Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 May 15;228(1):277–280. doi: 10.1042/bj2280277

Effects of phorbol esters on alpha 1-adrenergic-mediated and glucagon-mediated actions in isolated rat hepatocytes.

J A García-Sáinz, F Mendlovic, M A Martínez-Olmedo
PMCID: PMC1144981  PMID: 4004815

Abstract

Phorbol 12-myristate 13-acetate (PMA) inhibited the stimulation of ureogenesis produced by adrenaline, but produced a minimal displacement to the right of the dose-response curve for glucagon. However, PMA diminished the accumulation of cyclic AMP induced by glucagon. Dissociation between the cyclic AMP concentrations and the metabolic effects induced by glucagon is evidenced in the presence of phorbol esters.

Full text

PDF
277

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berry M. N., Friend D. S. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969 Dec;43(3):506–520. doi: 10.1083/jcb.43.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnbaum M. J., Fain J. N. Activation of protein kinase and glycogen phosphorylase in isolated rat liver cells by glucagon and catecholamines. J Biol Chem. 1977 Jan 25;252(2):528–535. [PubMed] [Google Scholar]
  3. Brown B. L., Albano J. D., Ekins R. P., Sgherzi A. M. A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate. Biochem J. 1971 Feb;121(3):561–562. doi: 10.1042/bj1210561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Castagna M., Takai Y., Kaibuchi K., Sano K., Kikkawa U., Nishizuka Y. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982 Jul 10;257(13):7847–7851. [PubMed] [Google Scholar]
  5. Cochet C., Gill G. N., Meisenhelder J., Cooper J. A., Hunter T. C-kinase phosphorylates the epidermal growth factor receptor and reduces its epidermal growth factor-stimulated tyrosine protein kinase activity. J Biol Chem. 1984 Feb 25;259(4):2553–2558. [PubMed] [Google Scholar]
  6. Corvera S., García-Sáinz J. A. Alpha 1-adrenoceptor activation stimulates ureogenesis in rat hepatocytes. Eur J Pharmacol. 1981 Jul 10;72(4):387–390. doi: 10.1016/0014-2999(81)90582-3. [DOI] [PubMed] [Google Scholar]
  7. Corvera S., García-Sáinz J. A. Phorbol esters inhibit alpha 1 adrenergic stimulation of glycogenolysis in isolated rat hepatocytes. Biochem Biophys Res Commun. 1984 Mar 30;119(3):1128–1133. doi: 10.1016/0006-291x(84)90892-1. [DOI] [PubMed] [Google Scholar]
  8. Corvera S., Huerta-Bahena J., Pelton J. T., Hruby V. J., Trivedi D., García-Sáinz J. A. Metabolic effects and cyclic AMP levels produced by glucagon, (1-N alpha-Trinitrophenylhistidine,12-homoarginine)glucagon and forskolin in isolated rat hepatocytes. Biochim Biophys Acta. 1984 Aug 17;804(4):434–441. doi: 10.1016/0167-4889(84)90071-5. [DOI] [PubMed] [Google Scholar]
  9. Cárdenas-Tanús R. J., Huerta-Bahena J., García-Sáinz J. A. Angiotensin II inhibits the accumulation of cyclic AMP produced by glucagon but not its metabolic effects. FEBS Lett. 1982 Jun 21;143(1):1–4. doi: 10.1016/0014-5793(82)80259-7. [DOI] [PubMed] [Google Scholar]
  10. Davis R. J., Czech M. P. Tumor-promoting phorbol diesters mediate phosphorylation of the epidermal growth factor receptor. J Biol Chem. 1984 Jul 10;259(13):8545–8549. [PubMed] [Google Scholar]
  11. Fain J. N., Shepherd R. E. Adenosine, cyclic AMP metabolism, and glycogenolysis in rat liver cells. J Biol Chem. 1977 Nov 25;252(22):8066–8070. [PubMed] [Google Scholar]
  12. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grunberger G., Gorden P. Affinity alteration of insulin receptor induced by a phorbol ester. Am J Physiol. 1982 Oct;243(4):E319–E324. doi: 10.1152/ajpendo.1982.243.4.E319. [DOI] [PubMed] [Google Scholar]
  14. Heyworth C. M., Whetton A. D., Kinsella A. R., Houslay M. D. The phorbol ester, TPA inhibits glucagon-stimulated adenylate cyclase activity. FEBS Lett. 1984 May 7;170(1):38–42. doi: 10.1016/0014-5793(84)81364-2. [DOI] [PubMed] [Google Scholar]
  15. Iwashita S., Fox C. F. Epidermal growth factor and potent phorbol tumor promoters induce epidermal growth factor receptor phosphorylation in a similar but distinctively different manner in human epidermoid carcinoma A431 cells. J Biol Chem. 1984 Feb 25;259(4):2559–2567. [PubMed] [Google Scholar]
  16. Khan B. A., Bregman M. D., Nugent C. A., Hruby V. J., Brendel K. (Des-histidine 1) (N epsilon-phenylthiocarbamoyllysine 12)-glucagon: effects on glycogenolysis in perfused rat liver. Biochem Biophys Res Commun. 1980 Apr 14;93(3):729–736. doi: 10.1016/0006-291x(80)91138-9. [DOI] [PubMed] [Google Scholar]
  17. Kikkawa U., Takai Y., Tanaka Y., Miyake R., Nishizuka Y. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters. J Biol Chem. 1983 Oct 10;258(19):11442–11445. [PubMed] [Google Scholar]
  18. May W. S., Jacobs S., Cuatrecasas P. Association of phorbol ester-induced hyperphosphorylation and reversible regulation of transferrin membrane receptors in HL60 cells. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2016–2020. doi: 10.1073/pnas.81.7.2016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moon S. O., Palfrey H. C., King A. C. Phorbol esters potentiate tyrosine phosphorylation of epidermal growth factor receptors in A431 membranes by a calcium-independent mechanism. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2298–2302. doi: 10.1073/pnas.81.8.2298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Okajima F., Ui M. Lack of correlation between hormonal effects on cyclic AMP and glycogenolysis in rat liver. Arch Biochem Biophys. 1976 Aug;175(2):549–557. doi: 10.1016/0003-9861(76)90544-0. [DOI] [PubMed] [Google Scholar]
  21. Shoyab M., De Larco J. E., Todaro G. J. Biologically active phorbol esters specifically alter affinity of epidermal growth factor membrane receptors. Nature. 1979 May 31;279(5712):387–391. doi: 10.1038/279387a0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES