Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1984 Jul 15;221(2):541–543. doi: 10.1042/bj2210541

Activation of Neurospora crassa soluble adenylate cyclase by calmodulin.

J A Reig, M T Téllez-Iñón, M M Flawiá, H N Torres
PMCID: PMC1144071  PMID: 6236798

Abstract

The soluble form of adenylate cyclase was extracted and purified from wild-type Neurospora crassa mycelia. Brain or N. crassa calmodulin significantly enhanced this enzyme activity in assay mixtures containing Mg2+-ATP as substrate. EGTA reverses this calmodulin activation.

Full text

PDF
541

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cox J. A., Ferraz C., Demaille J. G., Perez R. O., van Tuinen D., Marmé D. Calmodulin from neurospora crassa. General properties and conformational changes. J Biol Chem. 1982 Sep 25;257(18):10694–10700. [PubMed] [Google Scholar]
  2. Da Silveira J. F., Zingales B., Colli W. Characterization of an adenylyl cyclase activity in particulate preparations from epimastigote forms of Trypanosoma cruzi. Biochim Biophys Acta. 1977 Apr 12;481(2):722–733. doi: 10.1016/0005-2744(77)90306-0. [DOI] [PubMed] [Google Scholar]
  3. Flawiá M. M., Kornblihtt A. R., Reig J. A., Torruella M., Torres H. N. Reconstitution of a hormone-sensitive adenylate cyclase with membrane extracts from Neurospora and avian erythrocytes. J Biol Chem. 1983 Jul 10;258(13):8255–8259. [PubMed] [Google Scholar]
  4. Flawiá M. M., Torres H. N. Adenylate cyclase activity in Neurospora crassa. I. General properties. J Biol Chem. 1972 Nov 10;247(21):6873–6879. [PubMed] [Google Scholar]
  5. Gomes S. L., Mennucci L., da Costa Maia J. C. Adenylate cyclase activity and cyclic AMP metabolism during cytodifferentiation of Blastocladiella emersonii. Biochim Biophys Acta. 1978 Jun 15;541(2):190–198. doi: 10.1016/0304-4165(78)90392-6. [DOI] [PubMed] [Google Scholar]
  6. Ortega Perez R., Van Tuinen D., Marmé D., Cox J. A., Turian G. Purification and identification of calmodulin from Neurospora crassa. FEBS Lett. 1981 Oct 26;133(2):205–208. doi: 10.1016/0014-5793(81)80506-6. [DOI] [PubMed] [Google Scholar]
  7. Paveto C., Epstein A., Passeron S. Studies on cyclic adenosine 3' ,5'-monophosphate levels, Adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii. Arch Biochem Biophys. 1975 Aug;169(2):449–457. doi: 10.1016/0003-9861(75)90187-3. [DOI] [PubMed] [Google Scholar]
  8. Reig J. A., Kornblihtt A. R., Flawiá M. M., Torres H. N. Soluble adenylate cyclase activity in Neurospora crassa. Biochem J. 1982 Oct 1;207(1):43–49. doi: 10.1042/bj2070043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosenberg G. B., Pall M. L. Characterization of an ATP-Mg2+-dependent guanine nucleotide-stimulated adenylate cyclase from Neurospora crassa. Arch Biochem Biophys. 1983 Feb 15;221(1):243–253. doi: 10.1016/0003-9861(83)90141-8. [DOI] [PubMed] [Google Scholar]
  10. Ross E. M., Howlett A. C., Ferguson K. M., Gilman A. G. Reconstitution of hormone-sensitive adenylate cyclase activity with resolved components of the enzyme. J Biol Chem. 1978 Sep 25;253(18):6401–6412. [PubMed] [Google Scholar]
  11. Salter R. S., Krinks M. H., Klee C. B., Neer E. J. Calmodulin activates the isolated catalytic unit of brain adenylate cyclase. J Biol Chem. 1981 Oct 10;256(19):9830–9833. [PubMed] [Google Scholar]
  12. Van Eldik L. J., Zendegui J. G., Marshak D. R., Watterson D. M. Calcium-binding proteins and the molecular basis of calcium action. Int Rev Cytol. 1982;77:1–61. doi: 10.1016/s0074-7696(08)62463-8. [DOI] [PubMed] [Google Scholar]
  13. Varimo K., Londesborough J. Solubilization and other studies on adenylate cyclase of baker's yeast. Biochem J. 1976 Nov;159(2):363–370. doi: 10.1042/bj1590363. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES