Abstract
n-Octyl beta-D-thioglucopyranoside, a new non-ionic detergent, was synthesized. Properties, and applicability to membrane proteins, of this detergent were investigated. The detergent was easily removed by dialysis. The solubilizing power of this detergent for Escherichia coli membrane proteins was similar to that of n-octyl beta-D-glucopyranoside, which has been widely used in membrane biochemistry. No inactivation of proteins was observed after the solubilization. n-Octyl beta-D-thioglucopyranoside was superior to n-octyl beta-D-glucopyranoside in that it was much more stable and could be synthesized at much lower cost.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baron C., Thompson T. E. Solubilization of bacterial membrane proteins using alkyl glucosides and dioctanoyl phosphatidylcholine. Biochim Biophys Acta. 1975 Mar 25;382(3):276–285. doi: 10.1016/0005-2736(75)90270-9. [DOI] [PubMed] [Google Scholar]
- Hanatani M., Nishifuji K., Futai M., Tsuchiya T. Solubilization and reconstitution of membrane proteins of Escherichia coli using alkanoyl-N-methylglucamides. J Biochem. 1984 May;95(5):1349–1353. doi: 10.1093/oxfordjournals.jbchem.a134742. [DOI] [PubMed] [Google Scholar]
- Hildreth J. E. N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry. Biochem J. 1982 Nov 1;207(2):363–366. doi: 10.1042/bj2070363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newman M. J., Foster D. L., Wilson T. H., Kaback H. R. Purification and reconstitution of functional lactose carrier from Escherichia coli. J Biol Chem. 1981 Nov 25;256(22):11804–11808. [PubMed] [Google Scholar]
- Newman M. J., Wilson T. H. Solubilization and reconstitution of the lactose transport system from Escherichia coli. J Biol Chem. 1980 Nov 25;255(22):10583–10586. [PubMed] [Google Scholar]
- Okada T., Ueyama K., Niiya S., Kanazawa H., Futai M., Tsuchiya T. Role of inducer exclusion in preferential utilization of glucose over melibiose in diauxic growth of Escherichia coli. J Bacteriol. 1981 Jun;146(3):1030–1037. doi: 10.1128/jb.146.3.1030-1037.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROE J. H. The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem. 1955 Jan;212(1):335–343. [PubMed] [Google Scholar]
- Racker E., Violand B., O'Neal S., Alfonzo M., Telford J. Reconstitution, a way of biochemical research; some new approaches to membrane-bound enzymes. Arch Biochem Biophys. 1979 Dec;198(2):470–477. doi: 10.1016/0003-9861(79)90521-6. [DOI] [PubMed] [Google Scholar]
- Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem. 1973 Dec;56(2):502–514. doi: 10.1016/0003-2697(73)90217-0. [DOI] [PubMed] [Google Scholar]
- Tsuchiya T., Misawa A., Miyake Y., Yamasaki K., Niiya S. Solubilization and reconstitution of membrane energy-transducing systems of Escherichia coli. FEBS Lett. 1982 Jun 7;142(2):231–234. doi: 10.1016/0014-5793(82)80141-5. [DOI] [PubMed] [Google Scholar]
- Tsuchiya T., Ottina K., Moriyama Y., Newman M. J., Wilson T. H. Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli. J Biol Chem. 1982 May 10;257(9):5125–5128. [PubMed] [Google Scholar]
- Wright J. K., Overath P. Purification of the lactose:H+ carrier of Escherichia coli and characterization of galactoside binding and transport. Eur J Biochem. 1984 Feb 1;138(3):497–508. doi: 10.1111/j.1432-1033.1984.tb07944.x. [DOI] [PubMed] [Google Scholar]