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PLOS ONE logoLink to PLOS ONE
. 2024 Sep 24;19(9):e0310918. doi: 10.1371/journal.pone.0310918

Demographic isolation and attitudes toward group work in student-selected lab groups

Mitra Asgari 1,*,#, Amy E Cardace 2,#, Mark A Sarvary 3
Editor: Gabriel Velez4
PMCID: PMC11421786  PMID: 39316619

Abstract

Small group work has been shown to improve students’ achievement, learning, engagement, and attitudes toward science. Previous studies that focused on different methods of group formation and their possible impacts mainly focused on measures of students’ academic ability, such as GPA, SAT scores, and previous familiarity with course content. However little attention has been given to other characteristics such as students’ social demographic identities in research about group formation and students’ experiences. Here, we studied the criteria students use to form lab groups, examined how the degree of demographic isolation varies between student-selected and randomly-formed groups, and tested whether demographic isolation is associated with group work attitudes. We used a pre-post survey research design to examine students’ responses in a large-enrollment biology laboratory course. Descriptive analyses showed that “students sitting next to me” (57%) followed by the combination of “students sitting next to me” and “friends” (22%) were the two most common criteria students reported that they considered when forming research groups. Notably, over 80 percent of students reported forming groups with those who sat nearby. We studied instances where students were isolated by being the only members of a historically marginalized population in their lab groups. The prevalence of demographic isolation in student-selected groups was found to be lower than in the simulated randomly assigned groups. We also used multilevel linear regression to examine whether being an isolated student was associated with attitudes about group work, yielding no consistent statistically significant effects. This study contributes to growing knowledge about the relationship between students’ demographic isolation in groups and group work attitudes.

Introduction

How the demographic composition of small groups influences students’ experiences has received increasing attention in recent years. Previous research has addressed group dynamics, students’ affective outcomes (i.e., attitude, feeling), or learning in college classrooms [e.g., 13]. Compared to competitive learning environments, working in collaborative learning settings could improve the opportunities for participation of historically marginalized groups in STEM, such as students who self-identify as female, African American, and Hispanic, among other identities [47]. However, poor group dynamics may lead to academic intimidation, where students may feel less competent [8], more anxious, quieter during group discussions [9], or underperform in the presence of their peers [10] due to phenomenon such as stereotype threat [11].

Cooperative learning, also known as small group work [12, 13], is philosophically rooted in social interdependence theory [14] and has become one of the most used and well-studied student-centered instructional practices [15]. In cooperative learning, students often work in small groups focusing on a set of shared learning goals and being assessed by the instructor both at the individual and the group level [16]. By incorporating small group work in classrooms, instructors can provide students with opportunities to discuss their ideas and perspectives with each other, provide and receive peer feedback, and develop and improve skills such as scientific reasoning, argumentation, communication, and teamwork [17, 18]. The value of group work for students’ learning and attitudes toward science has been studied and promoted for years [1921]. Meta-analyses of group work studies have shown improvement in students’ learning, interest in the subject, self-esteem, acceptance of diversity [22], academic achievement, persistence in coursework, and attitude toward learning [23]. More recent research has also shown enhancement of students’ overall achievement and learning [2426], engagement [27], use of high-order cognitive skills, attitudes toward science and persistence in STEM courses related to using group work [16, 26, 28, 29].

When permanent or long-term small group work is incorporated in classrooms, sometimes referred to as formal group work [16, 30], often one of the following group formation strategies is used. Groups can be formed by students, sometimes referred to as self-selected, student-selected, or student-formed groups, with little or no interference from an instructor. Alternatively, students can randomly be assigned to groups, where no criteria other than final group size are used [23]. Finally, groups could be created by using one or multiple criteria, often referred to as instructor-formed or instructor-assigned groups [31]. Based on existing literature in STEM education, the most common criteria instructors use when forming groups are measures of students’ academic ability, such as GPA, SAT scores, prior related courses, previous familiarity with course content [3236] or learning styles [3739]. However, little attention has been given to other students’ characteristics such as their social demographic identities and background when thinking about group formation.

Previous studies that have investigated group work quality, dynamics, and students’ learning in relationship to the demographic composition of the groups mainly focused on students’ gender identity. When working in small groups in STEM college classrooms, female students expressed less comfort than male students [8]. In an introductory psychology course, in groups of three, female students were less task-oriented in mixed-gender groups than in same-gender groups and they were less talkative when they were solo in groups compared to male students [10]. In another study, female engineering students showed more anxiety in female-minority groups in their first year of college. Findings of the same study indicated less verbal participation of female students in female-minority groups regardless of their academic year in college compared to the sex-parity groups and female majority groups [40]. Student collaboration, observed as equitable group work processes, was stronger in gender-balanced groups compared to all-male or solo-male groups [41], although no difference was observed in students’ performance [10, 41]. In another study, researchers found that grouping by gender mostly impacts students’ attitudes toward instruction rather than their performance and that gender-balanced and female-only groups are the most effective [42].

These findings suggest the possibility of similar experiences by other social minority groups when it comes to group work. A study of small groups in STEM college classrooms showed that students identified as Underrepresented Minority (URM) students reported higher levels of social-comparison concern than the majority students [8]. During peer discussions in a large introductory biology course, underserved American and Asian-American students showed a stronger preference for the role of listener over leader/explainer when compared to white Americans [9]. In an introductory sociology course, when the associations between leadership, sex, race, and course performance were investigated in teams created by the instructor, researchers found that white students had more leadership roles in teams, received higher grades, and were evaluated higher than students of color [43]. The research findings related to students from other social minority groups such as first-generation college students or international students are even more scarce. During peer discussions, international students also showed a stronger preference for having the role of listener over the leader/explainer role when compared to white Americans, and they reported experiencing higher anxiety during peer discussions [9].

In addition to the above findings, a body of research shows that when given the opportunity, students in science classrooms tend to create more homogenous groups based on gender and ethnicity [29] and based on a combination of gender identity, academic, and personality characteristics [44]. We were eager to explore similar questions in a smaller learning setting, the laboratory part of a large-enrollment science course. This study was conducted to understand the students’ considerations when forming groups, the frequency of demographic isolation in student-formed groups, and the relationships between group compositions and students’ attitudes about group work. We used a pre-post survey approach and included questions to learn about students’ demographic identities, criteria considered when forming their groups, and group work attitudes. We assessed the frequency of students’ demographic isolation in self-selected groups compared to hypothetical randomly-formed groups. We use the term demographic isolation to describe the situation where a student is the only member from a particular demographic group in their lab group (e.g., the only female or ethnic minority student in a group). We also investigated whether this isolation is associated with differences in students’ attitudinal group work scores. Our research questions are listed below:

  • Q1. What criteria do students report that they consider when forming lab groups?

  • Q2. How does the composition of student-selected groups differ in terms of demographic isolation when compared to hypothetical randomly-formed groups?

  • Q3. Do students’ attitudes toward group work vary between students who were demographically isolated in groups and those who were not?

Materials and methods

Study context

Course setting/Information

We conducted our study in an introductory biology course at a large research university in the northeastern United States. This two-credit-hour inquiry-based laboratory course is required across many biology-focused majors. Approximately 400 first- and second-year students enroll in the course each semester. The course consists of a weekly 50-minute lecture [45, 46] and a 3-hour laboratory (hereafter lab) session [47], both using evidence-based teaching practices. In this course, the students could enroll in any of the twelve lab sections offered each semester. Group formation and group work take place in the lab sessions, which were held in multiple small rooms with traditional fixed tables and movable seating, each hosting a maximum of 18 students. Labs were led by graduate teaching assistants (hereafter GTA) and offered guided inquiry learning environment throughout the semester [48, 49]. In both lecture and lab environments, students were free to self-select both their seats and their lab groups.

Group formation and group work in the lab

For the first three weeks of the semester, students worked together informally during labs on activities that were graded and assessed at an individual level. During the fourth week in the lab, GTAs asked students to form groups of three (hereafter student-selected groups). Students usually stayed in these groups for the rest of the semester. In cases where the number of students per lab was not perfectly divisible by three, a few groups of 2 were allowed to be formed. For the rest of the semester, students were expected to work with their group members on various activities such as designing and conducting an experiment, collecting and analyzing data, and group presentations. After group formation in week 4, students were assessed and graded both at the individual and group levels for the rest of the semester.

Study design and data collection

We used a survey research approach to quantitatively address three questions about (1) the criteria students considered when forming their lab groups, (2) the prevalence of students being demographically isolated in their self-selected groups compared to randomly-formed groups, and (3) relationships between being demographically isolated and attitudinal outcome measures. The course and survey administration timelines are summarized in Fig 1, specifying when groups were formed and the related data were collected. To account for possible variation between semesters, we studied these questions for two consecutive semesters, Spring and Fall 2019. The pre-and post-surveys were conducted online via Qualtrics (Seattle, WA). In the pre-survey, participants responded to a combination of questions asking about their demographics, academic backgrounds, and attitudes toward group work. In the post-survey, in addition to the same pre-survey questions, students were asked to share the criteria they used to form a formal lab group in week 4.

Fig 1. The timeline and processes of group formation and data collection.

Fig 1

Ethics statement

This project was approved by the Cornell University Institutional Review Board and has been granted an exemption from IRB review (#1901008516).

Participant recruitment

Participants were students who enrolled in the course. To recruit participants, we used the course learning management system (LMS) at the beginning and end of each semester to share a message providing the project summary information, with a link to the survey and a written consent form with students. A few bonus course credits for responding to each of the pre-and post-surveys were also provided to students. Responses from students who did not provide consent or were minors were removed from the study before the analyses.

Survey measures

Students’ demographic information

The demographics we examined in this study were students’ gender and ethnic-racial identities, college generation status, and international student status. We focused on the first two demographics due to the vast evidence about the historical marginalization of individuals who identify as female, Black/African American, Hispanic/Latinx, and Native American/American Indian/Indigenous American in STEM higher education [50]. Previous research also shows that first-generation college students, individuals whose parents do not have a 4-year bachelor’s degree, face various academic challenges in STEM courses [5153]. We also focused on international students because they are often in the minority in US undergraduate college classrooms, and there is little published research about this population in the groupwork STEM education literature [see 9]. In this context, international students are referred to citizens of other countries who came to the United States for post-secondary education [54]. These data helped us assess the demographic composition of student-selected groups and were used as independent variables in multi-level regression analyses predicting groupwork attitudes.

Criteria students considered when forming lab groups

In addition to demographic information, a multiple-choice question was included in the post-survey each semester to learn about the criteria students considered when forming their group during the fourth week of the lab. Students could select more than one option from a list of twelve responses including the following: “friends”; “students sitting next to me in the lab”; “students’ GPA and familiarity with the biological concepts”; “students’ gender identity”; “students’ race/ethnicity”; “students’ year in college.” This survey question with full list of response choices provided to students, can be found in the supporting information document. In terms of the logic behind the answer choices selected, when the survey questions were being developed the authors considered the most probable choices such as “friends”, “proximity in the lab” and explored the STEM education literature for the most common visible and hidden identities and characteristics students may identify with and commonly studied. We thought of identities that could be visible from the first day of the class when students meet each other like “racial/ethnic identity” and the ones that may be less evident or hidden but students could possibly share with each other in the first 3 weeks of the lab and before forming groups, such as “being an international student or not”. In the post-survey before students see this multiple-answer question, they were also asked to respond to an open-ended question of “Which criteria did you use to form your research group?”. The preliminary coding of students’ responses for the open-ended version of this question yielded similar findings and no additional themes. Thus, to avoid redundancy, here we only present outcome of the multiple-choice question.

Student attitudes about group work

We adapted and re-validated an item set from the Student Attitudes towards Group Environments (SAGE) survey [55] to assess students’ group work attitudes in this context. The original questionnaire had 54 five-point Likert survey items (strongly agree, agree, undecided, disagree, strongly disagree) categorized by four factors: quality of product and process, peer support, student interdependence, and frustration with group members.

Our process of evaluating the original SAGE items and selecting a subset for current use leveraged strategies for improving validity during instrument design [56]. We mapped the factors of interest to particular items and engaged content and course experts (M.A and A. E. C) to check the items for student accessibility. Considering previously reported relationships between demographic group composition and student group processes or performance noted above [10, 41, 43], we chose to focus on two particular factors, work quality and interdependence. Researchers affiliated with the course screened the items to make sure they were logically related to the students’ lab group activities (M.A and M. A. S).

This process yielded fourteen items that were most relevant to our study (see S1 Table). We analyzed data from all respondents who replied to all 14 items, using a generalized Rasch model for polytomous data [57]. We found that the14-item instrument showed strong internal consistency statistics of 0.79 for the pre-test (n = 547) and 0.84 for the post-test (n = 281). In addition to establishing content validity as noted above, we also checked estimates from the model for expected patterns; all weighted mean square fit statistics were within the expected range (0.77–1.33) and the response category thresholds within each item were ordered from low to high as theorized [57].

Participant demographics

The data set included 1148 responses, including pre-and post-surveys for both semesters. The response rate of students after data cleaning was 74% and 80% for the spring and fall semesters, respectively. The participation rates and the demographic breakdowns of participants were generally consistent between the two semesters (Table 1). In both semesters, females outnumbered males by almost two to one. The responses of students who selected non-binary or prefer not to say options had to be eliminated from the quantitative analysis due to their small sample size (0.7% and 0.6% in Spring and Fall 2019). White and Asian students comprised the majority of students in both semesters. Due to the relatively small number of students who self-identified with historically marginalized races and ethnicities, we pooled their responses as a single category for the quantitative analyses. This group included students identifying as (1) Black/African American, (2) Hispanic/Latinx, (3) Native American/American Indian, (4) Native Hawaiian or Other Pacific Islander, (5) Middle Eastern or North African, or (6) Some Other Race, Ethnicity, or Origin. We represent this combined group with an acronym to identify the primary groups, BHN+. As discussed by previous studies [cf. 9, 5860], student populations from these backgrounds share historical marginalization and present underrepresentation in American post-secondary STEM environments [61]. Although we acknowledge these subgroups may have varying experiences, exploring those differences is outside the scope of this study. The first-generation college students and international students constituted less than 20% and less than 10% of the participants, respectively (Table 1).

Table 1. Response rate and demographic overview of students who participated in the study over the two semesters.

Spring 2019 Fall 2019
Number of students enrolled in the course 378 392
Number of respondents* 281 315
Response rate 74% 80%
Gender
    Female 64% 65%
    Male 35% 34%
    Nonbinary 0.7% 0.6%
    Prefer not to say 0.3% 0.3%
Race/Ethnicity
    White 37% 40%
    Asian 32% 34%
    Hispanic, Latinx, or Spanish origin 13% 9%
    Black or African American 11% 11%
    American Indian or Alaska Native 0% 0%
    Native Hawaiian or Other Pacific Islander 0% 1%
    Middle Eastern or North African 2% 1%
    Other Race, Ethnicity, or Origin 5% 3%
First-generation college status 17% 16%
International status 4% 7%

Statistical analyses

We conducted three types of data analyses to address the research questions. All data used are shared as supporting information (S2S4 Files). First, descriptive analyses were used to explore the criteria that students reported they considered when forming their lab groups and to analyze the demographic composition of student-selected groups of three. During this descriptive phase, we checked for the frequency of isolation of female students, BHN+ students, first-generation students, and international students. Second, to examine whether student-selected groups yielded the same degree of student isolation as randomly assigned groups, we simulated random groupings by randomly assigning students to hypothetical groups of three within their lab sections with 100 iterations, yielding 100 possible versions of the random group assignments. We calculated the percentage of isolated students in each iteration and created a mean percentage for all 100 iterations for each demographic variable (female, BHN+, first-generation, and international students). We also compared the sample of 100 simulated percentages of isolated students to the actual percentage from student-selected groups and tested for a statistically significant difference using two-tailed one-sample t-tests.

Finally, we used three-level linear regression models (sometimes referred to as HLM or MLM) to examine whether students’ demographic isolation status influenced their group work attitude scores. Given the clustered nature of our data set, we used random variables to more accurately account for variation due to the students’ assigned GTAs, and lab group [62, 63]. In addition to student demographics, we included binary variables showing whether students were isolated in their lab group by gender, BHN+, first-generation, or international status.

Given that this study required both the estimation of student scores and the use of those scores as the dependent variable in linear regression, we used item response theory (hereafter IRT) and specifically a partial-credit model to estimate item difficulties and student scores [57]. We analyzed two types of student scores, Expected A Posteriori (EAP) scores that average multiple estimates, and plausible values (PV) that include a wider range of possible student scores to account for error more accurately. More details about these statistics can be found in the supporting information document (S1 File).

Given the continuous nature of the EAP and PV scores, we used a three-level linear regression with the xtmixed command in Stata15 [64], where each student (i) has a particular group (j) under a particular GTA (k):

Attitudes-post scoreijk = β0 + β1(Attitudes-pre scoreijk) + β2(Isolated-Femaleijk) + β3(Isolated-First-generationijk) + β4(Isolated-Internationalijk) + β5(Isolated-BHN+ijk) + β6(Femaleijk) + β7(First-generationijk) + β8(Internationalijk) + β9(BHN+ijk) + B10(Semesterijk) + u1(GTA)jk + u2(group)jk + εijk

  • ○ Attitudes-post score is group work attitudes post plausible value score

  • ○ Attitudes-pre score is group work attitudes overall pre plausible value score

  • ○ Isolated-Female, Isolated-first-generation, Isolated-International, and Isolated-BHN+, are four binary variables reflecting if a student with these demographics were isolated in their lab group (0 = not isolated, 1 = isolated)

  • ○ Female, First-generation, International, and BHN+ are four binary variables describing whether a student was from any of these demographics (0 = no, 1 = yes)

  • ○ Semester is the semester a student took the course (1 = spring, 2 = fall)

  • ○ GTA identifies the GTA-led lab section, and the group identifies the lab group

NOTE: The estimated random coefficients for GTA and lab groups are represented by u1 and u2, respectively. The estimated error is represented by ε.

Results

Question 1: What criteria do students report that they consider when forming lab groups?

In response to the post-course survey question about the criteria considered when forming their lab groups, a majority of students reported forming groups with peers sitting next to them in the lab (59% in Spring 2019 when n = 236; 54% in Fall 2019 when n = 208). The next common criteria selected were peers sitting next them plus friends (27% in Spring2019 and 16% in Fall 2019), followed by people sitting around them, friends, and other criteria (11% in Spring 2019 and 25% in Fall 2019) and only friends (3% in Spring19 and 5% in Fall 2019) (Fig 2). In the Fall 2019 post-course survey a few additional questions were asked to better understand the stability of lab groups and seating choices. Most students (93%) reported that they stayed in their original lab groups for the rest of the semester and did not change their groups. Students also stated that on the first day of class, they chose the first available lab seat (74%) or sat by their friends (21%) in response to the questions on how they decided where to sit in the lab. Most students (87%) also reported that they did not change where they sat in the lab between the first week of class and when forming their groups in week four.

Fig 2. The criteria most students reported considering when forming groups.

Fig 2

The “sitting next to me (only)” or “Friends (only)” represents the students who only selected one of these two items as criteria to form groups. Students’ selection of more than one criterion is shown with a + sign.

Questions 2. How does the composition of student-selected groups differ in terms of demographic isolation when compared to hypothetical randomly-formed groups?

If students’ processes for selecting groups were not influenced by demographics, then we would expect to see similar demographic compositions for both self-selected and randomized groupings. To examine these compositions, we first analyzed the responses of students for whom data was available for all students in their group. This subsample included 303 students in 101 groups of 3 over the two semesters. The analysis of these data showed that 18% of these groups had isolated female students, 29% of groups had isolated BHN+ students, 31% of groups had isolated first-generation students, and 12% of groups had isolated international students. In terms of the number of students rather than groups, 6% of female students, 10% BHN+ students, 10% of first-generation students, and 4% of international students were isolated in their lab groups.

Among the 303 students included in this analysis, the distribution of demographic categories generally mirrors that of the larger sample: female students comprised 62%; BHN+ students comprised 28%, first-generation students comprised 12%, and international students comprised 6%. Given that these demographic groups were not equally prevalent in the course, the social ramifications of being isolated would vary depending on the particular demographic. We examined this variation by calculating the percentage of students in each demographic group who were isolated. These adjusted percentages show a relative increase in the prevalence of isolation yielding 9% for female students, 33% for BHN+ students, 79% for first-generation college students, and 71% for international students.

Comparing the percentages of isolated students in hypothetical randomly assigned groups to the actual percentages presented in Table 2, we found more isolation in the randomly assigned groups among female and BHN+ students. The percentage of groups with an isolated female student rose from 18% in actual self-selected groups to 24% in simulated random groups. The percentage of groups with an isolated BHN+ student rose from 29% in the actual self-selected groups to 38% in the simulated random groups. In both cases, one-sample t-tests show statistically significant differences (females: t = 25.07, d.f. = 99, p<0.001; BHN+: t = 27.45, d.f. = 99, p<0.001). However, the percent of groups with isolated first-generation and international students did not show the same degree of variation between actual student-selected groups and simulated random groups (see Table 3). Examining the data for international students, there was a very small difference between the simulated mean percentage (12.8%) and the actual mean percentage (12.0%). This small difference would only generally affect one student in a class of one hundred students, yet it was statistically significant (t = 7.50, d.f. = 99, p<0.001). There was no statistically significant difference between the actual and simulated percentages for first-generation students (t = -1.71, d.f. = 99, p = 0.09).

Table 2. Actual prevalence of demographic isolation in student-selected groups.

Students’ Demographics Actual percent of all groups with an isolated student Actual percent of all students who experienced isolation Actual percent of students in each demographic group who experienced isolation in their group
Female 18% 6% 9% of female students
BHN+ 29% 10% 33% of BHN+ students
First-generation 31% 10% 79% of first-generation students
International 12% 4% 71% of international students

Table 3. Percent of students and groups with demographic isolation in simulated randomly assigned groups.

The values are grand-means for each demographic variable which are the average percentages of isolated students over 100 simulations.

Students’ Demographics Simulated percent of groups with an isolated student* Simulated percent of all students who were isolated* Simulated percent of students in each demographic group who experienced isolation in their group
Female 24% 8% 13% of female students
BHN+ 38% 14% 44% of BHN+ students
First-generation 31% 10% 82% of first-generation students
International 13% 5% 76% of international students

*Based on 100 randomly assigned groupings.

At the individual student level, these differences between self-selected and hypothetical random groups yielded measurable differences in the percentages of female and BHN+ students who were demographically isolated shown in Fig 3. These differences were even higher when adjusting for the proportion of students from each demographic group in the course. (See the last columns in Tables 2 and 3).

Fig 3. Percent of students demographically isolated in actual self-selected versus simulated randomly assigned groups.

Fig 3

Question 3: Do students’ attitudes toward group work vary between students who were demographically isolated in groups and those who were not?

We used two multi-level regression models to test for statistically significant relationships between demographic isolation and students’ group work attitude scores (see S2 Table). Model 1 regressed the pretest attitudinal scores on the demographic and isolation variables and random variables that identified each student’s teaching assistant and lab group. This model addressed whether students entered the course with group work attitudes that varied by isolation status. Model 2 regressed the posttest attitudinal scores on the pretest estimates and the other independent variables used in the first model. This model addressed the degree to which student scores showed pre-post change, and whether students exited the course with group work attitudes that varied by isolation status after controlling for their pretest scores.

Overall, the regression analyses showed no consistent negative associations between demographic isolation and students’ groupwork attitudes, both as they began coursework and at the end of the course (Table 4). For the first model that used the pretest group work score outcome variable, the only statistically significant effect was a positive effect for students who were isolated as BHN+ students in their lab groups (see S2 Table). This effect was not consistent across the regressions we ran on plausible values, which better account for measurement error (see S3 Table). In the models we ran to examine associations with the posttest group work scores, there was no statistically significant effect of any demographic isolation variable. While the female and international demographic variables did show statistical significance using EAP scores, those effects were not consistent across the more accurate plausible value trials. The only independent variable that showed a consistent and statistically significant relationship with the group work post-test score was the pretest score (coeff. = 0.69; p<0.001).

Table 4. Results from multi-level linear regression on posttest attitudinal scores controlling for pretest scores and estimating demographic and isolation variable coefficients (n = 185).

coeff. s.e. p-value
Fixed Effects Pre-Groupwork EAP 0.689 0.072 0.000 ***
Female -0.301 0.136 0.028 *
BHN+ 0.115 0.180 0.523
FGC 0.290 0.520 0.577
International 1.004 0.463 0.030 *
Isolated Female -0.057 0.257 0.824
Isolated BHN+ -0.023 0.256 0.927
Isolated First-generation -0.155 0.543 0.775
Isolated International -0.494 0.584 0.397
Semester -0.090 0.136 0.509
Random Effects GTA 0.000 -
Group 0.286 0.131

* Denotes statistical significance at the 0.05 level, ** at the 0.01 level, and *** at the 0.001 level. LR test vs. linear model did not show a statistically significant difference: Prob > chi2 = 0.5243

Discussion

Group work is a commonly used student-centered pedagogical approach in college classrooms. Given concerns about the social-emotional challenges some students face when isolated by particular identities, educators can benefit from a better understanding of student behaviors and related social experiences in these group work settings. In this examination of student survey data, we found that the majority of students reported casually forming groups with peers who were sitting around them and that their process yielded groups with less demographic isolation than would have resulted from randomly-formed groupings. Further, while demographic isolation has been identified in previous studies as presenting a variety of challenges for students, our quantitative analyses did not show that demographic isolation was associated with variation in students’ group work attitudes.

Students mainly reported choosing groups based on peers sitting close to them in the lab, selected as the sole factor or in addition to others such as friends. We acknowledge that current study cannot tease apart the conscious and unconscious biases that students’ likely hold when locating themselves in group settings, interacting with new people, and navigating student-selected group formations. We also note that students may not be entirely forthcoming about their considerations. Yet, there was a consistent trend in students’ responses that proximity and friendship were relevant for their choice of lab partners. Further, very few students reported that they intentionally considered demographics in their group choice. In the second semester post-survey, to better understand the consideration behind seating choices, when we asked students where in the lab, they decided to sit on the first day of class, most students (74%) mentioned choosing the first available seat they found or sat by their friends (21%); majority of them also reported not changing their seat between week 1 and when forming their lab groups in week 4. However, where students reported to sit could be related to the conscious and unconscious biases that students’ likely hold which were beyond the scope of this study. Furthermore, we did not investigate the relationship between criteria students reported considering when forming groups and their attitude about group work. However, previous research that focused on informal group composition and group work, found that having a friend in a group was the main predictor of students’ comfort in groups, a tendency reflected in our findings as well [65].

In our study, student-selected groups yielded another benefit for students, less demographic isolation when compared to hypothetical randomly formed groups. A previous study looking at the composition of informal groups in a large-enrollment biology classroom also showed students mostly formed homogenous groups in terms of ethnicity and gender [29]. This phenomenon is known as “homophily” [66] and is defined as the inclination of individuals to move toward and work with others who are like themselves. Our findings show this phenomenon even in lab settings where students are only forming groups from a small pool of peers (n ~ 15–18). Further, the prevalence of an identity group in the course related to the degree of isolation. Because the proportion of students who identified as first-generation or international was low in the course, there was more likelihood for those students to be isolated in lab groups, whether they self-selected or were randomly assigned. In contrast, females were consistently over-represented in this course, which led to fewer possibilities of being the only female in a lab group. The degree of isolation will always be dependent on the demographic composition of a particular context. Thus, we do not know if the statistical significance of gender and racial-ethnic demographics in this case will also be evident in other settings where the demographic composition of the cohort is meaningfully different. There must be a large enough number of students in a demographic category so that they have opportunities to change the composition of their group, and enough instances of that change to show measurable effects in a quantitative analysis.

Our findings indicate that allowing students to choose their groups can serve a self-protective purpose because they can avoid demographic isolation. While this study does not explain the mechanism by which self-selected groupings yield less isolation, the fact that there were statistically significant reductions in isolation when students self-selected warrants further inquiry. This is extremely important when we aim to support historically marginalized groups in STEM. Further, we note the difference between demographic traits that are visually evident to other students and those traits that are hidden. Henning et al. (2019) [67] noted the particular challenges that small-group coursework presents for students with particular political, religious, gender, and sexual identities. Given the hidden nature of some of these identities, instructors are typically unable to systematically avoid isolating students by assigning groups based on identities that are visually evident or commonly tracked. Student-selected groups could allow students to choose peers they perceive to be allies in their groups even in the case of hidden identities by considering only those identities that seem important to them. This can be facilitated by providing time for students to get to know each other and form trust before forming groups, such as the first 3 weeks of informal group interaction in this study. In these conditions students can choose groups to avoid the types of demographic isolation that they perceive as negative. For that reason, we estimate that the impact of any measurable demographic isolation that remains after students form groups may be minimized.

Other studies show the benefit of student-selected groups compared to randomly-formed groups when it comes to team experience [68]; student satisfaction and grades [69]; and conflict resolution, communications, enthusiasm, and overall group work attitude [70]. Students who self-select their groups spent the most amount of time outside the classroom working on course materials with their group members and felt more connected to their group members than the students in instructor-designed groups or randomly-formed groups [44]. A more recent study that assessed the impact of different group formation types on students’ attitudes towards group work in large biology classrooms, found that heterogeneous groups in terms of competence have higher group work attitudes and groups formed by students were as heterogeneous as groups formed by the instructor [2].

One argument against student-selected groups is that they are often less diverse, either demographically or academically. It has been argued that more homogenous groups could negatively influence students’ performance or attitudes. While students may often form more demographically homogenous groups ([29, 44], current study), these groups do not necessarily show lower outcomes [2, 44, 6970]. We also considered the argument that people do not get to choose with whom they work in professional workspaces, and by allowing students to self-select groupmates, we are preventing our students from learning how to work with people from diverse backgrounds. While this is a valid concern and educators should help students develop skills to work with people from diverse backgrounds, we reason that making students work in particular groups without considering their preferences also does not reflect most workplace conditions where groups typically work together over longer periods of time and employees have some degree of self-selection.

These arguments may not adequately account for the potential harm of group formation methods that ignore student preferences, including the tendency for assigned groups to have negative impacts related to satisfaction, trust, and divisiveness [71]. this study suggests intentionally offering opportunities for students to build trust with others before allowing them to select their own groups. Building on this idea, we suggest that instructors’ multiple goals may require multiple distinct actions. Specifically, some goals, such as promoting student satisfaction and avoiding demographic isolation can be targeted by allowing student-selected groups. Other goals, such as improving students’ cultural competency skills, may require other instructional support. In practice, teaching students to work well with diverse groups of people likely requires actions beyond assigning the particular group compositions that instructors have adequate data to prescribe. While cultural competency courses can influence student attitudes in positive ways [72], we suggest there are also simple strategies instructors can use to allow student-selected groups while also providing opportunities for students to practice cultural competency skills by creating course activities that require inter-group collaboration.

The multilevel regression findings suggested that students’ attitudes towards group work did not vary with being demographically isolated in lab groups. Yet, given prior research about the interpersonal challenges that isolated students sometimes face, we acknowledge that this lack of effect may not hold for all students in all contexts. Regression findings are inherently generalizations, so they are not useful for explaining the unique experiences of individual students. Further, this study took place in a particular setting where students may have been less likely to struggle with being demographically isolated for several reasons: this sample of students was from a highly competitive institution where they were overwhelmingly high-achieving students with strong academic identities which likely minimizes both the range of academic behaviors within groups and the range of group work attitudes; student-selected groups allowed students to avoid being isolated in ways that would have felt challenging for them; and there was pedagogical support explicitly about effective group work throughout the course. Regarding the attitudinal measure, there was only a small degree of pre-post change during a course with a great deal of group work, which suggests this particular outcome measure may not be very prone to change. It is also crucial to note that these findings do not dispel concerns about other ways demographic isolation negatively influences students. Relationships between demographic isolation and other outcome measures in other contexts need to be explored as well.

Conclusions

This study provides unique evidence about how students form groups and how their choices reduce the chances of being demographically isolated in groups. In this work, we focused on comparing the composition of student-selected groups with hypothetical randomly-formed groups. Our study shows that when given the opportunity, students mostly form groups with peers sitting near them in the classroom while a much lower proportion of students reported only considering friendship as a criterion to form groups. Groups that were formed by students led to less isolation of students identified as female and BHN+ compared to randomly-formed groups. While demographic isolation has been identified in previous studies as presenting a variety of challenges for students, our quantitative analyses did not show that demographic isolation was associated with variation in students’ group work attitudes. Although instructors can implement group formation systems to create groups with particular characteristics, students may benefit more from self-selected working groups based on identities that seem important to them with other instructional supports for practicing collaboration with diverse peers. If the student-selected group formation approach is used, as educators, we recommend doing frequent check-ins to learn about group dynamics during the semester and designing activities that encourage within and between-group interactions to enhance the engagement of students with diverse identities. Comparing our findings with other group formation methods in other settings and teasing apart the conscious and unconscious biases that students’ likely hold in group selection and interactions go beyond the scope of this current study and would be a valuable next step to be explored.

Supporting information

S1 Table. Groupwork attitude items.

(DOCX)

pone.0310918.s001.docx (14.7KB, docx)
S2 Table. Multi-level linear regression output for group work EAP pretest and posttest scores.

(DOCX)

pone.0310918.s002.docx (17.6KB, docx)
S3 Table. Multi-level linear regression output for group work plausible values across five trials to check for accuracy.

(DOCX)

pone.0310918.s003.docx (24.2KB, docx)
S1 File

(PDF)

pone.0310918.s004.pdf (118.7KB, pdf)
S2 File. Data used in group selection criteria.

(XLSX)

pone.0310918.s005.xlsx (28.3KB, xlsx)
S3 File. Data used in randomized grouping analyses.

(XLSX)

pone.0310918.s006.xlsx (33.7KB, xlsx)
S4 File. Data used in MLM analyses.

(CSV)

pone.0310918.s007.csv (49.4KB, csv)

Acknowledgments

We would like to thank the students who agreed to participate in this study, especially for their time completing the survey and sharing their experiences. We also appreciate support from the Active Learning Initiative at Cornell University.

Data Availability

This study involves human research participant data. All relevant data that were used in analyses are first de-identified and then provided as Supporting Information files to allow to replication of the results of the study.

Funding Statement

The author(s) received no specific funding for this work.

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Additional Editor Comments:

In general, this is a strong paper that I believe will make a contribution to the literature and is relevant for this journal. My main concerns are very much in line with those of the reviewers, and I believe revolve around considering some important qualifiers and possible limitations. In particular, the reviewers raise a number of points around possible alternative interpretations of findings (such as hidden identities, the group formation and influence, and unconscious bias).

I think the deeper points involve self-reporting. While I recognize it is often necessary, I think it is important to qualify and reshapes what can be asked from data. I very much believe these concerns can be addressed, but also consider them to be consequential enough to merit a major revision.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: No

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: This study investigates how demographic isolation in student-selected lab groups affects students' attitudes toward group work. Using a pre-post survey design, the authors collected data from a large-enrollment biology laboratory course to examine the criteria students use to form lab groups, the prevalence of demographic isolation in student-selected versus randomly-formed groups, and the association between demographic isolation and students' group work attitudes. The study found that students primarily formed groups with peers sitting near them, resulting in less demographic isolation than randomly-formed groups. The regression analyses showed no consistent negative associations between demographic isolation and students' attitudes toward group work.

This study is relevant given that group work is a commonly used student-centred pedagogical approach in college classrooms. Given concerns about the social-emotional challenges some students face when isolated by particular identities, educators can benefit from a better understanding of student behaviours and related social experiences in these group work settings.

However, I have some comments and questions that could potentially improve the overall quality of the study:

- Clarification on survey options: In the post-course survey, students were given six choices for criteria they considered when forming their lab groups: “friends,” “students sitting next to me in the lab,” “students’ GPA and familiarity with the biological concepts,” “students’ gender identity,” “students’ race/ethnicity,” and “students’ year in college.” Could you please clarify how you determined these specific options for the survey? Were they based on previous literature, preliminary research, or another rationale?

- Potential bias in group formation criteria: Regarding the criteria provided for group formation, don't you think limiting the possible choices to a relatively small number may bias the study's results? Additionally, do you consider self-reporting problematic, given the bias that political correctness may introduce? How did you address these potential biases in your research?

- Sample size clarification: When you mention, "The responses of students who selected non-binary or prefer not to say options had to be eliminated from the quantitative analysis due to their small sample size," how small was this sample size? While the answer is provided in Table 1, adding this information directly to the text would make the paragraph more straightforward. Could you include the specific numbers in the text for clarity?

- Unconscious bias in seating choices: The authors state, "In this examination of student survey data, we found that the majority of students reported casually forming groups with peers who were sitting around them and that their process yielded groups with less demographic isolation than would have resulted from randomly-formed groupings." Could it be that students choose their seats based on unconscious bias towards minorities? How did your analysis account for potential unconscious biases in seating choices?

Reviewer #2: Accept with minor revisions

This study aimed to address the following 3 questions:

Q1. What criteria do students consider when forming lab groups? Q2. How does the composition of student-selected groups differ in terms of demographic isolation when compared to randomly-formed groups? Q3. Do students’ attitudes toward group work vary between students who were demographically isolated in groups and those who were not?

Questions 2 and 3 were adequately address in the data, results section, and discussion. The finding that student-selected groups decreased demographic isolation is novel and provides instructors with both important practical information information to consider when assigning group work. Especially, as the paper stated, group work is one of the most common student centered practices in the college classroom. This finding, coupled with the positive perception of group work from the students provides instructors with more information to consider when attempting to create an comfortable and inclusive classroom and researchers foundational data to build upon when looking at student centered practices that are predicated on formal group work.

While question 1 was addressed by the research team, there are still unaddressed questions. The fact that students working in informal groups for 3 week prior to creating teams was not addressed as a potential impact on team selection. Figure 2 shows that most students opted to work with students near them, but the fact that those students had potentially worked together and formed trust over the course of those 3 weeks is not addressed. In a follow up study, it would be interesting to look at the group of students that did not team up with those around them, presumably those students that they’ve been working with, and collect qualitative data on their reasoning for picking a team with students they did not work with during the initial 3 weeks of the lab. This could potentially help address the claim on page 21 “students can choose groups to avoid the types of demographic isolation that they think will likely cause them harm.”. While that data does show that allowing students to choose their groups can allow them to actively avoid demographic isolation, which is interpreted as a self protective mechanism, and is important in supporting historically marginalized groups in STEM, the data does not support that there is the same impact for hidden identities (claim made on page 20). The two potentially hidden identities surveyed for are first generation status and international status. These two identities may not be immediately visible from someones physical appearance and showed no statistically significant difference in demographic isolation percentage between the classroom and simulated data. More information is needed for the authors to properly make this claim.

The suggestions for instructors in the discussion section felt thorough and nuanced. They addressed the different arguments both for and against allowing students to choose their own groups. Specifically, the argument around cultural competency was salient. The following below was a great addition to the ongoing conversation on this topic and gave practical considerations for instructors when it comes to harm mitigation and the extra emotional labor that marginalized students take on in group interactions.

"Other goals, such as improving students’ cultural competency skills, may require other instructional support. In practice, teaching students to work well with diverse groups of people likely requires actions beyond assigning the particular group compositions that instructors have adequate data to prescribe. While cultural competency courses can influence student attitudes in positive ways (Patterson et al., 2018), we suggest there are also simple strategies instructors can use to allow student-selected groups while also providing opportunities for students to practice cultural competency skills by creating course activities that require inter-group collaboration.

Overall, this is a well-done paper and resulted in an interesting observation on the topic of demographic isolation in group work.

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Reviewer #1: No

Reviewer #2: No

**********

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PLoS One. 2024 Sep 24;19(9):e0310918. doi: 10.1371/journal.pone.0310918.r002

Author response to Decision Letter 0


24 Aug 2024

Rebuttal letter

Re: manuscript PONE-D-24-13332

August 15, 2024

To: The Academic Editor PLOS ONE

Dear Dr. Velez,

Firstly, the authors would like to express their gratitude for the constructive feedback and suggestions received from PLOS ONE reviewers and the academic editor. We are delighted that PLOS ONE appreciates the value of our study and is interested in publishing this work upon addressing the received comments and suggestions. Below, we have responded to and addressed the main suggestions and concerns raised by the academic editor and reviewers. The related changes have been made and marked within the text as instructed.

With regards to the journal requirements item 1-3, we have made the related changes to comply with items 1 (style requirement) and 3 (providing proper caption for supporting information and in-text citation). With regards to the item 2, we followed the suggestion to share the de-identified data files as supporting information documents.

The authors appreciate the constructive feedback and concerns shared related to the self-reporting, group formation criteria, hidden identities, and unconscious biases. We have made related changes in different sections of the manuscript in this relation to bring more clarity and improve the interpretation. Please see our detailed responses to each of the reviewers’ comments and the changes made.

Reviewer #1:

- Clarification on survey options: In the post-course survey, students were given six choices for criteria they considered when forming their lab groups: “friends,” “students sitting next to me in the lab,” “students’ GPA and familiarity with the biological concepts,” “students’ gender identity,” “students’ race/ethnicity,” and “students’ year in college.” Could you please clarify how you determined these specific options for the survey? Were they based on previous literature, preliminary research, or another rationale?

- Potential bias in group formation criteria: Regarding the criteria provided for group formation, don't you think limiting the possible choices to a relatively small number may bias the study's results? Additionally, do you consider self-reporting problematic, given the bias that political correctness may introduce? How did you address these potential biases in your research?

Authors’ response:

In the related section of the manuscript (copied below) we used the wording “including” to indicate listing only some and not all of the response choices provided to students.

“Students could select more than one option from a list of responses including the following: “friends”; “students sitting next to me in the lab”; “students’ GPA and familiarity with the biological concepts”; “students’ gender identity”; “students’ race/ethnicity”; “students’ year in college.”

Based on the reviewer 1 related comment, to improve the clarity of this section, we have included the number of answer choices provided, and shared the original question, with all answer choices, in the supporting information document (S1_file) and did in-text citation within the manuscript as well.

In terms of the logic behind the answer choices selected, in the early stage of developing the survey the authors explored a collection of STEM education research publications and brainstormed the list of most common visible and hidden demographic and academic identities students may identify and associate with. We considered the criteria that could be visible from the first day of the class when students meet each other like “racial/ethnic identity” and the ones that may be less evident or hidden but students could possibly share with each other in the first 3 weeks of the lab such as “being an international student or not”. We admit that this list might not be exhaustive, but we have tried to include here the most common visible and hidden demographic, and academic identities and background commonly studied and discussed in STEM education research. To address the reviewer’s concern, we have added a few sentences in the related section to explain our logic behind selecting these criteria for the multiple-answer question in the manuscript.

In the post-survey before students see this multiple-answer question, they were also asked to respond to an open-ended question below:

“Which criteria did you use to form your research group?”

Our intention for including this question in the survey was to explore how students’ responses may differ when they were provided a list of criteria to choose from compared to an open-ended question. Preliminary coding of students’ responses for the open-ended question showed that the “students sitting next to me in the lab” and “friends” were also the most two common criteria students shared, while other factors comprised only a very small proportion of responses. Thus, due to similar findings in open-ended and multiple-answer questions to avoid redundancy, we focused on the multiple-choice responses.

We have added a few sentences in the manuscript to communicate this part (page 10).

We agree that self-reporting may have its own limitations and as the only source of data collection might not be adequate enough in some cases. However, to study a question like this one we think hearing from students about what criteria mattered to them when they formed or joined a group is a more suitable choice. While self-report has inherent limitations, it can still provide valuable information about students’ perspectives. The purpose of RQ 1 was to provide an opportunity for students to share about their experiences and we would not want to omit their perspectives. The purpose of RQ 2 was to compare self-selected to random groupings, an analysis that does help reveal biases in students’ group formation processes.

We acknowledge that current study cannot tease apart the conscious and unconscious biases that students’ likely hold when locating themselves in group settings, interacting with new people, and navigating student-selected group formations. We also note that students may not be entirely forthcoming about their considerations. Yet, there was a consistent trend in students’ responses that proximity and friendship were relevant for their choice of lab partners. To address the reviewer’s concern, we have added a few sentences in the manuscript sharing what this study could not address.

- Sample size clarification: When you mention, "The responses of students who selected non-binary or prefer not to say options had to be eliminated from the quantitative analysis due to their small sample size," how small was this sample size? While the answer is provided in Table 1, adding this information directly to the text would make the paragraph more straightforward. Could you include the specific numbers in the text for clarity?

Authors’ response:

We agree with this reviewer’s comment thus we have added the related numbers to the text (page 12).

- Unconscious bias in seating choices: The authors state, "In this examination of student survey data, we found that the majority of students reported casually forming groups with peers who were sitting around them and that their process yielded groups with less demographic isolation than would have resulted from randomly-formed groupings." Could it be that students choose their seats based on unconscious bias towards minorities? How did your analysis account for potential unconscious biases in seating choices?

Authors’ response:

We agree it is possible that the selection of where to sit in lab could have been impacted by unconscious bias. As noted above, teasing apart conscious and unconscious biases in student decisions is beyond the scope of this study. Very few students reported that they intentionally considered demographics in their group choice, although students may not be entirely forthcoming about their considerations. Given student responses, we propose three logical possibilities: it may be the case that students did not want to report that they did consider demographics; it may also be the case that demographics influenced group choice only subconsciously; it may be the case that demographics did not generally influence students’ group formation processes. While teasing apart the first two possibilities is outside the scope of this study, our analyses comparing actual to simulated random groupings do address the third.

To address this comment and add clarity to this discussion we added some explanations to different parts of the manuscript.

Reviewer #2:

-While question 1 was addressed by the research team, there are still unaddressed questions. The fact that students working in informal groups for 3 week prior to creating teams was not addressed as a potential impact on team selection. Figure 2 shows that most students opted to work with students near them, but the fact that those students had potentially worked together and formed trust over the course of those 3 weeks is not addressed. In a follow up study, it would be interesting to look at the group of students that did not team up with those around them, presumably those students that they’ve been working with, and collect qualitative data on their reasoning for picking a team with students they did not work with during the initial 3 weeks of the lab. This could potentially help address the claim on page 21 “students can choose groups to avoid the types of demographic isolation that they think will likely cause them harm.”. While that data does show that allowing students to choose their groups can allow them to actively avoid demographic isolation, which is interpreted as a self protective mechanism, and is important in supporting historically marginalized groups in STEM, the data does not support that there is the same impact for hidden identities (claim made on page 20).

Authors’ response:

It is true that students had three weeks to get to know each other before forming groups and that could have impacted group formation. The main point of providing three weeks of interaction before forming groups, in our context, was to help students get to know each other better and ease in to working in a longer-term group. We tried to assess this by asking students a few questions in the second semester, fall 2019, post survey about how they chose where to seat in the lab and if they changed their seat between week 1 and the week they formed groups, week 4. As it was shared in page 16, students stated that on the first day of class, they chose the first available lab seat (74%) or sat by their friends (21%) in response to the questions on how they decided where to sit in the lab. The majority of students also shared not changing their seat between the first week of class and when forming their groups in week four (87%). This could indicate that the student’s initial evaluation of their peers on the first day of class based on what matters to them, could have impacted where to sit and later form groups. However, we admit that we have not assessed here the relationship between selection of where to sit in the lab and the possible impacts of trust building within the first three weeks on group formation in this study. A future study that compares students’ choices in group formation on week one vs. for example week three, when they had time to build trust, would be valuable.

To add clarity to this discussion considering what we have actually assessed, based on this comment, we have added more explanation to different parts of the manuscript, please see page 22-23.

-The two potentially hidden identities surveyed for are first generation status and international status. These two identities may not be immediately visible from someones physical appearance and showed no statistically significant difference in demographic isolation percentage between the classroom and simulated data. More information is needed for the authors to properly make this claim.

Authors’ response:

We agree that the first-generation status and international status are not as visible and easy to detect as the two other demographics. However, we think in the first three weeks of labs when students had the chance to interact with each other, and hopefully develop trust, they might have noticed or share the identities that might be hidden in their groups and seem important to them before forming groups.

As we discussed in page 20 (now page 22-23), no statistical significance in demographic isolation percentage between the classroom and simulated data could in part be explained as “Because the proportion of students who identified as first-generation or international was low in the course, there was more likelihood for those students to be isolated in lab groups, whether they self-selected or were randomly assigned.”

To add clarity to this discussion considering what we have actually assessed, based on this comment, we have added more explanation to different parts of the manuscript, please see pages 22-23.

We hope the academic editor finds our responses and related changes with regards to the reviewers’ comments satisfying and considers our manuscript, for publication in PLOS ONE.

Thank you for your consideration and looking forward to hearing from you!

Sincerely,

Mitra Asgari, Ph.D. (corresponding author)

Assistant Teaching Professor

Division of Biological Sciences, University of Missouri-Columbia

Columbia, Missouri

Email: mitra.asgari@missouri.edu

Decision Letter 1

Gabriel Velez

9 Sep 2024

Demographic isolation and attitudes toward group work in student-selected lab groups

PONE-D-24-13332R1

Dear Dr. Asgari,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Gabriel Velez, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: (No Response)

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: (No Response)

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: (No Response)

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: (No Response)

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: (No Response)

Reviewer #2: N/A

**********

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If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

**********

Acceptance letter

Gabriel Velez

16 Sep 2024

PONE-D-24-13332R1

PLOS ONE

Dear Dr. Asgari,

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now being handed over to our production team.

At this stage, our production department will prepare your paper for publication. This includes ensuring the following:

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on behalf of

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Academic Editor

PLOS ONE

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    S1 Table. Groupwork attitude items.

    (DOCX)

    pone.0310918.s001.docx (14.7KB, docx)
    S2 Table. Multi-level linear regression output for group work EAP pretest and posttest scores.

    (DOCX)

    pone.0310918.s002.docx (17.6KB, docx)
    S3 Table. Multi-level linear regression output for group work plausible values across five trials to check for accuracy.

    (DOCX)

    pone.0310918.s003.docx (24.2KB, docx)
    S1 File

    (PDF)

    pone.0310918.s004.pdf (118.7KB, pdf)
    S2 File. Data used in group selection criteria.

    (XLSX)

    pone.0310918.s005.xlsx (28.3KB, xlsx)
    S3 File. Data used in randomized grouping analyses.

    (XLSX)

    pone.0310918.s006.xlsx (33.7KB, xlsx)
    S4 File. Data used in MLM analyses.

    (CSV)

    pone.0310918.s007.csv (49.4KB, csv)

    Data Availability Statement

    This study involves human research participant data. All relevant data that were used in analyses are first de-identified and then provided as Supporting Information files to allow to replication of the results of the study.


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