Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Jan 15;225(2):307–313. doi: 10.1042/bj2250307

Hepatic delta 6-desaturase activity in lean and genetically obese ob/ob mice.

S Hughes, D A York
PMCID: PMC1144591  PMID: 3977836

Abstract

Hepatic delta 6-desaturase activity is primarily located in the mitochondrial fraction in mice. Both delta 6- and delta 5-desaturase activities are increased in the liver of young (6-week-old) obese mice. The increase in hepatic delta 6-desaturase activity in obese mice does not occur until weaning. Neither restriction of food intake nor hyperinsulinaemia normalize hepatic delta 6-desaturase activity of obese mice. Both cold acclimation and tri-iodothyronine (30 micrograms/day per kg) decreased hepatic delta 6-desaturase activity of obese mice to levels observed in lean mice, whereas the increase in activity in obese mice was still maintained after the induction of hypothyroidism.

Full text

PDF
307

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Bray G. A., York D. A. Hypothalamic and genetic obesity in experimental animals: an autonomic and endocrine hypothesis. Physiol Rev. 1979 Jul;59(3):719–809. doi: 10.1152/physrev.1979.59.3.719. [DOI] [PubMed] [Google Scholar]
  3. Brenner R. R., Peluffo R. O., Mercuri O., Restelli M. A. Effect of arachidonic acid in the alloxan-diabetic rat. Am J Physiol. 1968 Jul;215(1):63–70. doi: 10.1152/ajplegacy.1968.215.1.63. [DOI] [PubMed] [Google Scholar]
  4. Brenner R. R. Regulatory function of delta6 desaturate -- key enzyme of polyunsaturated fatty acid synthesis. Adv Exp Med Biol. 1977;83:85–101. doi: 10.1007/978-1-4684-3276-3_8. [DOI] [PubMed] [Google Scholar]
  5. Chang K. J., Huang D., Cuatrecasas P. The defect in insulin receptors in obese-hyperglycemic mice: a probable accompaniment of more generalized alterations in membrane glycoproteins. Biochem Biophys Res Commun. 1975 May 19;64(2):566–573. doi: 10.1016/0006-291x(75)90359-9. [DOI] [PubMed] [Google Scholar]
  6. Cook H. W. In vitro formation of polyunsaturated fatty acids by desaturation in rat brain: some properties of the enzymes in developing brain and comparisons with liver. J Neurochem. 1978 Jun;30(6):1327–1334. doi: 10.1111/j.1471-4159.1978.tb10463.x. [DOI] [PubMed] [Google Scholar]
  7. Cuendet G. S., Loten E. G., Jeanrenaud B., Renold A. E. Decreased basal, noninsulin-stimulated glucose uptake and metabolism by skeletal soleus muscle isolated from obese-hyperglycemic (ob/ob) mice. J Clin Invest. 1976 Nov;58(5):1078–1088. doi: 10.1172/JCI108559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dahlen J. V., Porter J. W. Studies on the synthesis of fatty acids by a beef heart mitochondrial enzyme system. Arch Biochem Biophys. 1968 Sep 20;127(1):207–223. doi: 10.1016/0003-9861(68)90218-x. [DOI] [PubMed] [Google Scholar]
  9. Dehaye J. P., Winand J., Christophe J. Adenylate cyclase activity in the epididymal adipose tissue from obese-hyperglycaemic mice. Diabetologia. 1978 Jul;15(1):45–51. doi: 10.1007/BF01219328. [DOI] [PubMed] [Google Scholar]
  10. Enser M., Roberts J. L. The regulation of hepatic stearoyl-coenzyme A desaturase in obese-hyperglycaemic (ob/ob) mice by food intake and the fatty acid composition of the diet. Biochem J. 1982 Sep 15;206(3):561–570. doi: 10.1042/bj2060561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Enser M. The role of insulin in the regulation of stearic acid desaturase activity in liver and adipose tissue from obese--hyperglycaemic (ob/ob) and lean mice. Biochem J. 1979 Jun 15;180(3):551–558. doi: 10.1042/bj1800551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Faas F. H., Carter W. J. Fatty acid desaturation and microsomal lipid fatty acid composition in experimental hypothyroidism. Biochem J. 1982 Oct 1;207(1):29–35. doi: 10.1042/bj2070029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Faas F. H., Carter W. J. Fatty-acid desaturation and microsomal lipid fatty-acid composition in experimental hyperthyroidism. Biochem J. 1981 Mar 1;193(3):845–852. doi: 10.1042/bj1930845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. French R. R., York D. A., Portman J. M., Isaacs K. Hepatic plasma membranes from genetically obese (ob/ob) mice: studies on fluorescence polarization, phospholipid composition and 5'-nucleotidase activity. Comp Biochem Physiol B. 1983;76(2):309–319. doi: 10.1016/0305-0491(83)90075-5. [DOI] [PubMed] [Google Scholar]
  15. French R. R., York D. A. The regulation of adenylate cyclase in adipocyte plasma membrane from genetically obese (ob/ob) mice. Diabetologia. 1984 Jun;26(6):466–472. doi: 10.1007/BF00262223. [DOI] [PubMed] [Google Scholar]
  16. Holt S., York D. A. The effect of adrenalectomy on GDP binding to brown-adipose-tissue mitochondria of obese rat. Biochem J. 1982 Dec 15;208(3):819–822. doi: 10.1042/bj2080819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hughes S., York D. A. [Na+ + K+] ATP-ase in liver and brain of obese mice. Horm Metab Res. 1983 Jul;15(7):335–339. doi: 10.1055/s-2007-1018712. [DOI] [PubMed] [Google Scholar]
  18. Hyslop P. A., York D. A., Corina D. L. Changes in the composition and fluidity of membranes in obese (ob/ob) mice: a study of hepatic microsomal NADPH-cytochrome P450 oxidoreductase activity. Int J Obes. 1982;6(3):279–289. [PubMed] [Google Scholar]
  19. Hyslop P. A., York D. A. Membrane fluidity and adenylate cyclase activity in genetically obese mice. Biochem Biophys Res Commun. 1980 Feb 12;92(3):819–824. doi: 10.1016/0006-291x(80)90776-7. [DOI] [PubMed] [Google Scholar]
  20. Ida Chen Y. D., Hoch F. L. Thryoid control over biomembranes. Rat liver mitochondrial inner membranes. Arch Biochem Biophys. 1977 Jun;181(2):470–483. doi: 10.1016/0003-9861(77)90253-3. [DOI] [PubMed] [Google Scholar]
  21. Lin M. H., Vander Tuig J. G., Romsos D. R., Akera T., Leveille G. A. Na+,K+-ATPase enzyme units in lean and obese (ob/ob) thyroxine-injected mice. Am J Physiol. 1979 Sep;237(3):E265–E272. doi: 10.1152/ajpendo.1979.237.3.E265. [DOI] [PubMed] [Google Scholar]
  22. Mobley P. W., Dubuc P. U. Thyroid hormone levels in the developing obese-hyperglycemic syndrome. Horm Metab Res. 1979 Jan;11(1):37–39. doi: 10.1055/s-0028-1092679. [DOI] [PubMed] [Google Scholar]
  23. Naughton J. M. Supply of polyenoic fatty acids to the mammalian brain: the ease of conversion of the short-chain essential fatty acids to their longer chain polyunsaturated metabolites in liver, brain, placenta and blood. Int J Biochem. 1981;13(1):21–32. doi: 10.1016/0020-711x(81)90132-4. [DOI] [PubMed] [Google Scholar]
  24. Patton J. F., Platner W. S. Effects of thyroxine and cold-acclimation on hepatic fatty acid metabolism in the hamster. Proc Soc Exp Biol Med. 1971 May;137(1):196–201. doi: 10.3181/00379727-137-35543. [DOI] [PubMed] [Google Scholar]
  25. Peluffo R. O., Nervi A. M., Brenner R. R. Linoleic acid desaturation activity of liver microsomes of essential fatty acid deficient and sufficient rats. Biochim Biophys Acta. 1976 Jul 20;441(1):25–31. doi: 10.1016/0005-2760(76)90277-0. [DOI] [PubMed] [Google Scholar]
  26. Rath E. A., Thenen S. W. Use of tritiated water for measurement of 24-hour milk intake in suckling lean and genetically obese (ob/ob) mice. J Nutr. 1979 May;109(5):840–847. doi: 10.1093/jn/109.5.840. [DOI] [PubMed] [Google Scholar]
  27. Rouer E., Dansette P., Beaune P., Leroux J. P. Membrane fluidity and drug metabolism in liver microsomes of lean, ob/ob and db/db mice. Biochem Biophys Res Commun. 1980 Jul 16;95(1):41–46. doi: 10.1016/0006-291x(80)90701-9. [DOI] [PubMed] [Google Scholar]
  28. Sandermann H., Jr Regulation of membrane enzymes by lipids. Biochim Biophys Acta. 1978 Sep 29;515(3):209–237. doi: 10.1016/0304-4157(78)90015-1. [DOI] [PubMed] [Google Scholar]
  29. Sprecher H. Biosynthetic pathways of polyunsaturated fatty acids. Adv Exp Med Biol. 1977;83:35–50. doi: 10.1007/978-1-4684-3276-3_5. [DOI] [PubMed] [Google Scholar]
  30. Trayhurn P., James W. P. Thermoregulation and non-shivering thermogenesis in the genetically obese (ob/ob) mouse. Pflugers Arch. 1978 Feb 22;373(2):189–193. doi: 10.1007/BF00584859. [DOI] [PubMed] [Google Scholar]
  31. Wahle K. W., Radcliffe J. D. Effect of a diet rich in sunflower oil on aspects of lipid metabolism in the genetically-obese rat. Lipids. 1977 Feb;12(2):135–139. doi: 10.1007/BF02533282. [DOI] [PubMed] [Google Scholar]
  32. Winand J., Furnelle J., Wodon C., Hebbelinck M., Christophe J. 7-Day time study of lipid metabolism in normal and obese-hyperglycemic Bar Harbor mice. Qualitative and quantitative aspects. Biochimie. 1973;55(1):63–73. doi: 10.1016/s0300-9084(73)80238-x. [DOI] [PubMed] [Google Scholar]
  33. York D. A., Hyslop P. A., French R. Fluorescence polarisation and composition of membranes in genetic obesity. Biochem Biophys Res Commun. 1982 Jun 30;106(4):1478–1453. doi: 10.1016/0006-291x(82)91280-3. [DOI] [PubMed] [Google Scholar]
  34. York D. A., Otto W., Taylor T. G. Thyroid status of obese (ob/ob) mice and its relationship to adipose tissue metabolism. Comp Biochem Physiol B. 1978;59(1):59–65. doi: 10.1016/0305-0491(78)90271-7. [DOI] [PubMed] [Google Scholar]
  35. de Gómez Dumm I. N., de Alaniz M. J., Brenner R. R. Effect of thyroxine on delta 6 and delta 9 desaturation activity. Adv Exp Med Biol. 1977;83:609–616. [PubMed] [Google Scholar]
  36. van der Kroon P. H., Boldewijn H., Langeveld-Soeter N. Congenital hypothyroidism in latent obese (ob/ob) mice. Int J Obes. 1982;6(1):83–90. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES