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. 1985 May 15;228(1):147–153. doi: 10.1042/bj2280147

Multiple proteins related to the soluble galactose-binding animal lectin revealed by a monoclonal anti-lectin antibody.

S R Carding, R A Childs, R Thorpe, M Spitz, T Feizi
PMCID: PMC1144963  PMID: 4004810

Abstract

A monoclonal antibody (NIBy 142-36/8) raised against the soluble galactose-binding lectin of bovine heart muscle has been tested by solid-phase vinyl-plate radiobinding and nitrocellulose immunoblotting with homogenates of various bovine tissues, and the muscle tissues of pig, rabbit, chicken and rat. Muscle lectins of chicken, rabbit and rat differed from those of man and pig in their lack of reactivity with the 36/8 antibody. There was a good correlation of haemagglutinating activities and immunoreactivities of the bovine tissue homogenates, suggesting that the soluble galactose-binding protein is a major haemagglutinin in various tissues. Immunoblotting experiments revealed an array of antigenically active components in the homogenates in addition to the 13 and 26kDa proteins that were previously detected in preparations of purified lectin. These were in the range 36kDa to more than 200kDa, and a different spectrum of immunoreactive components was found in various cell types. Galactose-binding activity was demonstrable in 13, 26 and 36kDa components in certain bovine tissues, suggesting that the immunoreactive components of higher Mr may be inactive precursor forms of the lectin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barondes S. H. Soluble lectins: a new class of extracellular proteins. Science. 1984 Mar 23;223(4642):1259–1264. doi: 10.1126/science.6367039. [DOI] [PubMed] [Google Scholar]
  2. Beyer E. C., Zweig S. E., Barondes S. H. Two lactose binding lectins from chicken tissues. Purified lectin from intestine is different from those in liver and muscle. J Biol Chem. 1980 May 10;255(9):4236–4239. [PubMed] [Google Scholar]
  3. Carding S. R., Thorpe R., Childs R. A., Spitz M., Feizi T. Production and characterization of monoclonal antibodies to beta-galactoside-binding lectin of bovine heart muscle. Direct evidence that haemagglutinating activity is associated with a 13kDa protein. Biochem J. 1984 May 15;220(1):253–260. doi: 10.1042/bj2200253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cerra R. F., Haywood-Reid P. L., Barondes S. H. Endogenous mammalian lectin localized extracellularly in lung elastic fibers. J Cell Biol. 1984 Apr;98(4):1580–1589. doi: 10.1083/jcb.98.4.1580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Childs R. A., Feizi T. beta-Galactoside-binding muscle lectins of man and monkey show antigenic cross-reactions with those of bovine origin. Biochem J. 1979 Dec 1;183(3):755–758. doi: 10.1042/bj1830755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Feizi T., Kabat E. A. Immunochemical studies on blood groups. LVI. Purification of glycoproteins with different I determinants from hydatid cyst fluid and from human milk on insoluble anti-I immunoadsorbents. J Immunol. 1974 Jan;112(1):145–150. [PubMed] [Google Scholar]
  7. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Oda Y., Kasai K. Purification and characterization of beta-galactoside-binding lectin from chick embryonic skin. Biochim Biophys Acta. 1983 Dec 27;761(3):237–245. doi: 10.1016/0304-4165(83)90071-5. [DOI] [PubMed] [Google Scholar]
  11. Wood E., Lecomte J., Childs R. A., Feizi T. A radioimmunoassay for the measurement of blood group Ii activities: its application to glycoconjugates, oligosaccharides and intact cells. Mol Immunol. 1979 Oct;16(10):813–819. doi: 10.1016/0161-5890(79)90160-3. [DOI] [PubMed] [Google Scholar]
  12. de Waard A., Hickman S., Kornfeld S. Isolation and properties of beta-galactoside binding lectins of calf heart and lung. J Biol Chem. 1976 Dec 10;251(23):7581–7587. [PubMed] [Google Scholar]

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