Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1985 Feb 15;226(1):29–35. doi: 10.1042/bj2260029

Integrated rate equations for irreversible enzyme-catalysed first-order and second-order reactions.

E A Boeker
PMCID: PMC1144673  PMID: 3977872

Abstract

Integrated rate equations are presented that describe irreversible enzyme-catalysed first-order and second-order reactions. The equations are independent of the detailed mechanism of the reaction, requiring only that it be hyperbolic and unbranched. The results should be directly applicable in the laboratory.

Full text

PDF
29

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boeker E. A. Integrated rate equations for enzyme-catalysed first-order and second-order reactions. Biochem J. 1984 Oct 1;223(1):15–22. doi: 10.1042/bj2230015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darvey I. G., Shrager R., Kohn L. D. Integrated steady state rate equations and the determination of individual rate constants. J Biol Chem. 1975 Jun 25;250(12):4696–4701. [PubMed] [Google Scholar]
  3. Duggleby R. G. A nonlinear regression program for small computers. Anal Biochem. 1981 Jan 1;110(1):9–18. doi: 10.1016/0003-2697(81)90104-4. [DOI] [PubMed] [Google Scholar]
  4. Duggleby R. G., Morrison J. F. Progress curve analysis in enzyme kinetics: model discrimination and parameter estimation. Biochim Biophys Acta. 1978 Oct 12;526(2):398–409. doi: 10.1016/0005-2744(78)90131-6. [DOI] [PubMed] [Google Scholar]
  5. Duggleby R. G., Morrison J. F. The analysis of progress curves for enzyme-catalysed reactions by non-linear regression. Biochim Biophys Acta. 1977 Apr 12;481(2):297–312. doi: 10.1016/0005-2744(77)90264-9. [DOI] [PubMed] [Google Scholar]
  6. Fernley H. N. Statistical estimations in enzyme kinetics. The integrated Michaelis equation. Eur J Biochem. 1974 Apr 1;43(2):377–378. doi: 10.1111/j.1432-1033.1974.tb03423.x. [DOI] [PubMed] [Google Scholar]
  7. JOHANSEN G., LUMRY R. Statistical analysis of enzymic steady-state rate data. C R Trav Lab Carlsberg. 1961;32:185–214. [PubMed] [Google Scholar]
  8. Orsi B. A., Tipton K. F. Kinetic analysis of progress curves. Methods Enzymol. 1979;63:159–183. doi: 10.1016/0076-6879(79)63010-0. [DOI] [PubMed] [Google Scholar]
  9. Selwyn M. J. A simple test for inactivation of an enzyme during assay. Biochim Biophys Acta. 1965 Jul 29;105(1):193–195. doi: 10.1016/s0926-6593(65)80190-4. [DOI] [PubMed] [Google Scholar]
  10. WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES