Figure 3.

Phylogenetic tree based on the partial B646L gene sequence. To construct the phylogenetic tree, we aligned at least one representative sequence for each genotype (black, accession number as name) and the sequences generated in our study using the Q5 DNA polymerase (red) and the Platinum SuperFi II DNA polymerase (blue), using them as input for the Geneious Tree Builder. The samples sequenced in this study all clustered to the expected genotype (green box). All Georgia 2007 and Estonia 2014 samples cluster together with other genotype II ASFV strains (LS478113 and FR682468). The Perpignan strain clustered to KM236553, which is a genotype I ASFV strain, whereas Malawi and KWH clustered to AF270707 and AY351530, respectively, genotype VIII and X strains. Of note, the cell culture-adapted strain 1819V does not cluster with any known ASFV genotypes.