Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 May 15;228(1):27–33. doi: 10.1042/bj2280027

Re-utilization of pyrimidine nucleotides during rat liver regeneration.

E N Nikolov, M D Dabeva
PMCID: PMC1144949  PMID: 2408609

Abstract

The changes in the specific radioactivities of the pool of total acid-soluble uridine nucleotides and of uridine and cytidine components of total cellular and nuclear RNA were monitored in regenerating rat liver for 12 days after partial hepatectomy. Evidence is presented for the re-utilization of pyrimidine nucleotides derived from cytoplasmic RNA degradation for the synthesis of new RNA. The extent of recycling was assessed and the true rate of rRNA turnover determined more accurately. The reutilization of the uridine components of RNA was 7.0%/day during the proliferative and 3.2%/day during the post-proliferative phase, whereas that of the cytidine nucleotides was more pronounced (9.6%/day and 18.1%/day respectively). The results reveal the existence of partial compartmentalization of pyrimidine ribonucleoside triphosphate pools in the nucleus and cytoplasm of rat liver cells.

Full text

PDF
27

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birch B., Turnock G. Transfer RNA and ribosomal RNA are synthesized from the same pyrimidine nucleotide pool. Biochem J. 1982 Oct 15;208(1):89–92. doi: 10.1042/bj2080089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blobel G., Potter V. R. Distribution of radioactivity between the acid-soluble pool and the pools of RNA in the nuclear, nonsedimethable and ribosome fractions of rat liver after a single injection of lebaled orotic acid. Biochim Biophys Acta. 1968 Aug 23;166(1):48–57. doi: 10.1016/0005-2787(68)90489-9. [DOI] [PubMed] [Google Scholar]
  3. Bucher N. L., Swaffield M. N. Nucleotide pools and [6-14C]orotic acid incorporation in early regenerating rat liver. Biochim Biophys Acta. 1966 Dec 21;129(3):445–459. doi: 10.1016/0005-2787(66)90060-8. [DOI] [PubMed] [Google Scholar]
  4. Dabeva M. D., Dudov K. P., Hadjiolov A. A., Stoykova A. S. Quantitative analysis of rat liver nucleolar and nucleoplasmic ribosomal ribonucleic acids. Biochem J. 1978 May 1;171(2):367–374. doi: 10.1042/bj1710367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dabeva M. D., Petrov P. T., Stoykova A. S., Hadjiolov A. A. Contamination of detergent-purified rat liver nuclei by cytoplasmic ribosomes. Exp Cell Res. 1977 Sep;108(2):467–471. doi: 10.1016/s0014-4827(77)80061-x. [DOI] [PubMed] [Google Scholar]
  6. Eliceiri G. L. Turnover of ribosomal RNA in liver. Biochim Biophys Acta. 1976 Nov 1;447(4):391–394. doi: 10.1016/0005-2787(76)90076-9. [DOI] [PubMed] [Google Scholar]
  7. Falkenthal S., Lengyel J. A. Measurement of ribonucleotide pool specific activities by an in vivo method: comparison with an in vitro method. Biochemistry. 1981 Jan 20;20(2):312–319. doi: 10.1021/bi00505a013. [DOI] [PubMed] [Google Scholar]
  8. Genchev D. D. Free pyrimidine nucleotide pool of Ehrlich ascites-tumour cells. Characteristics related to quantitative studies of RNA metabolism. Biochem J. 1980 Apr 15;188(1):75–83. doi: 10.1042/bj1880075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Genchev D. D., Kermekchiev M. B., Hadjiolov A. A. Free pyrimidine nucleotide pool of Ehrlich ascites-tumour cells. Compartmentation with respect to the synthesis of heterogeneous nuclear RNA and precursors to ribosomal RNA. Biochem J. 1980 Apr 15;188(1):85–90. doi: 10.1042/bj1880085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goodlad G. A., Ma G. Y. Ribosomal RNA turnover and the level of ribonuclease activity in the liver of the pregnant rat. Biochim Biophys Acta. 1975 Jan 20;378(2):221–229. doi: 10.1016/0005-2787(75)90110-0. [DOI] [PubMed] [Google Scholar]
  11. Hauschka P. V. Analysis of nucleotide pools in animal cells. Methods Cell Biol. 1973;7:361–462. doi: 10.1016/s0091-679x(08)61787-2. [DOI] [PubMed] [Google Scholar]
  12. Hirsch C. A., Hiatt H. H. Turnover of liver ribosomes in fed and in fasted rats. J Biol Chem. 1966 Dec 25;241(24):5936–5940. [PubMed] [Google Scholar]
  13. KATZ S., COMB D. G. A NEW METHOD FOR THE DETERMINATION OF THE BASE COMPOSITION OF RIBONUCLEIC ACID. J Biol Chem. 1963 Sep;238:3065–3067. [PubMed] [Google Scholar]
  14. Khym J. X., Jones M. H., Lee W. H., Regan J. D., Volkin E. On the question of compartmentalization of the nucleotide pool. J Biol Chem. 1978 Dec 25;253(24):8741–8746. [PubMed] [Google Scholar]
  15. Kumagai H., Igarashi K., Tanaka K., Nakao H., Hirose S. A microsomal exoribonuclease from rat liver. Biochim Biophys Acta. 1979 Jan 12;566(1):192–199. doi: 10.1016/0005-2744(79)90261-4. [DOI] [PubMed] [Google Scholar]
  16. Loeb J. N., Howell R. R., Tomkins G. M. Turnover of ribosomal RNA in rat liver. Science. 1965 Sep 3;149(3688):1093–1095. doi: 10.1126/science.149.3688.1093. [DOI] [PubMed] [Google Scholar]
  17. Loeb J. N., Yeung L. L. Synthesis and degradation of ribosomal RNA in regenerating liver. J Exp Med. 1975 Sep 1;142(3):575–587. doi: 10.1084/jem.142.3.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Melvin W. T., Kumar A., Malt R. A. Conservation of ribosomal RNA during compensatory renal hypertrophy. A major mechanism in RNA accretion. J Cell Biol. 1976 Jun;69(3):548–556. doi: 10.1083/jcb.69.3.548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nikolov E. N., Dabeva M. D., Nikolov T. K. Turnover of ribosomes in regenerating rat liver. Int J Biochem. 1983;15(10):1255–1260. doi: 10.1016/0020-711x(83)90215-x. [DOI] [PubMed] [Google Scholar]
  20. Nikolov E. N., Dabeva M. D. Turnover of ribosomal 28S and 18S rRNA during rat liver regeneration. Biosci Rep. 1983 Aug;3(8):781–788. doi: 10.1007/BF01120990. [DOI] [PubMed] [Google Scholar]
  21. Nordgren H., Stenram U. Decreased half-life of the RNA of free and membrane-bound ribosomes in the liver of protein-deprived rats. Hoppe Seylers Z Physiol Chem. 1972 Dec;353(12):1832–1836. doi: 10.1515/bchm2.1972.353.2.1832. [DOI] [PubMed] [Google Scholar]
  22. Plagemann P. G. Nucleotide pools in Novikoff rat hepatoma cells growing in suspension culture. 3. Effects of nucleosides in medium on levels of nucleotides in separate nucleotide pools for nuclear and cytoplasmic RNA synthesis. J Cell Biol. 1972 Jan;52(1):131–146. doi: 10.1083/jcb.52.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Plagemann P. G. Nucleotide pools of Novikoff rat hepatoma cells growing in suspension culture. II. Independent nucleotide pools for nucleic acid synthesis. J Cell Physiol. 1971 Apr;77(2):241–248. doi: 10.1002/jcp.1040770213. [DOI] [PubMed] [Google Scholar]
  24. Puckett L., Darnell J. E. Essential factors in the kinetic analysis of RNA synthesis in HeLa cells. J Cell Physiol. 1977 Mar;90(3):521–534. doi: 10.1002/jcp.1040900315. [DOI] [PubMed] [Google Scholar]
  25. Retz K. C., Steele W. J. Ribosome turnover in rat brain and liver. Life Sci. 1980 Dec 22;27(25-26):2601–2604. doi: 10.1016/0024-3205(80)90546-9. [DOI] [PubMed] [Google Scholar]
  26. Seifert J. Reutilization of preformed pyrimidines for the synthesis of DNA in rat liver and kidney. Eur J Biochem. 1982 Nov;128(1):277–280. doi: 10.1111/j.1432-1033.1982.tb06962.x. [DOI] [PubMed] [Google Scholar]
  27. Seifert J., Vácha J. Differences in the turnover of uridylic and cytidylic acids of rat liver cytoplasmic ribosomes. Arch Biochem Biophys. 1974 Jan;160(1):285–288. doi: 10.1016/s0003-9861(74)80036-6. [DOI] [PubMed] [Google Scholar]
  28. Soeiro R., Ehrenfeld E. Cytoplasmic and nuclear pyrimidine ribonucleotide pools in HeLa cells. J Mol Biol. 1973 Jun 15;77(1):177–187. doi: 10.1016/0022-2836(73)90371-9. [DOI] [PubMed] [Google Scholar]
  29. Sporn M. B., Lazarus H. M., Smith J. M., Henderson W. R. Studies on nuclear exoribonucleases. 3. Isolation and properties of the enzyme from normal and malignant tissues of the mouse. Biochemistry. 1969 Apr;8(4):1698–1706. doi: 10.1021/bi00832a053. [DOI] [PubMed] [Google Scholar]
  30. Tsurugi K., Morita T., Ogata K. Mode of degradation of ribosomes in regenerating rat liver in vivo. Eur J Biochem. 1974 Jun 1;45(1):119–126. doi: 10.1111/j.1432-1033.1974.tb03536.x. [DOI] [PubMed] [Google Scholar]
  31. Vácha J., Seifert J. Turnover of cytidine and uridine components of acid-soluble pool and RNA of cytoplasmic ribosomes after repeated phenobarbital administration. Biochem Pharmacol. 1975 Feb 1;24(3):401–405. doi: 10.1016/0006-2952(75)90225-7. [DOI] [PubMed] [Google Scholar]
  32. Wiegers U., Kramer G., Klapproth K., Hilz H. Separate pyrimidine-nucleotide pools for messenger-RNA and ribosomal-RNA synthesis in HeLa S3 cells. Eur J Biochem. 1976 May 1;64(2):535–540. doi: 10.1111/j.1432-1033.1976.tb10333.x. [DOI] [PubMed] [Google Scholar]
  33. Wilson S. H., Hoagland M. B. Physiology of rat-liver polysomes. The stability of messenger ribonucleic acid and ribosomes. Biochem J. 1967 May;103(2):556–566. doi: 10.1042/bj1030556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wu R. S., Soeiro R. Turnover of nuclear RNA in HeLa cells: evidence for a single ribonucleotide pool. J Mol Biol. 1971 Jun 14;58(2):481–487. doi: 10.1016/0022-2836(71)90365-2. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES