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. 1984 Nov 1;223(3):861–869. doi: 10.1042/bj2230861

The structure of Artemia sp. haemoglobin. Cleavage of the native molecules into functional units by limited subtilisin digestion.

L Moens, D Geelen, M L Van Hauwaert, G Wolf, R Blust, R Witters, R Lontie
PMCID: PMC1144373  PMID: 6391470

Abstract

Limited subtilisin digestion of the high-Mr haemoglobin of the crustacean Artemia sp. results in a series of fragments that are multiples of Mr 16000. Properties such as amino acid composition, iron content, absorption and c.d. spectra of the 16000-Mr functional units strongly resemble those of the intact haemoglobin molecules. The 16000-Mr functional units can bind O2 in a non-co-operative way. They thus represent the structural units from which the globin chains are built up.

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Selected References

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