Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1993 Aug;468:557–565. doi: 10.1113/jphysiol.1993.sp019788

Splenic control of intravascular volume in the rat.

S Kaufman 1, Y Deng 1
PMCID: PMC1143843  PMID: 8254524

Abstract

1. We tested the hypothesis that hypervolaemia causes an increase in intrasplenic filtration of cell-free fluid out of the vasculature. To this end we developed a preparation in the anaesthetized rat whereby the splenic vein could be non-occlusively cannulated. 2. Haematocrit and plasma protein concentrations were measured in the splenic afferent and efferent blood supplies. 3. In response to volume loading with saline (1% body weight), there was a sustained increase in the arterial-venous differential of haematocrit, i.e. there was a relative increase in the haematocrit of the blood draining from the spleen. There was no such change in plasma protein concentration. By contrast, this degree of volume loading had no effect on the haematocrit of blood passing through the hindquarters of the animal. 4. Following volume expansion, there was no significant difference in the protein concentration of the plasma and the lymph fluid collected from the splenic lymphatic duct. 5. Distension of the superior vena caval-right atrial junction by means of a small inflatable balloon, caused a similar increase in the splenic venous haematocrit, and again, no change in plasma protein concentration. 6. We interpret these results to mean that, in response to expansion of the intravascular space, there is increased intrasplenic filtration of plasma out of the blood and into the lymphatic system.

Full text

PDF
557

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barcroft J., Florey H. W. Some factors involved in the concentration of blood by the spleen. J Physiol. 1928 Nov 9;66(3):231–234. doi: 10.1113/jphysiol.1928.sp002521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carneiro J. J., Donald D. E. Blood reservoir function of dog spleen, liver, and intestine. Am J Physiol. 1977 Jan;232(1):H67–H72. doi: 10.1152/ajpheart.1977.232.1.H67. [DOI] [PubMed] [Google Scholar]
  3. Greenway C. V. Splenic erythrocyte concentration mechanism and its inhibition by isoproterenol. Am J Physiol. 1979 Feb;236(2):H238–H243. doi: 10.1152/ajpheart.1979.236.2.H238. [DOI] [PubMed] [Google Scholar]
  4. Hartwig H., Hartwig H. G. Structural characteristics of the mammalian spleen indicating storage and release of red blood cells. Aspects of evolutionary and environmental demands. Experientia. 1985 Feb 15;41(2):159–163. doi: 10.1007/BF02002608. [DOI] [PubMed] [Google Scholar]
  5. Kaufman S. Chronic, nonocclusive, and maintenance-free central venous cannula in the rat. Am J Physiol. 1980 Jul;239(1):R123–R125. doi: 10.1152/ajpregu.1980.239.1.R123. [DOI] [PubMed] [Google Scholar]
  6. Kaufman S. Role of right atrial receptors in the control of drinking in the rat. J Physiol. 1984 Apr;349:389–396. doi: 10.1113/jphysiol.1984.sp015162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaufman S. Role of spleen in ANF-induced reduction in plasma volume. Can J Physiol Pharmacol. 1992 Aug;70(8):1104–1108. doi: 10.1139/y92-153. [DOI] [PubMed] [Google Scholar]
  8. Maass-Moreno R., Rothe C. F. Carotid baroreceptor control of liver and spleen volume in cats. Am J Physiol. 1991 Jan;260(1 Pt 2):H254–H259. doi: 10.1152/ajpheart.1991.260.1.H254. [DOI] [PubMed] [Google Scholar]
  9. Opdyke D. F. Hemodynamics of blood flow through the spleen. Am J Physiol. 1970 Jul;219(1):102–106. doi: 10.1152/ajplegacy.1970.219.1.102. [DOI] [PubMed] [Google Scholar]
  10. REINHARDT W. O. Partition between lymph and urine of intravenously administered fluids. Am J Physiol. 1952 Apr;169(1):198–202. doi: 10.1152/ajplegacy.1952.169.1.198. [DOI] [PubMed] [Google Scholar]
  11. Reilly F. D. Innervation and vascular pharmacodynamics of the mammalian spleen. Experientia. 1985 Feb 15;41(2):187–192. doi: 10.1007/BF02002612. [DOI] [PubMed] [Google Scholar]
  12. Shoukas A. A., MacAnespie C. L., Brunner M. J., Watermeier L. The importance of the spleen in blood volume shifts of the systemic vascular bed caused by the carotid sinus baroreceptor reflex in the dog. Circ Res. 1981 Sep;49(3):759–766. doi: 10.1161/01.res.49.3.759. [DOI] [PubMed] [Google Scholar]
  13. Stock R. J., Cilento E. V., Reilly F. D., McCuskey R. S. A compartmental analysis of the splenic circulation in rat. Am J Physiol. 1983 Jul;245(1):H17–H21. doi: 10.1152/ajpheart.1983.245.1.H17. [DOI] [PubMed] [Google Scholar]
  14. Toth E., Stelfox J., Kaufman S. Cardiac control of salt appetite. Am J Physiol. 1987 May;252(5 Pt 2):R925–R929. doi: 10.1152/ajpregu.1987.252.5.R925. [DOI] [PubMed] [Google Scholar]
  15. Valenzuela-Rendon J., Manning R. D., Jr Chronic transvascular fluid flux and lymph flow during volume-loading hypertension. Am J Physiol. 1990 May;258(5 Pt 2):H1524–H1533. doi: 10.1152/ajpheart.1990.258.5.H1524. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES