Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1989 Jul;42(7):755–758. doi: 10.1136/jcp.42.7.755

Comparison of Y1 mouse adrenal cell and coagglutination assays for detection of Escherichia coli heat labile enterotoxin.

P A Chapman 1, C M Daly 1
PMCID: PMC1142029  PMID: 2668342

Abstract

A commercial coagglutination assay (COA; Phadebact LT-ETEC) was compared with a Y1 mouse adrenal cell assay for detecting the heat labile enterotoxin of Escherichia coli. Of four different media evaluated for use with the COA, only one (modified blood agar) gave a positive result with all strains known to produce heat labile enterotoxin. With modified blood agar, the COA detected 74 (85%) of 87 such strains. Eighty six strains negative by cell culture assay were also negative by COA, and one strain positive by COA could not be confirmed by cell culture. The Phadebact LT-ETEC kit provides a simple, sensitive, and economical method for detecting E coli heat labile enterotoxin.

Full text

PDF
755

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bramucci M. G., Holmes R. K. Radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae. J Clin Microbiol. 1978 Aug;8(2):252–255. doi: 10.1128/jcm.8.2.252-255.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brunton J., Hinde D., Langston C., Gross R., Rowe B., Gurwith M. Enterotoxigenic Escherichia coli in central Canada. J Clin Microbiol. 1980 Apr;11(4):343–348. doi: 10.1128/jcm.11.4.343-348.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bück E., Blomberg S., Wadström T. Enterotoxigenic Escherichia coli in Sweden. Infection. 1977;5(1):2–5. doi: 10.1007/BF01639100. [DOI] [PubMed] [Google Scholar]
  4. Carlson L. A., Ekelund L. G., Olsson A. G. Frequency of ischaemic exercise E.C.G. Changes in symptom-free men with various forms of primary hyperlipaemia. Lancet. 1975 Jul 5;2(7923):1–3. doi: 10.1016/s0140-6736(75)92948-7. [DOI] [PubMed] [Google Scholar]
  5. Chapman P. A., Mitchelmore D. L. A two-year survey of the incidence of heat-labile enterotoxin-producing Escherichia coli and other enteric pathogens in travellers returning to the Sheffield area. Epidemiol Infect. 1988 Oct;101(2):239–247. doi: 10.1017/s0950268800054157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chapman P. A., Swift D. L. A simplified method for detecting the heat-labile enterotoxin of Escherichia coli. J Med Microbiol. 1984 Dec;18(3):399–403. doi: 10.1099/00222615-18-3-399. [DOI] [PubMed] [Google Scholar]
  7. Finkelstein R. A., Yang Z. Rapid test for identification of heat-labile enterotoxin-producing Escherichia coli colonies. J Clin Microbiol. 1983 Jul;18(1):23–28. doi: 10.1128/jcm.18.1.23-28.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gilligan P. H., Robertson D. C. Nutritional requirements for synthesis of heat-labile enterotoxin by enterotoxigenic strains of Escherichia coli. Infect Immun. 1979 Jan;23(1):99–107. doi: 10.1128/iai.23.1.99-107.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gross R. J. Escherichia coli diarrhoea. J Infect. 1983 Nov;7(3):177–192. doi: 10.1016/s0163-4453(83)96953-0. [DOI] [PubMed] [Google Scholar]
  10. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Honda T., Akhtar Q., Glass R. I., Kibriya A. K. A simple assay to detect Escherichia coli producing heat labile enterotoxin: results of a field study of the Biken tests in Bangladesh. Lancet. 1981 Sep 19;2(8247):609–610. doi: 10.1016/s0140-6736(81)92745-8. [DOI] [PubMed] [Google Scholar]
  12. Levner M., Wiener F. P., Rubin B. A. Induction of Escherichia coli and Vibrio cholerae enterotoxins by an inhibitor of protein synthesis. Infect Immun. 1977 Jan;15(1):132–137. doi: 10.1128/iai.15.1.132-137.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rönnberg B., Wadström T. Rapid detection by a coagglutination test of heat-labile enterotoxin in cell lysates from blood agar-grown Escherichia coli. J Clin Microbiol. 1983 Jun;17(6):1021–1025. doi: 10.1128/jcm.17.6.1021-1025.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sack D. A., Huda S., Neogi P. K., Daniel R. R., Spira W. M. Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin. J Clin Microbiol. 1980 Jan;11(1):35–40. doi: 10.1128/jcm.11.1.35-40.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES