Abstract
Lubricin was isolated from bovine ankle, metacarpophalangeal and knee and human knee synovial fluids. The lubricins isolated from the bovine joint fluids had the same amino acid and carbohydrate compositions, but differences were observed in the relative molecular masses. The Mr values of bovine metacarpophalangeal and ankle lubricin determined by light-scattering measurements were about 200 000, whereas values of 132 000 and 143 000 were obtained for the bovine knee lubricin. The human knee lubricin had a similar carbohydrate composition to bovine knee lubricin except for the higher glucosamine content, and the amino acid composition differed slightly. The human sample had a lower glutamic acid content and a leucine/isoleucine ratio of 2:1 compared with 1:1 in the bovine. The Mr value of the human knee lubricin (166 000) was also lower than that of the bovine metacarpophalangeal and ankle samples. The Mr value of the bovine knee lubricin determined by sedimentation-equilibrium measurements was 171 000. The length measurements determined by electron microscopy and also the sedimentation measurements showed considerable polydispersity and indicate that the degree of extension of lubricin molecules can vary. Friction measurements showed that the human knee synovial-fluid lubricin had equivalent lubricating ability in a test system in vitro to that observed for lubricin isolated from normal bovine synovial fluids. The lubricating ability of lubricin was concentration-dependent, and each lubricin sample was able to act as a lubricant in vitro in an equivalent manner to whole synovial fluid at concentrations that are thought to occur in vivo.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
- Jourdian G. W., Dean L., Roseman S. The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J Biol Chem. 1971 Jan 25;246(2):430–435. [PubMed] [Google Scholar]
- Margossian S. S., Stafford W. F., 3rd Calcium-induced dimerization of troponin-C. J Biol Chem. 1982 Feb 10;257(3):1160–1165. [PubMed] [Google Scholar]
- Roark D. E., Yphantis D. A. Studies of self-associating systems by equilibrium ultracentrifugation. Ann N Y Acad Sci. 1969 Nov 7;164(1):245–278. doi: 10.1111/j.1749-6632.1969.tb14043.x. [DOI] [PubMed] [Google Scholar]
- Silver F. H. Type I collagen fibrillogenesis in vitro. Additional evidence for the assembly mechanism. J Biol Chem. 1981 May 25;256(10):4973–4977. [PubMed] [Google Scholar]
- Slayter H. S. Electron microscopic studies of fibrinogen structure: historical perspectives and recent experiments. Ann N Y Acad Sci. 1983 Jun 27;408:131–145. doi: 10.1111/j.1749-6632.1983.tb23241.x. [DOI] [PubMed] [Google Scholar]
- Swann D. A., Bloch K. J., Swindell D., Shore E. The lubricating activity of human synovial fluids. Arthritis Rheum. 1984 May;27(5):552–556. doi: 10.1002/art.1780270511. [DOI] [PubMed] [Google Scholar]
- Swann D. A., Hendren R. B., Radin E. L., Sotman S. L., Duda E. A. The lubricating activity of synovial fluid glycoproteins. Arthritis Rheum. 1981 Jan;24(1):22–30. doi: 10.1002/art.1780240104. [DOI] [PubMed] [Google Scholar]
- Swann D. A., Slayter H. S., Silver F. H. The molecular structure of lubricating glycoprotein-I, the boundary lubricant for articular cartilage. J Biol Chem. 1981 Jun 10;256(11):5921–5925. [PubMed] [Google Scholar]
- Swann D. A., Sotman S., Dixon M., Brooks C. The isolation and partial characterization of the major glycoprotein (LGP-I) from the articular lubricating fraction from bovine synovial fluid. Biochem J. 1977 Mar 1;161(3):473–485. doi: 10.1042/bj1610473. [DOI] [PMC free article] [PubMed] [Google Scholar]