Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1984 Nov 1;223(3):627–632. doi: 10.1042/bj2230627

The discovery of a rapidly metabolized polymeric tetraphosphate derivative of adenosine in perfused rat heart.

J Mowbray, W L Hutchinson, G R Tibbs, P G Morris
PMCID: PMC1144345  PMID: 6508734

Abstract

The predicted presence in perfused rat hearts of a rapidly metabolized but hitherto unrecognized form of adenosine phosphate has been confirmed by specific radioactive labelling. The properties of the purified compound suggest that it is a heteropolymer of a small organic acid, phosphate and purine nucleoside in the proportions 1:4:1.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bates D. J., Perrett D., Mowbray J. Systematic variations in the content of the purine nucleotides in the steady-state perfused rat heart. Evidence for the existence of controlled storage and release of adenine nucleotides. Biochem J. 1978 Nov 15;176(2):485–493. doi: 10.1042/bj1760485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burt C. T., Glonek T., Bárány M. Analysis of phosphate metabolites, the intracellular pH, and the state of adenosine triphosphate in intact muscle by phosphorus nuclear magnetic resonance. J Biol Chem. 1976 May 10;251(9):2584–2591. [PubMed] [Google Scholar]
  3. CALDWELL P. C., PRANKERD T. A. Phosphate esters in myotonic human muscle. J Neurol Neurosurg Psychiatry. 1954 May;17(2):127–128. doi: 10.1136/jnnp.17.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CALDWELL P. C. The separation of the phosphate esters of muscle by paper chromatography. Biochem J. 1953 Oct;55(3):458–467. doi: 10.1042/bj0550458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clegg J. S., Warner A. H., Finamore F. J. Evidence for the function of P-1, P-4-diguanosine 5'-tetraphosphate in the development of Artemia salina. J Biol Chem. 1967 Apr 25;242(8):1938–1943. [PubMed] [Google Scholar]
  6. Dawson M. J., Gadian D. G., Wilkie D. R. Contraction and recovery of living muscles studies by 31P nuclear magnetic resonance. J Physiol. 1977 Jun;267(3):703–735. doi: 10.1113/jphysiol.1977.sp011835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goh S. H., Léjohn H. B. Genetical and biochemical evidence that a novel dinucleoside polyphosphate coordinates salvage and de novo nucleotide biosynthetic pathways in mammalian cells. Biochem Biophys Res Commun. 1977 Jan 10;74(1):256–264. doi: 10.1016/0006-291x(77)91402-4. [DOI] [PubMed] [Google Scholar]
  8. Hutchinson W. L., Bates D. J., Perrett D., Mowbray J. In perfused rat hearts ischaemia promotes the reversible conversion of appreciable quantities of soluble adenine nucleotides to a stable trichloroacetic acid-precipitable form. Biosci Rep. 1981 Nov;1(11):857–863. doi: 10.1007/BF01114819. [DOI] [PubMed] [Google Scholar]
  9. JACOB M. I., BERNE R. M. Metabolism of purine derivatives by the isolated cat heart. Am J Physiol. 1960 Feb;198:322–326. doi: 10.1152/ajplegacy.1960.198.2.322. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., PASSONNEAU J. V., HASSELBERGER F. X., SCHULZ D. W. EFFECT OF ISCHEMIA ON KNOWN SUBSTRATES AND COFACTORS OF THE GLYCOLYTIC PATHWAY IN BRAIN. J Biol Chem. 1964 Jan;239:18–30. [PubMed] [Google Scholar]
  11. Liu M. S., Feinberg H. Incorporation of adenosine-8-14/C and inosine-8-14C into rabbit heart adenine nucleotides. Am J Physiol. 1971 May;220(5):1242–1248. doi: 10.1152/ajplegacy.1971.220.5.1242. [DOI] [PubMed] [Google Scholar]
  12. Mowbray J., Bates D. J., Perrett D. Inversely related oscillations in the contents of cyclic GMP and the total adenine nucleotides in steady-state perfused rat hearts. FEBS Lett. 1981 Aug 17;131(1):55–59. doi: 10.1016/0014-5793(81)80886-1. [DOI] [PubMed] [Google Scholar]
  13. Mowbray J., Ottaway J. H. The effect of insulin and growth hormone on the flux of tracer from labelled lactate in perfused rat heart. Eur J Biochem. 1973 Jul 16;36(2):369–379. doi: 10.1111/j.1432-1033.1973.tb02921.x. [DOI] [PubMed] [Google Scholar]
  14. Mowbray J., Ottaway J. H. The flux of pyruvate in perfused rat heart. Eur J Biochem. 1973 Jul 16;36(2):362–368. doi: 10.1111/j.1432-1033.1973.tb02920.x. [DOI] [PubMed] [Google Scholar]
  15. PERLMANN G. E. The nature of phosphorus linkages in phosphoproteins. Adv Protein Chem. 1955;10:1–30. doi: 10.1016/s0065-3233(08)60103-5. [DOI] [PubMed] [Google Scholar]
  16. Perrett D. Simplified low-pressure high-resolution nucleotide analyser. J Chromatogr. 1976 Sep 15;124(2):187–196. doi: 10.1016/s0021-9673(00)89735-6. [DOI] [PubMed] [Google Scholar]
  17. SERAYDARIAN K., MOMMAERTS W. F., WALLNER A. The amount and compartmentalization of adenosine diphosphate in muscle. Biochim Biophys Acta. 1962 Dec 17;65:443–460. doi: 10.1016/0006-3002(62)90447-x. [DOI] [PubMed] [Google Scholar]
  18. Seeley P. J., Busby S. J., Gadian D. G., Radda G. K., Richards R. E. A new approach to metabolite compartmentation in muscle. Biochem Soc Trans. 1976;4(1):62–64. doi: 10.1042/bst0040062. [DOI] [PubMed] [Google Scholar]
  19. Stein G., Stein J., Shephard E., Park W., Phillips I. Evidence that the coupling of histone gene expression and DNA synthesis in HeLa S3 cells is not mediated at the transscriptional level. Biochem Biophys Res Commun. 1977 Jul 11;77(1):245–252. doi: 10.1016/s0006-291x(77)80189-7. [DOI] [PubMed] [Google Scholar]
  20. Strohman R. C., Moss P. S., Micou-Eastwood J., Spector D., Przybyla A., Paterson B. Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures. Cell. 1977 Feb;10(2):265–273. doi: 10.1016/0092-8674(77)90220-3. [DOI] [PubMed] [Google Scholar]
  21. Warner A. H., McClean D. K. Studies on the biosynthesis and role of diguanosine tetraphosphate during growth and development of Artemia salina. Dev Biol. 1968 Sep;18(3):278–293. doi: 10.1016/0012-1606(68)90036-5. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES