Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Jan 15;225(2):529–533. doi: 10.1042/bj2250529

Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells.

A J Strain, W A Wallace, A H Wyllie
PMCID: PMC1144620  PMID: 2983677

Abstract

Synchronized CV-1 cells were transfected with SV40 (simian virus 40) DNA-calcium phosphate co-precipitates. In the presence of carrier DNA, the transfection efficiency of SV40 DNA was decreased 5-fold in S-phase cells and was increased 4-fold in preparations of mitotically enriched cells as compared with asynchronous controls. No difference was observed when carrier DNA was omitted, when cells had progressed through S-phase and into G2-phase, or when the infectivity of cells to intact SV40 virus was tested. These results highlight the importance of cell-cycle-dependent factors on DNA-mediated gene transfer.

Full text

PDF
529

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fraley R., Straubinger R. M., Rule G., Springer E. L., Papahadjopoulos D. Liposome-mediated delivery of deoxyribonucleic acid to cells: enhanced efficiency of delivery related to lipid composition and incubation conditions. Biochemistry. 1981 Nov 24;20(24):6978–6987. doi: 10.1021/bi00527a031. [DOI] [PubMed] [Google Scholar]
  2. Giulotto E., Israel N. DNA-mediated gene transfer is more efficient during S-phase of the cell cycle. Biochem Biophys Res Commun. 1984 Jan 13;118(1):310–316. doi: 10.1016/0006-291x(84)91102-1. [DOI] [PubMed] [Google Scholar]
  3. Graessmann A., Graessmann M., Mueller C. Regulation of SV40 gene expression. Adv Cancer Res. 1981;35:111–149. doi: 10.1016/s0065-230x(08)60910-0. [DOI] [PubMed] [Google Scholar]
  4. Graessmann A., Graessmann M., Mueller C. Regulatory mechanism of simian virus 40 gene expression in permissive and in nonpermissive cells. J Virol. 1976 Mar;17(3):854–858. doi: 10.1128/jvi.17.3.854-858.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graessmann A., Wolf H., Bornkamm G. W. Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA. Proc Natl Acad Sci U S A. 1980 Jan;77(1):433–436. doi: 10.1073/pnas.77.1.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henry S. M., Hodge L. D. Nuclear matrix: a cell-cycle-dependent site of increased intranuclear protein phosphorylation. Eur J Biochem. 1983 Jun 1;133(1):23–29. doi: 10.1111/j.1432-1033.1983.tb07425.x. [DOI] [PubMed] [Google Scholar]
  7. Laskey R. A. Phosphorylation of nuclear proteins. Philos Trans R Soc Lond B Biol Sci. 1983 Jul 5;302(1108):143–150. doi: 10.1098/rstb.1983.0047. [DOI] [PubMed] [Google Scholar]
  8. Luthman H., Magnusson G. High efficiency polyoma DNA transfection of chloroquine treated cells. Nucleic Acids Res. 1983 Mar 11;11(5):1295–1308. doi: 10.1093/nar/11.5.1295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Perucho M., Hanahan D., Wigler M. Genetic and physical linkage of exogenous sequences in transformed cells. Cell. 1980 Nov;22(1 Pt 1):309–317. doi: 10.1016/0092-8674(80)90178-6. [DOI] [PubMed] [Google Scholar]
  10. Pfeiffer S. E., Tolmach L. J. Inhibition of DNA synthesis in HeLa cells by hydroxyurea. Cancer Res. 1967 Jan;27(1):124–129. [PubMed] [Google Scholar]
  11. ROBBINS E., MARCUS P. I. MITOTICALLY SYNCHRONIZED MAMMALIAN CELLS: A SIMPLE METHOD FOR OBTAINING LARGE POPULATIONS. Science. 1964 May 29;144(3622):1152–1153. doi: 10.1126/science.144.3622.1152. [DOI] [PubMed] [Google Scholar]
  12. SINCLAIR W. K., MORTON R. A. VARIATIONS IN X-RAY RESPONSE DURING THE DIVISION CYCLE OF PARTIALLY SYNCHRONIZED CHINESE HAMSTER CELLS IN CULTURE. Nature. 1963 Sep 21;199:1158–1160. doi: 10.1038/1991158a0. [DOI] [PubMed] [Google Scholar]
  13. Scangos G., Ruddle F. H. Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review. Gene. 1981 Jun-Jul;14(1-2):1–10. doi: 10.1016/0378-1119(81)90143-8. [DOI] [PubMed] [Google Scholar]
  14. Strain A. J., Wyllie A. H. The uptake and stability of simian-virus-40 DNA after calcium phosphate transfection of CV-1 cells. Biochem J. 1984 Mar 1;218(2):475–482. doi: 10.1042/bj2180475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TERASIMA T., TOLMACH L. J. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res. 1963 Apr;30:344–362. doi: 10.1016/0014-4827(63)90306-9. [DOI] [PubMed] [Google Scholar]
  16. Wyllie A. H., Gurdon J. B., Price J. Nuclear localisation of an oocyte component required for the stability of injected DNA. Nature. 1977 Jul 14;268(5616):150–152. doi: 10.1038/268150a0. [DOI] [PubMed] [Google Scholar]
  17. Zucker R. M., D'Alisa R. M., Gershey E. L. Characterization of a CV-1 cell cycle. III. Biophysical parameters. Exp Cell Res. 1979 Aug;122(1):1–8. doi: 10.1016/0014-4827(79)90553-6. [DOI] [PubMed] [Google Scholar]
  18. de Duve C., de Barsy T., Poole B., Trouet A., Tulkens P., Van Hoof F. Commentary. Lysosomotropic agents. Biochem Pharmacol. 1974 Sep 15;23(18):2495–2531. doi: 10.1016/0006-2952(74)90174-9. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES