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. 1985 Jul 1;229(1):87–90. doi: 10.1042/bj2290087

Preparation of reduced bovine Cu,Zn superoxide dismutase.

P Viglino, M Scarpa, D Cocco, A Rigo
PMCID: PMC1145153  PMID: 2994632

Abstract

N.m.r. and e.p.r. were used to measure the oxidation state of copper in Cu,Zn superoxide dismutase treated with reducing agents such as NaBH4, K4Fe(CN)6, Na2S2O4 and H2O2. The activity and the electrophoretic pattern of the treated enzyme were also studied. On the basis of the reducing ability and of the absence of inactivating effects, NaBH4 was the most suitable reducer of those tested. Some characteristics of the reduction of superoxide dismutase by NaBH4 were further investigated. The results obtained indicate that NaBH4 can be used to prepare, in a few minutes, solutions of completely reduced enzyme without any apparent change of the activity and of the structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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