Figure 5.

Overview of the Tier system. (a) Schematic showing how provisional and final tiers can be assigned to ncORFs. First Ribo-seq, proteomics and immunopeptidomics data can be (computationally) integrated to assign provisional tiers based on the quality of each data entity. Manual inspection of each data entity is then necessary to assign a final tier to each ncORF. In this figure, ‘+’ denotes detection, ‘++’ denotes abundant detection, ‘+/−’ denotes either presence or absence of detection, and ‘−’ denotes absence of detection. (b) Results of the provisional and final tier assignment for the 7,264 ncORFs analyzed for this study. (c) Overview of the curation process for the provisional Tier 1A ncORFs.