Abstract
High concentrations of circulating immune complexes were detected by polyethylene glycol precipitation in 11 of 20 patients with myelofibrosis secondary to chronic myeloproliferative disease. Circulating immune complexes showed a positive correlation with plasma IgG concentrations both in patients and controls. Covariance analysis of the two groups showed significantly increased polyethylene glycol precipitable IgG in patients when adjusted for plasma IgG concentrations, indicating that the patients had significantly increased concentrations of complexed IgG. The immune complexes contained IgG, C3, and fibronectin and were inversely correlated with plasma fibronectin concentrations, suggesting that this major non-specific opsonin is important for the normal clearance of immune complexes. Therapeutic plasmapheresis efficiently removed circulating complexes and produced an increase in plasma fibronectin. This suggests that plasmapheresis may be useful for controlling immune complex mediated complications of these disorders.
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Selected References
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- Cappio F. C., Vigliani R., Novarino A., Camussi G., Campana D., Gavosto F. Idiopathic myelofibrosis: a possible role for immune-complexes in the pathogenesis of bone marrow fibrosis. Br J Haematol. 1981 Sep;49(1):17–21. doi: 10.1111/j.1365-2141.1981.tb07192.x. [DOI] [PubMed] [Google Scholar]
- Creighton W. D., Lambert P. H., Miescher P. A. Detection of antibodies and soluble antigen-antibody complexes by precipitation with polyethylene glycol. J Immunol. 1973 Oct;111(4):1219–1227. [PubMed] [Google Scholar]
- Gordon B. R., Coleman M., Kohen P., Day N. K. Immunologic abnormalities in myelofibrosis with activation of the complement system. Blood. 1981 Nov;58(5):904–910. [PubMed] [Google Scholar]
- Lewis C. M., Pegrum G. D. Immune complexes in myelofibrosis: a possible guide to management. Br J Haematol. 1978 Jun;39(2):233–239. doi: 10.1111/j.1365-2141.1978.tb01093.x. [DOI] [PubMed] [Google Scholar]
- Mosesson M. W., Umfleet R. A. The cold-insoluble globulin of human plasma. I. Purification, primary characterization, and relationship to fibrinogen and other cold-insoluble fraction components. J Biol Chem. 1970 Nov 10;245(21):5728–5736. [PubMed] [Google Scholar]
- Norfolk D. R., Bowen M., Roberts B. E., Child J. A. Plasma fibronectin in myeloproliferative disorders and chronic granulocytic leukaemia. Br J Haematol. 1983 Oct;55(2):319–324. doi: 10.1111/j.1365-2141.1983.tb01253.x. [DOI] [PubMed] [Google Scholar]
- Simpson A. W., Boughton B. J. Fibronectin as an opsonic regulator of monocyte phagocytosis. J Clin Pathol. 1984 Jul;37(7):787–789. doi: 10.1136/jcp.37.7.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vellenga E., Mulder N. H., The T. H., Nieweg H. O. A study of the cellular and humoral immune response in patients with myelofibrosis. Clin Lab Haematol. 1982;4(3):239–246. doi: 10.1111/j.1365-2257.1982.tb00073.x. [DOI] [PubMed] [Google Scholar]
- Vuento M., Vaheri A. Purification of fibronectin from human plasma by affinity chromatography under non-denaturing conditions. Biochem J. 1979 Nov 1;183(2):331–337. doi: 10.1042/bj1830331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yuan L., Campbell D. H. The in vivo and in vitro immunogenicity and antigenic specificity of lymphoid antigen fragment-oligoribonucleopeptide conjugates. Immunochemistry. 1972 Jan;9(1):1–8. doi: 10.1016/0019-2791(72)90277-7. [DOI] [PubMed] [Google Scholar]