Abstract
High concentrations of aldehydes slow the inactivation of cytoplasmic aldehyde dehydrogenase by disulfiram and also slow the reaction of the enzyme with 2,2'-dithiodipyridine. It is concluded that a low-affinity aldehyde-binding site is probably the site at which thiol-group modifiers react with aldehyde dehydrogenase, as well as being the active site for hydrolysis of 4-nitrophenyl acetate.
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Selected References
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- Blackwell L. F., Bennett A. F., Buckley P. D. Relationship between the mechanisms of the esterase and dehydrogenase activities of the cytoplasmic aldehyde dehydrogenase from sheep liver. An alternative view. Biochemistry. 1983 Aug 2;22(16):3784–3791. doi: 10.1021/bi00285a011. [DOI] [PubMed] [Google Scholar]
- Dickinson F. M., Hart G. J., Kitson T. M. The use of pH-gradient ion-exchange chromatography to separate sheep liver cytoplasmic aldehyde dehydrogenase from mitochondrial enzyme contamination, and observations on the interaction between the pure cytoplasmic enzyme and disulfiram. Biochem J. 1981 Dec 1;199(3):573–579. doi: 10.1042/bj1990573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kitson T. M. Further studies of the action of disulfiram and 2,2'-dithiodipyridine on the dehydrogenase and esterase activities of sheep liver cytoplasmic aldehyde dehydrogenase. Biochem J. 1982 Jun 1;203(3):743–754. doi: 10.1042/bj2030743. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kitson T. M. Methyltetrazolethiol and the Antabuse reaction. Lancet. 1984 Dec 8;2(8415):1338–1338. doi: 10.1016/s0140-6736(84)90844-4. [DOI] [PubMed] [Google Scholar]
- Kitson T. M. The activation of aldehyde dehydrogenase by diethylstilboestrol and 2,2'-dithiodipyridine. Biochem J. 1982 Oct 1;207(1):81–89. doi: 10.1042/bj2070081. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kitson T. M. The time course of the interaction of sheep liver cytoplasmic aldehyde dehydrogenase with 2,2'- and 4,4'-dithiodipyridine: a comparison with the action of disulfiram. Arch Biochem Biophys. 1984 Nov 1;234(2):487–496. doi: 10.1016/0003-9861(84)90296-0. [DOI] [PubMed] [Google Scholar]
- MacGibbon A. K., Haylock S. J., Buckley P. D., Blackwell L. F. Kinetic studies on the esterase activity of cytoplasmic sheep liver aldehyde dehydrogenase. Biochem J. 1978 Jun 1;171(3):533–538. doi: 10.1042/bj1710533. [DOI] [PMC free article] [PubMed] [Google Scholar]