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. 1985 Jun 15;228(3):757–760. doi: 10.1042/bj2280757

Digitonin-collagenase perfusion for efficient separation of periportal or perivenous hepatocytes.

K O Lindros, K E Penttilä
PMCID: PMC1145048  PMID: 2992454

Abstract

Intact rat liver cells from the perivenous region were isolated by collagenase perfusion after first destroying the periportal region by a brief portal infusion of digitonin. Periportal cells were isolated after retrograde digitonin infusion. Significantly higher alanine aminotransferase, gamma-glutamyltransferase and lactate dehydrogenase activities and lower glutamate dehydrogenase and pyruvate kinase activities in periportal than in perivenous cells demonstrate marked separation. The high yield allows further characterization in vitro of the cell populations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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