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. 1984 Aug 15;222(1):261–264. doi: 10.1042/bj2220261

Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase.

R Mathur, A S Balasubramanian
PMCID: PMC1144168  PMID: 6477510

Abstract

A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single step with 100% recovery.

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Selected References

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  1. Alam T., Balsubramanian A. S. The purification, properties and characterization of three forms of alpha-L-fucosidase from monkey brain. Biochim Biophys Acta. 1978 Jun 9;524(2):373–384. doi: 10.1016/0005-2744(78)90174-2. [DOI] [PubMed] [Google Scholar]
  2. Autio S., Nordén N. E., Ockerman P. A., Riekkinen P., Rapola J., Louhimo T. Mannosidosis: clinical, fine-structural and biochemical findings in three cases. Acta Paediatr Scand. 1973 Nov;62(6):555–565. doi: 10.1111/j.1651-2227.1973.tb17065.x. [DOI] [PubMed] [Google Scholar]
  3. Bischoff J., Kornfeld R. Evidence for an alpha-mannosidase in endoplasmic reticulum of rat liver. J Biol Chem. 1983 Jul 10;258(13):7907–7910. [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Dawson G. Evidence for two distinct forms of mammalian beta-mannosidase. J Biol Chem. 1982 Apr 10;257(7):3369–3371. [PubMed] [Google Scholar]
  6. Hirani S., Winchester B. The multiple forms of alpha-D-mannosidase in human plasma. Biochem J. 1979 Jun 1;179(3):583–592. doi: 10.1042/bj1790583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones M. Z., Dawson G. Caprine beta-mannosidosis. Inherited deficiency of beta-D-mannosidase. J Biol Chem. 1981 May 25;256(10):5185–5188. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. MAHLER H. R. The use of amine buffers in studies with enzymes. Ann N Y Acad Sci. 1961 Jun 17;92:426–439. doi: 10.1111/j.1749-6632.1961.tb44992.x. [DOI] [PubMed] [Google Scholar]
  10. Mathur R., Balasubramanian A. S. Separation, purification, comparative properties & subcellular localization of acid & neutral alpha-D-mannosidase of monkey brain. Indian J Biochem Biophys. 1981 Oct;18(5):334–341. [PubMed] [Google Scholar]
  11. Phillips N. C., Robinson D., Winchester B. G. Characterization of human liver alpha-D-mannosidase purified by affinity chromatography. Biochem J. 1976 Mar 1;153(3):579–587. doi: 10.1042/bj1530579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Phillips N. C., Robinson D., Winchester B. G., Jolly R. D. Mannosidosis in Angus cattle. The enzymic defect. Biochem J. 1974 Feb;137(2):363–371. doi: 10.1042/bj1370363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Porath J., Carlsson J., Olsson I., Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature. 1975 Dec 18;258(5536):598–599. doi: 10.1038/258598a0. [DOI] [PubMed] [Google Scholar]
  14. Shoup V. A., Touster O. Purification and characterization of the alpha-D-mannosidase of rat liver cytosol. J Biol Chem. 1976 Jul 10;251(13):3845–3852. [PubMed] [Google Scholar]
  15. Snaith S. M. Multiple alpha-mannosidase activities in mammalian tissues as shown by metal-ion activation. Biochem J. 1977 Jun 1;163(3):557–564. doi: 10.1042/bj1630557. [DOI] [PMC free article] [PubMed] [Google Scholar]

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