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. 1984 Dec 15;224(3):955–960. doi: 10.1042/bj2240955

Functional changes in rat liver mitochondria on administration of 2-methyl-4-dimethylaminoazobenzene.

P Saikumar, C K Kurup
PMCID: PMC1144533  PMID: 6441570

Abstract

Administration of 2-methyl-4-dimethylaminobenzene in the diet (0.1%, w/w) for 85-90 days doubled the content of mitochondria in the livers of rats. The azodye was covalently bound to liver proteins, and about 15% of the amount found in liver was associated with the mitochondrial fraction. Mitochondria isolated from the livers of azodye-fed animals showed drastically lowered ability to oxidize NAD+-linked substrates. The inhibited electron-transfer step was the reduction of ubiquinone. The organelles showed a large increase in succinate oxidase activity. The activity of cytochrome oxidase and the content of cytochrome aa3 were substantially higher in these organelles. Azodye-fed animals showed depressed serum cholesterol concentrations. The content of ubiquinone in liver also registered a small increase.

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Selected References

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