Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1984 Nov 1;223(3):803–807. doi: 10.1042/bj2230803

Renal transport of monocarboxylic acids. Heterogeneity of lactate-transport systems along the proximal tubule.

K E Jørgensen, M I Sheikh
PMCID: PMC1144365  PMID: 6508742

Abstract

The characteristics of D- and L-lactate transport in luminal-membrane vesicles derived from whole cortex, from the pars convoluta and from the pars recta of rabbit kidney proximal tubule were studied. It was found that uptake of both isomers in vesicles from whole cortex occurred by means of dual electrogenic transport systems, namely a low-affinity system and a high-affinity system. Uptake of both isomers in vesicles from the pars recta was strictly Na+-dependent and is mediated via a single high-affinity common transport system. Vesicles from the pars convoluta contained a cation-dependent but Na+-unspecific low-affinity common transport system for these compounds. The physiological importance of this system is briefly discussed.

Full text

PDF
803

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubert L., Motais R. Molecular features of organic anion permeablity in ox red blood cell. J Physiol. 1975 Mar;246(1):159–179. doi: 10.1113/jphysiol.1975.sp010884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barac-Nieto M., Murer H., Kinne R. Lactate-sodium cotransport in rat renal brush border membranes. Am J Physiol. 1980 Nov;239(5):F496–F506. doi: 10.1152/ajprenal.1980.239.5.F496. [DOI] [PubMed] [Google Scholar]
  3. Deuticke B. Monocarboxylate transport in erythrocytes. J Membr Biol. 1982;70(2):89–103. doi: 10.1007/BF01870219. [DOI] [PubMed] [Google Scholar]
  4. Deuticke B. Properties and structural basis of simple diffusion pathways in the erythrocyte membrane. Rev Physiol Biochem Pharmacol. 1977;78:1–97. doi: 10.1007/BFb0027721. [DOI] [PubMed] [Google Scholar]
  5. Deuticke B., Rickert I., Beyer E. Stereoselective, SH-dependent transfer of lactate in mammalian erythrocytes. Biochim Biophys Acta. 1978 Feb 2;507(1):137–155. doi: 10.1016/0005-2736(78)90381-4. [DOI] [PubMed] [Google Scholar]
  6. Díes F., Ramos G., Avelar E., Lennhoff M. Renal excretion of lactic acid in the dog. Am J Physiol. 1969 Jan;216(1):106–111. doi: 10.1152/ajplegacy.1969.216.1.106. [DOI] [PubMed] [Google Scholar]
  7. Hildmann B., Storelli C., Haase W., Barac-Nieto M., Murer H. Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles. Biochem J. 1980 Jan 15;186(1):169–176. doi: 10.1042/bj1860169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Höhmann B., Frohnert P. P., Kinne R., Baumann K. Proximal tubular lactate transport in rat kidney: a micropuncture study. Kidney Int. 1974 Apr;5(4):261–270. doi: 10.1038/ki.1974.35. [DOI] [PubMed] [Google Scholar]
  9. Jørgensen K. E., Kragh-Hansen U., Røigaard-Petersen H., Sheikh M. I. Citrate uptake by basolateral and luminal membrane vesicles from rabbit kidney cortex. Am J Physiol. 1983 Jun;244(6):F686–F695. doi: 10.1152/ajprenal.1983.244.6.F686. [DOI] [PubMed] [Google Scholar]
  10. Kragh-Hansen U., Jørgensen K. E., Sheikh M. I. The use of a potential-sensitive cyanine dye for studying ion-dependent electrogenic renal transport of organic solutes. Uptake of L-malate and D-malate by luminal-membrane vesicles. Biochem J. 1982 Nov 15;208(2):369–376. doi: 10.1042/bj2080369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kragh-Hansen U., Jørgensen K. E., Sheikh M. I. The use of potential-sensitive cyanine dye for studying ion-dependent electrogenic renal transport of organic solutes. Spectrophotometric measurements. Biochem J. 1982 Nov 15;208(2):359–368. doi: 10.1042/bj2080359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kragh-Hansen U., Røigaard-Petersen H., Jacobsen C., Sheikh M. I. Renal transport of neutral amino acids. Tubular localization of Na+-dependent phenylalanine- and glucose-transport systems. Biochem J. 1984 May 15;220(1):15–24. doi: 10.1042/bj2200015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Mengual R., Leblanc G., Sudaka P. The mechanism of Na+-L-lactate cotransport by brush-border membrane vesicles from horse kidney. Analysis by isotopic exchange kinetics of a sequential model and stoichiometry. J Biol Chem. 1983 Dec 25;258(24):15071–15078. [PubMed] [Google Scholar]
  15. Mengual R., Sudaka P. The mechanism of Na+-L-lactate cotransport by brush border membrane vesicles from horse kidney: analysis of rapid equilibrium kinetics in absence of membrane potential. J Membr Biol. 1983;71(3):163–171. doi: 10.1007/BF01875457. [DOI] [PubMed] [Google Scholar]
  16. Nord E. P., Wright S. H., Kippen I., Wright E. M. Specificity of the Na+-dependent monocarboxylic acid transport pathway in rabbit renal brush border membranes. J Membr Biol. 1983;72(3):213–221. doi: 10.1007/BF01870588. [DOI] [PubMed] [Google Scholar]
  17. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  18. Røigaard-Petersen H., Sheikh M. I. Renal transport of neutral amino acids. Demonstration of Na+-independent and Na+-dependent electrogenic uptake of L-proline, hydroxy-L-proline and 5-oxo-L-proline by luminal-membrane vesicles. Biochem J. 1984 May 15;220(1):25–33. doi: 10.1042/bj2200025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schwab A. J., Bracht A., Scholz R. Transport of D-lactate in perfused rat liver. Eur J Biochem. 1979 Dec 17;102(2):537–547. doi: 10.1111/j.1432-1033.1979.tb04270.x. [DOI] [PubMed] [Google Scholar]
  20. Sheikh M. I., Kragh-Hansen U., Jørgensen K. E., Røigaard-Petersen H. An efficient method for the isolation and separation of basolateral-membrane and luminal-membrane vesicles from rabbit kidney cortex. Biochem J. 1982 Nov 15;208(2):377–382. doi: 10.1042/bj2080377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Spencer T. L., Lehninger A. L. L-lactate transport in Ehrlich ascites-tumour cells. Biochem J. 1976 Feb 15;154(2):405–414. doi: 10.1042/bj1540405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Storelli C., Corcelli A., Cassano G., Hildmann B., Murer H., Lippe C. Polar distribution of sodium-dependent and sodium-independent transport system for L-lactate in the plasma membrane of rat enterocytes. Pflugers Arch. 1980 Oct;388(1):11–16. doi: 10.1007/BF00582622. [DOI] [PubMed] [Google Scholar]
  23. Turner R. J., Moran A. Heterogeneity of sodium-dependent D-glucose transport sites along the proximal tubule: evidence from vesicle studies. Am J Physiol. 1982 Apr;242(4):F406–F414. doi: 10.1152/ajprenal.1982.242.4.F406. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES