Abstract
[1-14C]Dolichol mixed in vitro with rat serum and injected intravenously into rats was rapidly cleared from the circulation in a manner consistent with a two-compartment model. About 80% of the radioactivity recovered from animals killed after 1 day was in the liver, with smaller amounts being found in lung, carcass (internal organs removed), gastrointestinal tract and contents, and spleen. The kidneys, testes and heart contained little radioactivity, and the brain did not appear to take up any [1-14C]dolichol. The half-life for the turnover of radioactivity from [1-14C]dolichol in tissues varied considerably, being 2 days for the lung, 17 for liver and about 50 days for the carcass. After 1 day, and also after 4 and 21 days, most of the radioactivity in all tissues was as [1-14C]dolichol and as [1-14C]dolichyl fatty acyl ester, although a small amount of incorporation of [1-14C]dolichol radioactivity into phospholipids was also observed. Faeces collected over the first 4 days after injection contained 13% of the [1-14C]dolichol dose, but urine and expired air contained only small amounts of radioactivity. Radioactivity in faeces was nearly all as unchanged [1-14C]dolichol and as [1-14C]dolichyl fatty acyl ester. The [1-14C]dolichol remaining in liver after 21 days appeared to be in a pool (possibly lysosomes) where most of it was not subject to excretion.
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- BURGOS J., HEMMING F. W., PENNOCK J. F., MORTON R. A. DOLICHOL: A NATURALLY-OCCURRING C100 ISOPRENOID ALCOHOL. Biochem J. 1963 Sep;88:470–482. [PMC free article] [PubMed] [Google Scholar]
- Hubbard S. C., Ivatt R. J. Synthesis and processing of asparagine-linked oligosaccharides. Annu Rev Biochem. 1981;50:555–583. doi: 10.1146/annurev.bi.50.070181.003011. [DOI] [PubMed] [Google Scholar]
- Keenan R. W., Fischer J. B., Kruczek M. E. The tissue and subcellular distribution of [3H]dolichol in the rat. Arch Biochem Biophys. 1977 Mar;179(2):634–642. doi: 10.1016/0003-9861(77)90152-7. [DOI] [PubMed] [Google Scholar]
- Keenan R. W., Kruczek M. E., Fischer J. B. The binding of [3H]dolichol by plasma high density lipoproteins. Biochim Biophys Acta. 1976 Jan 18;486(1):1–9. doi: 10.1016/0005-2760(77)90064-9. [DOI] [PubMed] [Google Scholar]
- Kim J. J., Hamilton R. M., Carroll K. K. Effects of diet on catabolism and excretion of (26-14C)cholesterol in rats. Can J Biochem. 1976 Mar;54(3):272–279. doi: 10.1139/o76-040. [DOI] [PubMed] [Google Scholar]
- Redgrave T. G., Roberts D. C., West C. E. Separation of plasma lipoproteins by density-gradient ultracentrifugation. Anal Biochem. 1975 May 12;65(1-2):42–49. doi: 10.1016/0003-2697(75)90488-1. [DOI] [PubMed] [Google Scholar]
- Rip J. W., Chaudhary N., Carroll K. K. Distribution and metabolism of dolichol and dolichyl phosphate in rat liver. Can J Biochem Cell Biol. 1983 Sep;61(9):1025–1031. doi: 10.1139/o83-131. [DOI] [PubMed] [Google Scholar]
- Rip J. W., Chaudhary N., Carroll K. K. The submicrosomal distribution of dolichyl phosphate and dolichyl phosphate phosphatase in rat liver. J Biol Chem. 1983 Dec 25;258(24):14926–14930. [PubMed] [Google Scholar]
- Rip J. W., Rupar C. A., Chaudhary N., Carroll K. K. Localization of a dolichyl phosphate phosphatase in plasma membranes of rat liver. J Biol Chem. 1981 Feb 25;256(4):1929–1934. [PubMed] [Google Scholar]
- Terpstra A. H., Woodward C. J., Sanchez-Muniz F. J. Improved techniques for the separation of serum lipoproteins by density gradient ultracentrifugation: visualization by prestaining and rapid separation of serum lipoproteins from small volumes of serum. Anal Biochem. 1981 Feb;111(1):149–157. doi: 10.1016/0003-2697(81)90243-8. [DOI] [PubMed] [Google Scholar]
- Wong T. K., Decker G. L., Lennarz W. J. Localization of dolichol in the lysosomal fraction of rat liver. J Biol Chem. 1982 Jun 10;257(11):6614–6618. [PubMed] [Google Scholar]