical techniques were used to investigate possible changes in sulphation, sialic acid structure (loss of O-acetyl substituents), and changes in the ratio of sialic acid to neutral sugars. In patients with FAP, as in controls, there was increased expression of periodic acid Schiff positive mucin and fucose in the right colon. The only difference between patients with FAP and controls was the indirect demonstration of less neutral mucin in the right colon in FAP, but this did not seem to affect neutral sugars binding to UEA-1, PNL, or HPA. As in the general population, a small proportion of patients with FAP showed a lack of O-acetyl substituted sialic acid. Sialic acid heterogeneity probably has a genetic basis, but this is not associated with the genetic defect underlying FAP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Culling C. F., Reid P. E., Dunn W. L. The effect of saponification upon certain histochemical reactions of the epithelial mucins of the gastrointestinal tract. J Histochem Cytochem. 1971 Nov;19(11):654–662. doi: 10.1177/19.11.654. [DOI] [PubMed] [Google Scholar]
  2. Culling C. F., Reid P. E., Worth A. J., Dunn W. L. A new histochemical technique of use in the interpretation and diagnosis of adenocarcinoma and villous lesions in the large intestine. J Clin Pathol. 1977 Nov;30(11):1056–1062. doi: 10.1136/jcp.30.11.1056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Filipe M. I., Branfoot A. C. Abnormal patterns of mucus secretion in apparently normal mucosa of large intestine with carcinoma. Cancer. 1974 Aug;34(2):282–290. doi: 10.1002/1097-0142(197408)34:2<282::aid-cncr2820340211>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  4. Filipe M. I., Mughal S., Bussey H. J. Patterns of mucus secretion in the colonic epithelium in familial polyposis. Invest Cell Pathol. 1980 Oct-Dec;3(4):329–343. [PubMed] [Google Scholar]
  5. Knudson A. G., Jr Mutation and cancer: statistical study of retinoblastoma. Proc Natl Acad Sci U S A. 1971 Apr;68(4):820–823. doi: 10.1073/pnas.68.4.820. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lev R., Lance P., Camara P. Histochemical and morphologic studies of mucosa bordering rectosigmoid carcinomas: comparisons with normal, diseased, and malignant colonic epithelium. Hum Pathol. 1985 Feb;16(2):151–161. doi: 10.1016/s0046-8177(85)80064-2. [DOI] [PubMed] [Google Scholar]
  7. Matsumoto I., Osawa T. Purification and characterization of an anti-H(O) phytohemagglutinin of Ulex europeus. Biochim Biophys Acta. 1969 Nov 11;194(1):180–189. doi: 10.1016/0005-2795(69)90193-7. [DOI] [PubMed] [Google Scholar]
  8. Muto T., Kamiya J., Sawada T., Agawa S., Morioka Y., Utsunomiya J. Mucin abnormality of colonic mucosa in patients with familial polyposis coli. A new tool for early detection of the carrier? Dis Colon Rectum. 1985 Mar;28(3):147–148. doi: 10.1007/BF02554225. [DOI] [PubMed] [Google Scholar]
  9. Picard J. K., Feizi T. Peanut lectin and anti-Ii antibodies reveal structural differences among human gastrointestinal glycoproteins. Mol Immunol. 1983 Nov;20(11):1215–1220. doi: 10.1016/0161-5890(83)90145-1. [DOI] [PubMed] [Google Scholar]
  10. Reid P. E., Culling C. F., Dunn W. L., Ramey C. W., Clay M. G. Chemical and histochemical studies of normal and diseased human gastrointestinal tract. I. A comparison between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon. Histochem J. 1984 Mar;16(3):235–251. doi: 10.1007/BF01003608. [DOI] [PubMed] [Google Scholar]
  11. Reid P. E., Owen D. A., Dunn W. L., Ramey C. W., Lazosky D. A., Clay M. G. Chemical and histochemical studies of normal and diseased human gastrointestinal tract. III. Changes in the histochemical and chemical properties of the epithelial glycoproteins in the mucosa close to colonic tumours. Histochem J. 1985 Feb;17(2):171–181. doi: 10.1007/BF01003216. [DOI] [PubMed] [Google Scholar]
  12. SPICER S. S. The use of various cationic reagents in histochemical differentiation of mucopolysaccharides. Am J Clin Pathol. 1961 Nov;36:393–407. doi: 10.1093/ajcp/36.5.393. [DOI] [PubMed] [Google Scholar]
  13. Yonezawa S., Nakamura T., Tanaka S., Maruta K., Nishi M., Sato E. Binding of Ulex europaeus agglutinin-I in polyposis coli: comparative study with solitary adenoma in the sigmoid colon and rectum. J Natl Cancer Inst. 1983 Jul;71(1):19–24. [PubMed] [Google Scholar]
  14. Yonezawa S., Nakamura T., Tanaka S., Sato E. Glycoconjugate with Ulex europaeus agglutinin-I-binding sites in normal mucosa, adenoma, and carcinoma of the human large bowel. J Natl Cancer Inst. 1982 Oct;69(4):777–785. [PubMed] [Google Scholar]

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