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. 1989 Apr;42(4):414–421. doi: 10.1136/jcp.42.4.414

KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.

K A Pulford 1, E M Rigney 1, K J Micklem 1, M Jones 1, W P Stross 1, K C Gatter 1, D Y Mason 1
PMCID: PMC1141915  PMID: 2654191

Abstract

A new monoclonal antibody, KP1, raised against a lysosomal fraction of human lung macrophages, recognises a fixation-resistant epitope in a wide variety of tissue macrophages (such as Kupffer cells germinal centre, splenic, and lamina propria macrophages), and in granulocyte precursors. Its broad reactivity with cells of the mononuclear phagocytic lineage was established by testing on routinely processed samples of normal and reactive lymphoid tissues. Interdigitating reticulum cells were unstained or showed limited cytoplasmic staining while Langerhans' cells and follicular dendritic reticulum cells were unreactive. KP1 recognises a molecule of about 110 kilodaltons in macrophage-rich human tissue when tested by either immunoprecipitation or Western blotting (although the latter procedure also shows two additional components with molecular weights of 70 and 40 kilodaltons). KP1 should be of considerable value for studying disorders of the monocyte/macrophage system, including both reactive and neoplastic states (such as true histiocytic proliferations).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M. Lymphoid dendritic cells. Immunology. 1987 Oct;62(2):161–170. [PMC free article] [PubMed] [Google Scholar]
  2. Cline M. J., Lehrer R. I., Territo M. C., Golde D. W. UCLA Conference. Monocytes and macrophages: functions and diseases. Ann Intern Med. 1978 Jan;88(1):78–88. doi: 10.7326/0003-4819-88-1-78. [DOI] [PubMed] [Google Scholar]
  3. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  4. Davey F. R., Cordell J. L., Erber W. N., Pulford K. A., Gatter K. C., Mason D. Y. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988 Jul;41(7):753–758. doi: 10.1136/jcp.41.7.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davey F. R., Gatter K. C., Ralfkiaer E., Pulford K. A., Krissansen G. W., Mason D. Y. Immunophenotyping of non-Hodgkin's lymphomas using a panel of antibodies on paraffin-embedded tissues. Am J Pathol. 1987 Oct;129(1):54–63. [PMC free article] [PubMed] [Google Scholar]
  6. Erber W. N., Mason D. Y. Immunoalkaline phosphatase labeling of terminal transferase in hematologic samples. Am J Clin Pathol. 1987 Jul;88(1):43–50. doi: 10.1093/ajcp/88.1.43. [DOI] [PubMed] [Google Scholar]
  7. Facchetti F., de Wolf-Peeters C., Mason D. Y., Pulford K., van den Oord J. J., Desmet V. J. Plasmacytoid T cells. Immunohistochemical evidence for their monocyte/macrophage origin. Am J Pathol. 1988 Oct;133(1):15–21. [PMC free article] [PubMed] [Google Scholar]
  8. Fearon D. T., Collins L. A. Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J Immunol. 1983 Jan;130(1):370–375. [PubMed] [Google Scholar]
  9. Flavell D. J., Jones D. B., Wright D. H. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem. 1987 Nov;35(11):1217–1226. doi: 10.1177/35.11.3309045. [DOI] [PubMed] [Google Scholar]
  10. Franklin W. A., Mason D. Y., Pulford K., Falini B., Bliss E., Gatter K. C., Stein H., Clarke L. C., McGee J. O. Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest. 1986 Mar;54(3):322–335. [PubMed] [Google Scholar]
  11. Hogg N., MacDonald S., Slusarenko M., Beverley P. C. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology. 1984 Dec;53(4):753–767. [PMC free article] [PubMed] [Google Scholar]
  12. Hogg N., Selvendran Y. An anti-human monocyte/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue. Cell Immunol. 1985 May;92(2):247–253. doi: 10.1016/0008-8749(85)90006-1. [DOI] [PubMed] [Google Scholar]
  13. Johnston R. B., Jr Current concepts: immunology. Monocytes and macrophages. N Engl J Med. 1988 Mar 24;318(12):747–752. doi: 10.1056/NEJM198803243181205. [DOI] [PubMed] [Google Scholar]
  14. Kelly P. M., Bliss E., Morton J. A., Burns J., McGee J. O. Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol. 1988 May;41(5):510–515. doi: 10.1136/jcp.41.5.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Parwaresch M. R., Radzun H. J., Kreipe H., Hansmann M. L., Barth J. Monocyte/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen. Am J Pathol. 1986 Oct;125(1):141–151. [PMC free article] [PubMed] [Google Scholar]
  17. Radzun H. J., Parwaresch M. R. Differential immunohistochemical resolution of the human mononuclear phagocyte system. Cell Immunol. 1983 Nov;82(1):174–183. doi: 10.1016/0008-8749(83)90151-x. [DOI] [PubMed] [Google Scholar]
  18. Radzun H. J., Parwaresch M. R., Feller A. C., Hansmann M. L. Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells. Am J Pathol. 1984 Dec;117(3):441–450. [PMC free article] [PubMed] [Google Scholar]
  19. Schuler G., Steinman R. M. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J Exp Med. 1985 Mar 1;161(3):526–546. doi: 10.1084/jem.161.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Steinman R. M., Nussenzweig M. C. Dendritic cells: features and functions. Immunol Rev. 1980;53:127–147. doi: 10.1111/j.1600-065x.1980.tb01042.x. [DOI] [PubMed] [Google Scholar]
  21. Tew J. G., Thorbecke G. J., Steinman R. M. Dendritic cells in the immune response: characteristics and recommended nomenclature (A report from the Reticuloendothelial Society Committee on Nomenclature). J Reticuloendothel Soc. 1982 May;31(5):371–380. [PubMed] [Google Scholar]
  22. Todd R. F., 3rd, Roach J. A., Arnaout M. A. The modulated expression of Mo5, a human myelomonocytic plasma membrane antigen. Blood. 1985 Apr;65(4):964–973. [PubMed] [Google Scholar]
  23. Van Voorhis W. C., Steinman R. M., Hair L. S., Luban J., Witmer M. D., Koide S., Cohn Z. A. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J Exp Med. 1983 Jul 1;158(1):126–145. doi: 10.1084/jem.158.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Woodcock-Mitchell J., Eichner R., Nelson W. G., Sun T. T. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580–588. doi: 10.1083/jcb.95.2.580. [DOI] [PMC free article] [PubMed] [Google Scholar]

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