Abstract
A system of automatic jar evacuation-replacement (Anoxomat) for the culture of anaerobes, capnophiles, and microaerophiles was compared with existing methods of anaerobic cabinets, carbon dioxide incubators, and manual evacuation-replacement. Of the 50 species of anaerobes, 29 strains of capnophiles, and 11 strains of microaerophiles tested, equivalent growth was obtained in all but two instances. The Anoxomat system yielded slightly larger colonies in 26 (52%) of anaerobes tested with superior growth in the anerobic cabinets in three (6%) of cases and equal in both in 21 (42%). Of the microaerophiles and capnophiles tested, there was no significant difference between the Anoxomat and the conventional system. The Anoxomat system seems to be a suitable alternative to anaerobic and carbon dioxide incubators.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arank A., Syed S. A., Kenney E. B., Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. doi: 10.1128/am.17.4.568-576.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berry P. L., Taylor E., Phillips I. The use of an anaerobic incubator for the isolation of anaerobes from clinical samples. J Clin Pathol. 1982 Oct;35(10):1158–1162. doi: 10.1136/jcp.35.10.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drasar B. S. Cultivation of anaerobic intestinal bacteria. J Pathol Bacteriol. 1967 Oct;94(2):417–427. doi: 10.1002/path.1700940223. [DOI] [PubMed] [Google Scholar]
- ROSEBURY T., REYNOLDS J. B. CONTINUOUS ANAEROBIOSIS FOR CULTIVATION OF SPIROCHETES. Proc Soc Exp Biol Med. 1964 Dec;117:813–815. doi: 10.3181/00379727-117-29706. [DOI] [PubMed] [Google Scholar]
- Watt B., Collee J. G., Brown R. The isolation of strict anaerobes: the use of an anaerobic cabinet compared with a conventional procedure. J Med Microbiol. 1974 Aug;7(3):315–324. doi: 10.1099/00222615-7-3-315. [DOI] [PubMed] [Google Scholar]