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. 1989 Jul;42(7):689–693. doi: 10.1136/jcp.42.7.689

Hepatitis B-DNA replication and histological patterns in liver biopsy specimens of chronic HBsAg positive patients with and without hepatitis delta virus superinfection.

D Villari 1, G Raimondo 1, V Smedile 1, G Rodinó 1, S Brancatelli 1, G Longo 1, G Squadrito 1, D Batolo 1
PMCID: PMC1142015  PMID: 2760230

Abstract

The role of active hepatitis B virus (HBV) infection in chronic HBsAg positive hepatitis with and without hepatitis delta virus (HDV) superinfection was analysed in percutaneous liver biopsy specimens from 50 patients. Each specimen was divided into two--one part for histological evaluation and for the detection of HBcAg and delta antigen; the other part was tested for HBV-DNA using Southern blotting. Ten cases were of chronic lobular hepatitis, 10 of chronic persistent hepatitis, and 30 of chronic active hepatitis. Ten cases were delta antigen positive and showed high grade lobular activity but no evidence of HBV-DNA episomal forms or HBcAg reactivity. Twenty one cases showed HBV-DNA replicative intermediate forms; 19 had high grade lobular activity, which occurred in five cases without evidence of free viral DNA. Of the 21 biopsy specimens with HBV-DNA episomal forms, 14 were positive for HBcAg; only one of the 19 cases without detectable viral DNA was positive for such antigen. These data indicate that the presence of HBV or HDV active infection correlates with the histological finding of prominent lobular necrosis. Moreover, intrahepatic HBV-DNA seems to be a more sensitive marker than the presence of viral antigens for indicating HBV replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonino F., Rosina F., Rizzetto M., Rizzi R., Chiaberge E., Tardanico R., Callea F., Verme G. Chronic hepatitis in HBsAg carriers with serum HBV-DNA and anti-HBe. Gastroenterology. 1986 May;90(5 Pt 1):1268–1273. doi: 10.1016/0016-5085(86)90395-1. [DOI] [PubMed] [Google Scholar]
  2. Bonino F. The importance of hepatitis B viral DNA in serum and liver. J Hepatol. 1986;3(1):136–141. doi: 10.1016/s0168-8278(86)80158-1. [DOI] [PubMed] [Google Scholar]
  3. Brechot C., Hadchouel M., Scotto J., Degos F., Charnay P., Trepo C., Tiollais P. Detection of hepatitis B virus DNA in liver and serum: a direct appraisal of the chronic carrier state. Lancet. 1981 Oct 10;2(8250):765–768. doi: 10.1016/s0140-6736(81)90182-3. [DOI] [PubMed] [Google Scholar]
  4. Chu C. M., Liaw Y. F. Intrahepatic distribution of hepatitis B surface and core antigens in chronic hepatitis B virus infection. Hepatocyte with cytoplasmic/membranous hepatitis B core antigen as a possible target for immune hepatocytolysis. Gastroenterology. 1987 Jan;92(1):220–225. doi: 10.1016/0016-5085(87)90863-8. [DOI] [PubMed] [Google Scholar]
  5. Craxì A., Raimondo G., Longo G., Giannuoli G., De Pasquale R., Caltagirone M., Patti S., Squadrito G., Pagliaro L. Delta agent infection in acute hepatitis and chronic HBsAg carriers with and without liver disease. Gut. 1984 Nov;25(11):1288–1290. doi: 10.1136/gut.25.11.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hadziyannis S. J., Lieberman H. M., Karvountzis G. G., Shafritz D. A. Analysis of liver disease, nuclear HBcAg, viral replication, and hepatitis B virus DNA in liver and serum of HBeAg Vs. anti-HBe positive carriers of hepatitis B virus. Hepatology. 1983 Sep-Oct;3(5):656–662. doi: 10.1002/hep.1840030505. [DOI] [PubMed] [Google Scholar]
  7. Hadziyannis S. J., Sherman M., Lieberman H. M., Shafritz D. A. Liver disease activity and hepatitis B virus replication in chronic delta antigen-positive hepatitis B virus carriers. Hepatology. 1985 Jul-Aug;5(4):544–547. doi: 10.1002/hep.1840050404. [DOI] [PubMed] [Google Scholar]
  8. Lieberman H. M., LaBrecque D. R., Kew M. C., Hadziyannis S. J., Shafritz D. A. Detection of hepatitis B virus DNA directly in human serum by a simplified molecular hybridization test: comparison to HBeAg/anti-HBe status in HBsAg carriers. Hepatology. 1983 May-Jun;3(3):285–291. doi: 10.1002/hep.1840030302. [DOI] [PubMed] [Google Scholar]
  9. Negro F., Chiaberge E., Oliviero S., Hammer M., Berninger M., Canese M. G., Bonino F. Hepatitis B virus DNA (HBV-DNA) in anti-HBe positive sera. Liver. 1984 Jun;4(3):177–183. doi: 10.1111/j.1600-0676.1984.tb00925.x. [DOI] [PubMed] [Google Scholar]
  10. Paz M. O., Brenes F., Karayiannis P., Jowett T. P., Scheuer P. J., Thomas H. C. Chronic hepatitis B virus infection. Viral replication and patterns of inflammatory activity: serological, clinical and histological correlations. J Hepatol. 1986;3(3):371–377. doi: 10.1016/s0168-8278(86)80491-3. [DOI] [PubMed] [Google Scholar]
  11. Phillips M. J., Poucell S. Modern aspects of the morphology of viral hepatitis. Hum Pathol. 1981 Dec;12(12):1060–1084. doi: 10.1016/s0046-8177(81)80328-0. [DOI] [PubMed] [Google Scholar]
  12. Raimondo G., Burk R. D., Lieberman H. M., Muschel J., Hadziyannis S. J., Will H., Kew M. C., Dusheiko G. M., Shafritz D. A. Interrupted replication of hepatitis B virus in liver tissue of HBsAg carriers with hepatocellular carcinoma. Virology. 1988 Sep;166(1):103–112. doi: 10.1016/0042-6822(88)90151-1. [DOI] [PubMed] [Google Scholar]
  13. Ramalho F., Brunetto M. R., Rocca G., Piccari G. G., Batista A., Chiaberge E., Lavarini C., Dal Monte R. P., Carneiro de Moura M., Bonino F. Serum markers of hepatitis B virus replication, liver histology and intrahepatic expression of hepatitis B core antigen. J Hepatol. 1988 Aug;7(1):14–20. doi: 10.1016/s0168-8278(88)80502-6. [DOI] [PubMed] [Google Scholar]
  14. Rizzetto M., Verme G. Delta hepatitis--present status. J Hepatol. 1985;1(2):187–193. doi: 10.1016/s0168-8278(85)80766-2. [DOI] [PubMed] [Google Scholar]
  15. Rizzetto M., Verme G., Recchia S., Bonino F., Farci P., Aricò S., Calzia R., Picciotto A., Colombo M., Popper H. Chronic hepatitis in carriers of hepatitis B surface antigen, with intrahepatic expression of the delta antigen. An active and progressive disease unresponsive to immunosuppressive treatment. Ann Intern Med. 1983 Apr;98(4):437–441. doi: 10.7326/0003-4819-98-4-437. [DOI] [PubMed] [Google Scholar]
  16. Shafritz D. A., Shouval D., Sherman H. I., Hadziyannis S. J., Kew M. C. Integration of hepatitis B virus DNA into the genome of liver cells in chronic liver disease and hepatocellular carcinoma. Studies in percutaneous liver biopsies and post-mortem tissue specimens. N Engl J Med. 1981 Oct 29;305(18):1067–1073. doi: 10.1056/NEJM198110293051807. [DOI] [PubMed] [Google Scholar]
  17. Summers J. Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII. J Virol. 1975 Apr;15(4):946–953. doi: 10.1128/jvi.15.4.946-953.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yoo J. Y., Howard R., Waggoner J. G., Hoofnagle J. H. Peroxidase-anti-peroxidase detection of hepatitis B surface and core antigen in liver biopsy specimens from patients with chronic type B hepatitis. J Med Virol. 1987 Nov;23(3):273–281. doi: 10.1002/jmv.1890230310. [DOI] [PubMed] [Google Scholar]

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