Fig. 5. Inhibitory synaptic transmission is impaired in adult mice lacking adolescent oligodendrogenesis.
a, Example images of parvalbumin (PV) and WFA immunostaining in the adult visual cortex of CreER− (CTL) and CreER+ (cKO) mice. b–d, Quantification of PV+ neuron density (b, P = 0.2538), percentage of PV+ neurons with WFA+ perineuronal nets (c, P = 0.1773) and WFA intensity in visual cortex (d, P = 0.718). e, Example voltage-clamp traces recorded at 0 mV from layer V visual cortex pyramidal neurons in the presence of tetrodotoxin, d-(-)-2-amino-5-phosphonopentanoic acid (APV) and 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX). f, Example waveforms of mIPSCs (average trace obtained from all events recorded from one cell for each genotype). g, Cumulative distribution of mIPSC interevent interval. Inset, quantification of mIPSC frequency (P = 0.0003). h, Cumulative distribution of mIPSC amplitude. Inset, quantification of mIPSC amplitude (P = 0.6413). b–d, Unpaired two-tailed t-test, n = 3 CTL mice and n = 3 cKO mice; g–h, unpaired two-tailed t-test, n = 15 cells from five CTL mice and n = 14 cells from five cKO mice. Data presented as mean ± s.e.m. ***P < 0.001. Additional statistical details are provided in Supplementary in Table 1.